sk br 3 cancer cell line (Genecopoeia)

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Genecopoeia sk br 3 cancer cell line

Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or <t>SK-BR-3</t> cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.

Sk Br 3 Cancer Cell Line, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sl032/SK-BR-3+human+breast+adenocarcinoma+cancer+cell+line+dual-labeled+with+luciferase+and+GFP/pmc09097604-43-2-9
Average 94 stars, based on 2 article reviews

sk br 3 cancer cell line - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy"

Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy

Journal: Frontiers in Oncology

doi: 10.3389/fonc.2022.871390

Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or SK-BR-3 cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.

Figure Legend Snippet: Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or SK-BR-3 cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.

Techniques Used: Expressing, Incubation, Derivative Assay

Mast cells bind to, penetrate, degranulate, and induce FcεRI-dependent apoptosis of BC cells. CellTracker™-Deep Red labelled ADMC (A, B) or BDMC (C) (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (A) or anti-HER2/ neu IgE (B, C) , washed, and incubated with MitoTracker™-Red HER2/ neu + BT474-1261 in culture medium containing a fluorescent apoptosis detecting dye (CellEvent Caspase 3/7; 1:100). Cells were monitored in a live cell chamber attached to a confocal microscope for up to 4 days. (D) . The amount of fluorescent intensity of the apoptosis dye was quantified using Image J of the average of ten fields of view at 63x magnification (±SD, *P < 0.05) (E) . Higher magnifications of several degranulating MCs is shown to illustrate the release and spreading of MC granules upon cancer cell HER2/ neu -induced activation of FcεRI. The red arrows indicate spreading MC granules within the BT474-1261 cancer cell mass. (F–H) . MitoTracker™-Red labelled MCs (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (F) or anti-HER2/ neu IgE (G, H) , washed, and incubated with HER2/ neu + BT474-1261 (F, G) or SK-BR-3 (H) cells and monitored in a live cell chamber for the indicated times. The red arrows indicate the dye-labelled ADMC, black arrows indicate cancer cell apoptotic bodies, and black circle indicates the outline of the ADMC within the tumor cell mass. Experiments are representative of 3 different donors.

Figure Legend Snippet: Mast cells bind to, penetrate, degranulate, and induce FcεRI-dependent apoptosis of BC cells. CellTracker™-Deep Red labelled ADMC (A, B) or BDMC (C) (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (A) or anti-HER2/ neu IgE (B, C) , washed, and incubated with MitoTracker™-Red HER2/ neu + BT474-1261 in culture medium containing a fluorescent apoptosis detecting dye (CellEvent Caspase 3/7; 1:100). Cells were monitored in a live cell chamber attached to a confocal microscope for up to 4 days. (D) . The amount of fluorescent intensity of the apoptosis dye was quantified using Image J of the average of ten fields of view at 63x magnification (±SD, *P < 0.05) (E) . Higher magnifications of several degranulating MCs is shown to illustrate the release and spreading of MC granules upon cancer cell HER2/ neu -induced activation of FcεRI. The red arrows indicate spreading MC granules within the BT474-1261 cancer cell mass. (F–H) . MitoTracker™-Red labelled MCs (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (F) or anti-HER2/ neu IgE (G, H) , washed, and incubated with HER2/ neu + BT474-1261 (F, G) or SK-BR-3 (H) cells and monitored in a live cell chamber for the indicated times. The red arrows indicate the dye-labelled ADMC, black arrows indicate cancer cell apoptotic bodies, and black circle indicates the outline of the ADMC within the tumor cell mass. Experiments are representative of 3 different donors.

Techniques Used: Incubation, Microscopy, Activation Assay

Labeling:

Article Title: A New Class of Anionic Near-Infrared Fluorescent Dyes with 1,6-Diheterophenalene-2,5-dione End Groups
Article Snippet: .. SKBR3-GFP-Luc cells (Dual labeled stable SK-BR-3 expressing green fluorescent protein (GFP) and luciferase, SL032, GeneCopoeia) were cultured in Dulbecco’s Modified Eagle Medium (DMEM high glucose, Gibco) supplemented with 10% Fetal Bovine Serum (FBS), 1% penicillin/streptomycin and 5 μg/mL puromycin, and maintained in a humidified incubator at 37 °C with 5% CO2 atmosphere. .. For cell imaging experiments, GFP labeled HER2+ human breast cancer cells were plated in Fluorobrite DMEM media (Gibco) supplemented with 10% FBS and 1% pen/strep in an 8-well chamber slide (6.0 × 104 cells in 200 μL media per well).

Expressing:

Luciferase:

Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy.
Article Snippet: .. Luciferase/GFP dual-labeled SKBR-3 cancer cell line was purchased from GeneCopoeia, Inc. Rockville, MD. .. To assess the ability of anti-HER2/neu IgE sensitized MCs to induce cell death of HER2/neu expressing tumor cells, MCs (1.5 x 105) were sensitized with 0.1 mg/ml of anti-HER2/neu IgE or psIgE for 2 h. Filtered (40 mm) BT474-1261 or SK-BR-3 breast cancer cells (5 x 104) on coverslips were labeled with MitoTrackerTM Red (1 mM, ThermoFisher Scientific) for one hour.

Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy
Article Snippet: .. Luciferase/GFP dual-labeled SK-BR-3 cancer cell line was purchased from GeneCopoeia, Inc. Rockville, MD. .. To assess the ability of anti-HER2/ neu IgE sensitized MCs to induce cell death of HER2/ neu expressing tumor cells, MCs (1.5 x 10 5 ) were sensitized with 0.1 μg/ml of anti-HER2/ neu IgE or psIgE for 2 h. Filtered (40 μm) BT474-1261 or SK-BR-3 breast cancer cells (5 x 10 4 ) on coverslips were labeled with MitoTrackerTM Red (1 μM, ThermoFisher Scientific) for one hour.

Cell Culture:

Modification:

other:

Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy.
Article Snippet: After 1 h, cells were washed and filtered (40 mm) HER2/neu-positive BT474 (ATCC, Manassas, VA) or SK-BR-3 (SL032; Genecopoeia, Inc, Rockville, MD) cells were added at the indicated number and mediator release and apoptosis measured as described (21).

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