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luciferase gfp dual labeled skbr 3 cancer cell line  (Genecopoeia)


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    Genecopoeia luciferase gfp dual labeled skbr 3 cancer cell line
    Luciferase Gfp Dual Labeled Skbr 3 Cancer Cell Line, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sl032/SK-BR-3+human+breast+adenocarcinoma+cancer+cell+line+dual-labeled+with+luciferase+and+GFP/pm35574362-50-0-9
    Average 94 stars, based on 2 article reviews
    luciferase gfp dual labeled skbr 3 cancer cell line - by Bioz Stars, 2026-11
    94/100 stars

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    other:

    Article Title: A New Class of Anionic Near-Infrared Fluorescent Dyes with 1,6-Diheterophenalene-2,5-dione End Groups
    Article Snippet: The cells were then washed briefly with DPBS (Gibco, 300 μL × 2), and fixed with 4% paraformaldehyde (pH 7.1, 400 μL in PBS) for 15 min at room temp.

    Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy.
    Article Snippet: After 1 h, cells were washed and filtered (40 mm) HER2/neu-positive BT474 (ATCC, Manassas, VA) or SK-BR-3 (SL032; Genecopoeia, Inc, Rockville, MD) cells were added at the indicated number and mediator release and apoptosis measured as described (21).

    Luciferase:

    Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy.
    Article Snippet: .. Luciferase/GFP dual-labeled SKBR-3 cancer cell line was purchased from GeneCopoeia, Inc. Rockville, MD. .. To assess the ability of anti-HER2/neu IgE sensitized MCs to induce cell death of HER2/neu expressing tumor cells, MCs (1.5 x 105) were sensitized with 0.1 mg/ml of anti-HER2/neu IgE or psIgE for 2 h. Filtered (40 mm) BT474-1261 or SK-BR-3 breast cancer cells (5 x 104) on coverslips were labeled with MitoTrackerTM Red (1 mM, ThermoFisher Scientific) for one hour.

    Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy
    Article Snippet: .. Luciferase/GFP dual-labeled SK-BR-3 cancer cell line was purchased from GeneCopoeia, Inc. Rockville, MD. .. To assess the ability of anti-HER2/ neu IgE sensitized MCs to induce cell death of HER2/ neu expressing tumor cells, MCs (1.5 x 10 5 ) were sensitized with 0.1 μg/ml of anti-HER2/ neu IgE or psIgE for 2 h. Filtered (40 μm) BT474-1261 or SK-BR-3 breast cancer cells (5 x 10 4 ) on coverslips were labeled with MitoTrackerTM Red (1 μM, ThermoFisher Scientific) for one hour.



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    Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or <t>SK-BR-3</t> cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.
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    Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or <t>SK-BR-3</t> cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.
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    luciferase gfp dual labeled skbr 3 cancer cell line  (Genecopoeia)
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    Genecopoeia luciferase gfp dual labeled skbr 3 cancer cell line
    Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or <t>SK-BR-3</t> cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.
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    https://www.bioz.com/product/sl032/SK-BR-3+human+breast+adenocarcinoma+cancer+cell+line+dual-labeled+with+luciferase+and+GFP/pm35574362-50-0-9
    Average 94 stars, based on 1 article reviews
    luciferase gfp dual labeled skbr 3 cancer cell line - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

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    Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or SK-BR-3 cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.

    Journal: Frontiers in Oncology

    Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy

    doi: 10.3389/fonc.2022.871390

    Figure Lengend Snippet: Breast cancer cell-induced BDMC mediator release. (A) Expression of HER2/ neu on BT474-1261 or SK-BR-3 cells using FACS. BDMC were sensitized with 1 μg/ml anti-HER/ neu IgE washed, and incubated with filtered BT474-1261 or SK-BR-3 cells and degranulation ( B ; left) and cytokine release ( B ; right) assessed. Data are from a single experiment representative of experiments performed on cells derived from three separate donors. Error bars represent ±SD. * p <0.05 compared with non-IgE (spontaneous) release.

    Article Snippet: Luciferase/GFP dual-labeled SK-BR-3 cancer cell line was purchased from GeneCopoeia, Inc. Rockville, MD.

    Techniques: Expressing, Incubation, Derivative Assay

    Mast cells bind to, penetrate, degranulate, and induce FcεRI-dependent apoptosis of BC cells. CellTracker™-Deep Red labelled ADMC (A, B) or BDMC (C) (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (A) or anti-HER2/ neu IgE (B, C) , washed, and incubated with MitoTracker™-Red HER2/ neu + BT474-1261 in culture medium containing a fluorescent apoptosis detecting dye (CellEvent Caspase 3/7; 1:100). Cells were monitored in a live cell chamber attached to a confocal microscope for up to 4 days. (D) . The amount of fluorescent intensity of the apoptosis dye was quantified using Image J of the average of ten fields of view at 63x magnification (±SD, *P < 0.05) (E) . Higher magnifications of several degranulating MCs is shown to illustrate the release and spreading of MC granules upon cancer cell HER2/ neu -induced activation of FcεRI. The red arrows indicate spreading MC granules within the BT474-1261 cancer cell mass. (F–H) . MitoTracker™-Red labelled MCs (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (F) or anti-HER2/ neu IgE (G, H) , washed, and incubated with HER2/ neu + BT474-1261 (F, G) or SK-BR-3 (H) cells and monitored in a live cell chamber for the indicated times. The red arrows indicate the dye-labelled ADMC, black arrows indicate cancer cell apoptotic bodies, and black circle indicates the outline of the ADMC within the tumor cell mass. Experiments are representative of 3 different donors.

    Journal: Frontiers in Oncology

    Article Title: Human Tumor Targeted Cytotoxic Mast Cells for Cancer Immunotherapy

    doi: 10.3389/fonc.2022.871390

    Figure Lengend Snippet: Mast cells bind to, penetrate, degranulate, and induce FcεRI-dependent apoptosis of BC cells. CellTracker™-Deep Red labelled ADMC (A, B) or BDMC (C) (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (A) or anti-HER2/ neu IgE (B, C) , washed, and incubated with MitoTracker™-Red HER2/ neu + BT474-1261 in culture medium containing a fluorescent apoptosis detecting dye (CellEvent Caspase 3/7; 1:100). Cells were monitored in a live cell chamber attached to a confocal microscope for up to 4 days. (D) . The amount of fluorescent intensity of the apoptosis dye was quantified using Image J of the average of ten fields of view at 63x magnification (±SD, *P < 0.05) (E) . Higher magnifications of several degranulating MCs is shown to illustrate the release and spreading of MC granules upon cancer cell HER2/ neu -induced activation of FcεRI. The red arrows indicate spreading MC granules within the BT474-1261 cancer cell mass. (F–H) . MitoTracker™-Red labelled MCs (7.5x10 4 ) were sensitized with 1 μg/ml non-specific IgE (F) or anti-HER2/ neu IgE (G, H) , washed, and incubated with HER2/ neu + BT474-1261 (F, G) or SK-BR-3 (H) cells and monitored in a live cell chamber for the indicated times. The red arrows indicate the dye-labelled ADMC, black arrows indicate cancer cell apoptotic bodies, and black circle indicates the outline of the ADMC within the tumor cell mass. Experiments are representative of 3 different donors.

    Article Snippet: Luciferase/GFP dual-labeled SK-BR-3 cancer cell line was purchased from GeneCopoeia, Inc. Rockville, MD.

    Techniques: Incubation, Microscopy, Activation Assay