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Medicago single nucleus transcriptomes
Single Nucleus Transcriptomes, supplied by Medicago, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single+nucleus+transcriptomes/nucleus+single+transcriptomes/pm42269592-756-0-10
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Article Title: Research progress of omics technologies in forage breeding
Article Snippet: Long, et al., Single-nucleus transcriptomes reveal spatiotem- poral symbiotic perception and early response in Medicago, Nat.

Article Title: An NSP2-MYB module orchestrates flavonoid biosynthesis and nodule symbiosis.
Article Snippet: Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago.

Article Title: Toward telomere-to-telomere genomics in Fabaceae: Unlocking comparative and functional insights into symbiotic nitrogen fixation.
Article Snippet: Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago.

RNA Sequencing:

Article Title: Decoding plant complexity through spatially resolved multi-omics approaches
Article Snippet: .. Single-nucleus RNA-seq at early infection stages in Medicago truncatula allowed for thorough root cell-type annotation and revealed cell-type-specific transcriptional responses to Ensifer meliloti, confirming known nodulation pathways and identifying unknown genes related to cell division, signalling, and nutrient transport [166]. ..

Article Title: Decoding plant complexity through spatially resolved multi-omics approaches
Article Snippet: .. Following Nod factor treatment, time-course single-nucleus RNA-seq of Medicago truncatula roots showed quick and fleeting transcriptional reprogramming in epidermal and cortical cells. ..

Infection:

Article Title: Decoding plant complexity through spatially resolved multi-omics approaches
Article Snippet: .. Single-nucleus RNA-seq at early infection stages in Medicago truncatula allowed for thorough root cell-type annotation and revealed cell-type-specific transcriptional responses to Ensifer meliloti, confirming known nodulation pathways and identifying unknown genes related to cell division, signalling, and nutrient transport [166]. ..



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Figure 2. Transcriptomic and chromatin-accessible features of bXENs (A) Principal-component analysis (PCA) of transcriptomes of bXEN, bEPSCemb, bEPSCiPS, and bTSC. (B) Heatmap showing the marker gene expression for PrE, VE, PE, Epi, and TE from each bovine stem cell type. (C) Heatmap (left panel) showing lineage-specific expressed genes for bXENs, bEPSCs, and bTSCs, as well as the enriched signaling pathways (right panel). (D) PCA of transcriptomes of bXENs and three major lineages from days 12 to 18 in vivo bovine embryos. (E) PCA of transcriptomes of XEN and ESC from bovine, mouse, and human. Datasets of bEPSCemb and bEPSCiPS are from Zhao et al., PNAS 202123; datasets of hNESCH1, hNESCH9, hNESCiPS, hPESC, hXEN7F, and hXENGATA6OE are from Okubo et al., Nature 202420; datasets of mESC, mpXEN and mXEN are from Zhong et al., Stem Cell Research 2018.26

Journal: Cell reports

Article Title: Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation.

doi: 10.1016/j.celrep.2025.115707

Figure Lengend Snippet: Figure 2. Transcriptomic and chromatin-accessible features of bXENs (A) Principal-component analysis (PCA) of transcriptomes of bXEN, bEPSCemb, bEPSCiPS, and bTSC. (B) Heatmap showing the marker gene expression for PrE, VE, PE, Epi, and TE from each bovine stem cell type. (C) Heatmap (left panel) showing lineage-specific expressed genes for bXENs, bEPSCs, and bTSCs, as well as the enriched signaling pathways (right panel). (D) PCA of transcriptomes of bXENs and three major lineages from days 12 to 18 in vivo bovine embryos. (E) PCA of transcriptomes of XEN and ESC from bovine, mouse, and human. Datasets of bEPSCemb and bEPSCiPS are from Zhao et al., PNAS 202123; datasets of hNESCH1, hNESCH9, hNESCiPS, hPESC, hXEN7F, and hXENGATA6OE are from Okubo et al., Nature 202420; datasets of mESC, mpXEN and mXEN are from Zhong et al., Stem Cell Research 2018.26

Article Snippet: Green, SOX17; blue, SOX2; red, CDX2. (E) Blastoid formation rate from distinct protocols. (F) Blastocele diameter measurement of blastoids from distinct protocols and blastocysts. (G) Inner cell mass (ICM)/embryo ratio measurement of blastoids from distinct protocols and blastocysts. (H) Joint uniform manifold approximation and projection embedding of 10X Genomics single-nucleus transcriptomes of bovine EPTX-blastoids (green) and bovine day 8 blastocysts (blue). (I) Major cluster classification based on marker expression. (J) Dot plot indicating the expression of markers of epiblast, trophectoderm, and hypoblast. (K) Percentage of three cell types in blastoid and blastocyst.

Techniques: Marker, Gene Expression, Protein-Protein interactions, In Vivo