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786 o cas9 cells  (Genecopoeia)


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    Genecopoeia 786 o cas9 cells
    ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O <t>Cas9</t> sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.
    786 O Cas9 Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+786-O+stably+expressing+Cas9%2C+randomly+inserted%2C+single+clone/pmc11178540-202-0-6
    Average 94 stars, based on 7 article reviews
    786 o cas9 cells - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma"

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI164249

    ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O Cas9 sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.
    Figure Legend Snippet: ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O Cas9 sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.

    Techniques Used: Staining, Transfection, Plasmid Preparation, Control, Mutagenesis, Comparison, Western Blot, Expressing, Stable Transfection, Tube Formation Assay

    Related Articles

    Plasmid Preparation:

    Article Title: circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
    Article Snippet: .. The reporter plasmid and miR-122-5p mimic ormiR-NC were co-transfected into 786-O and 769-P cells, and the luciferase activity was detected using Dual-Luciferase Reporter Assay Kit (GeneCopoeia, Rockville, MD, USA). ..

    Luciferase:

    Article Title: circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
    Article Snippet: .. The reporter plasmid and miR-122-5p mimic ormiR-NC were co-transfected into 786-O and 769-P cells, and the luciferase activity was detected using Dual-Luciferase Reporter Assay Kit (GeneCopoeia, Rockville, MD, USA). ..

    Activity Assay:

    Article Title: circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
    Article Snippet: .. The reporter plasmid and miR-122-5p mimic ormiR-NC were co-transfected into 786-O and 769-P cells, and the luciferase activity was detected using Dual-Luciferase Reporter Assay Kit (GeneCopoeia, Rockville, MD, USA). ..

    Reporter Assay:

    Article Title: circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
    Article Snippet: .. The reporter plasmid and miR-122-5p mimic ormiR-NC were co-transfected into 786-O and 769-P cells, and the luciferase activity was detected using Dual-Luciferase Reporter Assay Kit (GeneCopoeia, Rockville, MD, USA). ..

    Transfection:

    Article Title: miR-502-mediated histone methyltransferase SET8 expression is associated with clear cell renal cell carcinoma risk
    Article Snippet: The renal carcinoma 786-O cell line was purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 50 IU/ml penicillin and 50 mg/ml streptomycin (all Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C in a standard humidified incubator containing 5% CO 2 . .. The renal carcinoma 786-O cells were transfected with psi-H1- SET8 small interfering (si)RNA or psi-H1 plasmids (GeneCopoeia, Inc., Rockville, MD, USA) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. ..

    Article Title: Enhancement of Proliferation and Invasion by MicroRNA-590-5p via Targeting PBRM1 in Clear Cell Renal Carcinoma Cells
    Article Snippet: Address correspondence to Pingping Yu, Xiangya Hospital, Central-South University, Changsha 410008, China.. Tel/Fax: +86-731-13574164457; E-mail: pingpingyu2004@163.com Oncology Research, Vol.. 20, pp.

    Cell Counting:

    Article Title: Upregulation of microRNA-492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro.
    Article Snippet: .. The human ccRCC cell lines, 786-O and ACHN were treated with Aza (15.55 nM), or PBA (1.5 nM) or both in combination, with or without anti-miR-429 (20 μM) (HmiR-AN0497; Genecopoeia, Guangzhou, China), for 72 h. Cell counting kit (CCK)‐8 cell proliferation assay. ..

    Proliferation Assay:

    Article Title: Upregulation of microRNA-492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro.
    Article Snippet: .. The human ccRCC cell lines, 786-O and ACHN were treated with Aza (15.55 nM), or PBA (1.5 nM) or both in combination, with or without anti-miR-429 (20 μM) (HmiR-AN0497; Genecopoeia, Guangzhou, China), for 72 h. Cell counting kit (CCK)‐8 cell proliferation assay. ..

    Transduction:

    Article Title: Nanotechnology combined therapy: tyrosine kinase-bound gold nanorod and laser thermal ablation produce a synergistic higher treatment response of renal cell carcinoma in a murine model.
    Article Snippet: Objective: To investigate tyrosine kinase inhibitors (TKI) and gold nanorod (AuNR) paired with photothermal ablation in a human metastatic clear cell renal cell carcinoma mouse model. Nanoparticles have been successful as platform for targeted drug delivery in the treatment of urologic cancers.. Likewise, the use of nanoparticles in photothermal tumor ablation, though early in its development, has provided promising results.. Our previous in vitro studies of nanoparticles loaded with both TKI and gold nanorods and activated with photothermal ablation have demonstrated significant synergistic cell kill greater than each individual arm alone.

    Construct:

    Article Title: Nanotechnology combined therapy: tyrosine kinase-bound gold nanorod and laser thermal ablation produce a synergistic higher treatment response of renal cell carcinoma in a murine model.
    Article Snippet: Objective: To investigate tyrosine kinase inhibitors (TKI) and gold nanorod (AuNR) paired with photothermal ablation in a human metastatic clear cell renal cell carcinoma mouse model. Nanoparticles have been successful as platform for targeted drug delivery in the treatment of urologic cancers.. Likewise, the use of nanoparticles in photothermal tumor ablation, though early in its development, has provided promising results.. Our previous in vitro studies of nanoparticles loaded with both TKI and gold nanorods and activated with photothermal ablation have demonstrated significant synergistic cell kill greater than each individual arm alone.



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    Image Search Results


    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: GWAS, Marker, Single Cell, RNA Sequencing

    Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Journal: Communications Medicine

    Article Title: Autoantibody repertoire analysis in paraneoplastic pemphigus reveals novel targets linked to mucocutaneous blistering and bronchiolitis obliterans

    doi: 10.1038/s43856-025-01335-2

    Figure Lengend Snippet: a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Article Snippet: Single-cell expression data were obtained from the Human Protein Atlas.

    Techniques: Gene Expression, Expressing