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stable alpha catulin promoter gfp reporter cell lines mda mb 231  (Genecopoeia)


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    Genecopoeia stable alpha catulin promoter gfp reporter cell lines mda mb 231
    Stable Alpha Catulin Promoter Gfp Reporter Cell Lines Mda Mb 231, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+MDA-MB-231+stably+expressing+CRISPR+Cas9%2C+randomly+inserted%2C+single+clone/pmc09314412-285-2-20
    Average 94 stars, based on 66 article reviews
    stable alpha catulin promoter gfp reporter cell lines mda mb 231 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: A B7-H3–Targeted CD28 Bispecific Antibody Enhances the Activity of Anti–PD-1 and CD3 T-cell Engager Immunotherapies
    Article Snippet: .. To generate MDA-MB-231-pp65 cell lines, we transfected MDA-MB-231 cells with a lentivirus delivering CMV structural protein pp65 (GeneCopoeia; LPP-CS-GS195T-Lv183-01-400). .. Stable clones were selected with neomycin, and pp65 expression was confirmed by intracellular staining with an anti-pp65 antibody (Thermo Fisher Scientific; MA1-7597; RRID: AB_1073982).

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B.
    Article Snippet: .. MDA-MB-231 cells (7.0 × 103 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Article Title: Bio-responsive adhesive antibody delivery platform for immunotherapy and preparation method thereof
    Article Snippet: .. MDA-MB-231 human breast cancer cells (ATCC No. HTB-26), luciferase/GFP double-labeled MDA-MB-231 cells (GeneCopoeia, Rockville, MD, USA) and B16-F10 mouse melanoma cells (ATCC No. CRL-6475) were incubated in Dulbecco's modified Eagle medium (DMEM; HyClone, UT, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; HyClone) and 1% (v/v) penicillin/streptomycin. ..

    Article Title: Miro1 knockout inhibits mouse breast cancer tumorigenesis
    Article Snippet: .. MDA-MB-231 human breast cancer cells were transfected with shCtrl (OmicsLinkTM shRNA Expression Clone CSHCTR001-1-mU6; GeneCopoeia, Inc., Rockville, MD) or shMiro1 (OmicsLinkTM shRNA Expression Clone, HSH106063-mU6-a; GeneCopoeia, Inc., Rockville, MD) using the GeneJet plasmid miniprep kit (Thermo Scientific #K0502 #K0503) and the X-tremeGENE HP transfection reagent. ..

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B
    Article Snippet: .. MDA-MB-231 cells (7.0 × 10 3 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Article Title: Redirecting cytotoxic lymphocytes to breast cancer tumors via metabolite-sensing receptors
    Article Snippet: .. MDA-MB-231 cells stably expressing Cas9 were purchased from GeneCopoeia (SL515). .. Protospacer sequences of three NTC and three hEpCAM targeting sgRNAs were obtained from the Human CRISPR Knockout Pooled Library (GeCKO v2) and synthesized from IDT DNA.

    Transfection:

    Article Title: A B7-H3–Targeted CD28 Bispecific Antibody Enhances the Activity of Anti–PD-1 and CD3 T-cell Engager Immunotherapies
    Article Snippet: .. To generate MDA-MB-231-pp65 cell lines, we transfected MDA-MB-231 cells with a lentivirus delivering CMV structural protein pp65 (GeneCopoeia; LPP-CS-GS195T-Lv183-01-400). .. Stable clones were selected with neomycin, and pp65 expression was confirmed by intracellular staining with an anti-pp65 antibody (Thermo Fisher Scientific; MA1-7597; RRID: AB_1073982).

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B.
    Article Snippet: .. MDA-MB-231 cells (7.0 × 103 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Article Title: Miro1 knockout inhibits mouse breast cancer tumorigenesis
    Article Snippet: .. MDA-MB-231 human breast cancer cells were transfected with shCtrl (OmicsLinkTM shRNA Expression Clone CSHCTR001-1-mU6; GeneCopoeia, Inc., Rockville, MD) or shMiro1 (OmicsLinkTM shRNA Expression Clone, HSH106063-mU6-a; GeneCopoeia, Inc., Rockville, MD) using the GeneJet plasmid miniprep kit (Thermo Scientific #K0502 #K0503) and the X-tremeGENE HP transfection reagent. ..

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B
    Article Snippet: .. MDA-MB-231 cells (7.0 × 10 3 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Expressing:

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B.
    Article Snippet: .. MDA-MB-231 cells (7.0 × 103 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Article Title: Miro1 knockout inhibits mouse breast cancer tumorigenesis
    Article Snippet: .. MDA-MB-231 human breast cancer cells were transfected with shCtrl (OmicsLinkTM shRNA Expression Clone CSHCTR001-1-mU6; GeneCopoeia, Inc., Rockville, MD) or shMiro1 (OmicsLinkTM shRNA Expression Clone, HSH106063-mU6-a; GeneCopoeia, Inc., Rockville, MD) using the GeneJet plasmid miniprep kit (Thermo Scientific #K0502 #K0503) and the X-tremeGENE HP transfection reagent. ..

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B
    Article Snippet: .. MDA-MB-231 cells (7.0 × 10 3 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Article Title: Redirecting cytotoxic lymphocytes to breast cancer tumors via metabolite-sensing receptors
    Article Snippet: .. MDA-MB-231 cells stably expressing Cas9 were purchased from GeneCopoeia (SL515). .. Protospacer sequences of three NTC and three hEpCAM targeting sgRNAs were obtained from the Human CRISPR Knockout Pooled Library (GeCKO v2) and synthesized from IDT DNA.

    Control:

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B.
    Article Snippet: .. MDA-MB-231 cells (7.0 × 103 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Article Title: Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B
    Article Snippet: .. MDA-MB-231 cells (7.0 × 10 3 cells) were seeded into 96-well plates, and 24 h later, 1.5 μg of the miRNA 3’UTR target expression clone for human TMEM41B (NM_015012.3) (HmiT102502-MT06) or the miRNA target control vector for pEZX-MT06 (CmiT000001-MT06) (GeneCopoeia, USA) was transfected using HiPerFect at a 1:3 (v/v) ratio (DNA: HiPerFect) in Opti-MEM media. ..

    Luciferase:

    Article Title: Bio-responsive adhesive antibody delivery platform for immunotherapy and preparation method thereof
    Article Snippet: .. MDA-MB-231 human breast cancer cells (ATCC No. HTB-26), luciferase/GFP double-labeled MDA-MB-231 cells (GeneCopoeia, Rockville, MD, USA) and B16-F10 mouse melanoma cells (ATCC No. CRL-6475) were incubated in Dulbecco's modified Eagle medium (DMEM; HyClone, UT, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; HyClone) and 1% (v/v) penicillin/streptomycin. ..

    Incubation:

    Article Title: Bio-responsive adhesive antibody delivery platform for immunotherapy and preparation method thereof
    Article Snippet: .. MDA-MB-231 human breast cancer cells (ATCC No. HTB-26), luciferase/GFP double-labeled MDA-MB-231 cells (GeneCopoeia, Rockville, MD, USA) and B16-F10 mouse melanoma cells (ATCC No. CRL-6475) were incubated in Dulbecco's modified Eagle medium (DMEM; HyClone, UT, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; HyClone) and 1% (v/v) penicillin/streptomycin. ..

    Modification:

    Article Title: Bio-responsive adhesive antibody delivery platform for immunotherapy and preparation method thereof
    Article Snippet: .. MDA-MB-231 human breast cancer cells (ATCC No. HTB-26), luciferase/GFP double-labeled MDA-MB-231 cells (GeneCopoeia, Rockville, MD, USA) and B16-F10 mouse melanoma cells (ATCC No. CRL-6475) were incubated in Dulbecco's modified Eagle medium (DMEM; HyClone, UT, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; HyClone) and 1% (v/v) penicillin/streptomycin. ..

    shRNA:

    Article Title: Miro1 knockout inhibits mouse breast cancer tumorigenesis
    Article Snippet: .. MDA-MB-231 human breast cancer cells were transfected with shCtrl (OmicsLinkTM shRNA Expression Clone CSHCTR001-1-mU6; GeneCopoeia, Inc., Rockville, MD) or shMiro1 (OmicsLinkTM shRNA Expression Clone, HSH106063-mU6-a; GeneCopoeia, Inc., Rockville, MD) using the GeneJet plasmid miniprep kit (Thermo Scientific #K0502 #K0503) and the X-tremeGENE HP transfection reagent. ..

    Plasmid Preparation:

    Article Title: Miro1 knockout inhibits mouse breast cancer tumorigenesis
    Article Snippet: .. MDA-MB-231 human breast cancer cells were transfected with shCtrl (OmicsLinkTM shRNA Expression Clone CSHCTR001-1-mU6; GeneCopoeia, Inc., Rockville, MD) or shMiro1 (OmicsLinkTM shRNA Expression Clone, HSH106063-mU6-a; GeneCopoeia, Inc., Rockville, MD) using the GeneJet plasmid miniprep kit (Thermo Scientific #K0502 #K0503) and the X-tremeGENE HP transfection reagent. ..

    Stable Transfection:

    Article Title: Redirecting cytotoxic lymphocytes to breast cancer tumors via metabolite-sensing receptors
    Article Snippet: .. MDA-MB-231 cells stably expressing Cas9 were purchased from GeneCopoeia (SL515). .. Protospacer sequences of three NTC and three hEpCAM targeting sgRNAs were obtained from the Human CRISPR Knockout Pooled Library (GeCKO v2) and synthesized from IDT DNA.



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    Image Search Results


    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: GWAS, Marker, Single Cell, RNA Sequencing

    Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Journal: Communications Medicine

    Article Title: Autoantibody repertoire analysis in paraneoplastic pemphigus reveals novel targets linked to mucocutaneous blistering and bronchiolitis obliterans

    doi: 10.1038/s43856-025-01335-2

    Figure Lengend Snippet: a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Article Snippet: Single-cell expression data were obtained from the Human Protein Atlas.

    Techniques: Gene Expression, Expressing