jurkat cells (Genecopoeia)

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Genecopoeia jurkat cells
Jurkat Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+Jurkat+stably+expressing+CRISPR+Cas9%2C+randomly+inserted%2C+single+clone/us10017758-306-6-12
Average 94 stars, based on 15 article reviews

jurkat cells - by Bioz Stars, 2026-10

94/100 stars

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CRISPR:

Article Title: Single-cell multi-gene identification of somatic mutations and gene rearrangements in cancer.
Article Snippet: .. Cell lines and induction of CRISPR mutations The Jurkat cell line (ATCC TIB-152) and a Cas9-stable version of Jurkat (SL555, GeneCopoeia, Inc., Rockville, MD) were maintained in RPMI medium supplemented with 10% FBS at 37 ◦C under standard CO 2 conditions. .. We produced an oligonucleotide pool for the guide RN A (gRN A) library (IDT, Coralville, IA).

Luciferase:

Article Title: Bach2 repression of CD36 regulates lipid-metabolism-linked effector functions in follicular B cells.
Article Snippet: .. Jurkat cells were co-transfected with the CD36 Gaussia luciferase reporter construct (GeneCopoeia) and MigR1–Bach263 or empty vector MigR1 by electroporation. .. Forty-eight hours later, luciferase activities were quantified using a Secrete Pair Gaussia Luciferase Assay kit (GeneCopoeia) according to the manufacturer’s protocols.

Construct:

Plasmid Preparation:

Electroporation:

Stable Transfection:

Article Title: A genome-wide CRISPR/Cas9 screen reveals novel positive regulators of FTY720 sensitivity in acute lymphoblastic leukemia cells
Article Snippet: .. Jurkat cells stably expressing Cas9 (Genecopoeia, Rockville, MD, catalog no. SL555) were grown following the manufacturer’s protocol. ..

Article Title: A genome-wide CRISPR/Cas9 screen reveals novel positive regulators of FTY720 sensitivity in acute lymphoblastic leukemia cells.
Article Snippet: .. Jurkat cells stably expressing Cas9 (Genecopoeia, Rockville, MD, catalog no. SL555) were grown following the manufacturer’s protocol. ..

Expressing:

Transduction:

Article Title: Engineering of CD8 + T cells with an HIV-specific synthetic notch receptor to secrete broadly therapeutic antibodies for combining antiviral humoral and cellular immune responses
Article Snippet: For transduction of 293T cells, the cells were co-infected with the lentiviruses generated from the CD4-17b SNR vector and the response element vector in six-well plates with 8 µg/mL of polybrene (Yeason, Shanghai, China) for 24 h. The transduced cells were subsequently passaged by ethylenediaminetetraacetic acid-free trypsin digestion to avoid ligand-independent Notch/synNotch activation ( ). .. For the transduction of Jurkat cells and primary CD8 + T cells, the viral supernatants from transfected 293T cells were concentrated with a lentivirus concentration solution (GeneCopoeia, Rockville, MD) according to the manufacturer’s instructions, and the cells were then transduced as described previously ( ). .. Polyclonal-engineered cell populations were stained for surface expression and then purified for both high receptor expression and high DsRed expression by FACS using the BD INFLUX cell sorter.

Article Title: Engineering of CD8 + T cells with an HIV-specific synthetic notch receptor to secrete broadly therapeutic antibodies for combining antiviral humoral and cellular immune responses.
Article Snippet: For transduction of 293T cells, the cells were co-infec ted with the lentiviruses generated from the CD4-17b SNR vector and the response element vector in six-well plates with 8 µg/mL of polybrene (Yeason, Shanghai, China) for 24 h. The transduced cells were subsequently passaged by ethylenediaminetetraacetic acid-free trypsin digestion to avoid ligand-independent Notch/synNotch activation (87). .. For the transduction of Jurkat cells and primary CD8+ T cells, the viral supernatants from transfected 293T cells were concentrated with a lentivirus concentration solution (GeneCopoeia, Rockville, MD) according to the manufacturer’s instructions, and the cells were then transduced as described previously (48). .. Polyclonal-engineered cell popula tions were stained for surface expression and then purified for both high receptor expression and high DsRed expression by FACS using the BD INFLUX cell sorter.

Transfection:

Article Title: Engineering of CD8 + T cells with an HIV-specific synthetic notch receptor to secrete broadly therapeutic antibodies for combining antiviral humoral and cellular immune responses.
Article Snippet: For transduction of 293T cells, the cells were co-infec ted with the lentiviruses generated from the CD4-17b SNR vector and the response element vector in six-well plates with 8 µg/mL of polybrene (Yeason, Shanghai, China) for 24 h. The transduced cells were subsequently passaged by ethylenediaminetetraacetic acid-free trypsin digestion to avoid ligand-independent Notch/synNotch activation (87). .. For the transduction of Jurkat cells and primary CD8+ T cells, the viral supernatants from transfected 293T cells were concentrated with a lentivirus concentration solution (GeneCopoeia, Rockville, MD) according to the manufacturer’s instructions, and the cells were then transduced as described previously (48). .. Polyclonal-engineered cell popula tions were stained for surface expression and then purified for both high receptor expression and high DsRed expression by FACS using the BD INFLUX cell sorter.

Concentration Assay:

Article Title: Engineering of CD8 + T cells with an HIV-specific synthetic notch receptor to secrete broadly therapeutic antibodies for combining antiviral humoral and cellular immune responses.
Article Snippet: For transduction of 293T cells, the cells were co-infec ted with the lentiviruses generated from the CD4-17b SNR vector and the response element vector in six-well plates with 8 µg/mL of polybrene (Yeason, Shanghai, China) for 24 h. The transduced cells were subsequently passaged by ethylenediaminetetraacetic acid-free trypsin digestion to avoid ligand-independent Notch/synNotch activation (87). .. For the transduction of Jurkat cells and primary CD8+ T cells, the viral supernatants from transfected 293T cells were concentrated with a lentivirus concentration solution (GeneCopoeia, Rockville, MD) according to the manufacturer’s instructions, and the cells were then transduced as described previously (48). .. Polyclonal-engineered cell popula tions were stained for surface expression and then purified for both high receptor expression and high DsRed expression by FACS using the BD INFLUX cell sorter.

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