tmb single-component substrate solution (Beijing Solarbio Science)
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Beijing Solarbio Science
tmb single-component substrate solution
Tmb Single Component Substrate Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single-component+tmb+substrate+solution/tmb+single+component+substrate+solution/pmc11758404-91-9-13
Average 90 stars, based on 1 article reviews
Tmb Single Component Substrate Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single-component+tmb+substrate+solution/tmb+single+component+substrate+solution/pmc11758404-91-9-13
Average 90 stars, based on 1 article reviews
tmb single-component substrate solution - by Bioz Stars,
2026-10
90/100 stars
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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Anti-CLL1 antibody and use thereof Article Snippet: The ELISA screening steps were as follows: (1) Antigen coating: after the pure antigen human CLL1 ECD-His (human CLL1 extracellular domain with His tag) at a concentration of 50 ng/mL was diluted with the coating solution, 100 μL was added to each well of the polystyrene enzyme-linked detection plate followed by incubating at 4° C. overnight; (2) Blocking: the plate was equilibrated to room temperature the next day, washed with PBS three times, and 100 μL blocking solution was added to each well, incubated at room temperature for one hour, washed with PBS three times, and patted dry; (3) Addition sample to be tested: the fusion cell culture supernatant was taken under aseptic conditions, and added 35 ̃50 μL/well to the sealed ELISA plate, at the same time, negative control wells (no cell growth) and positive control wells (adding positive serum) were set up, incubated at room temperature for 1 hour, washed with PBST (0.5% Tween) three times, and washed with PBS twice; (4) Addition secondary antibody: the diluted enzyme-labeled secondary antibody was added in an amount of 50 μL/well, incubated at 37° C. for 30 min, washed 3 times with PBS, and patted dry; (5) Color development: two-component TMB color-developing Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. 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Immunosorbent plates (96-well format, Jet Bio-Fil, Guangzhou, China) were functionalized through overnight incubation (4 °C) with 100 ng/well of either: (a) recombinant pre-fusion stabilized F protein trimer (DS-Cav1), or (b) post-fusion conformation F protein (generously provided by Prof. Sandra Qiu, USTC), diluted in |