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nnos expressing sh sy5y cells  (Genecopoeia)


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    Genecopoeia nnos expressing sh sy5y cells
    Nnos Expressing Sh Sy5y Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+SH-SY5Y+stably+expressing+CRISPR+Cas9%2C+randomly+inserted%2C+single+clone/pmc06202877-280-5-26
    Average 94 stars, based on 16 article reviews
    nnos expressing sh sy5y cells - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Regulation of the hippocampal translatome by Apoer2-ICD release.
    Article Snippet: For the TRAP constructs, the 3x-FLAG and Apoer2-ICD sequences were cloned into the pLVX-IRES-ZsGreen1 Vector (Clontech Cat#632187) and the ZsGreen sequence was replaced with the Cre-recombinase sequence. .. HEK-293T or SH-SY5Y cells were co-transfected with dual reporter construct containing a CMV-driven secreted alkaline phosphatase (SEAP) and a Reelin promoter-driven secreted Gaussia luciferase (GLuc) reporter construct (GeneCopoeia, cat. no:pEZX-GA01) and either GFP or an Apoer2-ICD (Apoer2-ICD:VP16 construct (±exon 19, Apoer2-ICD[+19/Δ19]±VP16). ..

    Article Title: Regulation of the hippocampal translatome by Apoer2-ICD release
    Article Snippet: For the TRAP constructs, the 3x-FLAG and Apoer2-ICD sequences were cloned into the pLVX-IRES-ZsGreen1 Vector (Clontech Cat#632187) and the ZsGreen sequence was replaced with the Cre-recombinase sequence. .. HEK-293T or SH-SY5Y cells were co-transfected with dual reporter construct containing a CMV-driven secreted alkaline phosphatase (SEAP) and a Reelin promoter-driven secreted Gaussia luciferase (GLuc) reporter construct (GeneCopoeia, cat. no:pEZX-GA01) and either GFP or an Apoer2-ICD (Apoer2-ICD:VP16 construct (±exon 19, Apoer2-ICD[+19/Δ19]±VP16). ..

    Article Title: Apoer2-ICD-dependent regulation of hippocampal ribosome mRNA loading
    Article Snippet: For the TRAP constructs, the 3x-FLAG and Apoer2-ICD sequences were cloned into the pLVX-IRES-ZsGreen1 Vector (Clontech Cat#632187) and the ZsGreen sequence was replaced with the Cre-recombinase sequence. .. Luciferase assay HEK-293T or SH-SY5Y cells were co-transfected with dual reporter construct containing a CMV-driven secreted alkaline phosphatase (SEAP) and a Reelin promoter-driven secreted Gaussia luciferase (GLuc) reporter construct (GeneCopoeia, cat. no:pEZX-GA01) and either GFP or an Apoer2-ICD (Apoer2-ICD:VP16 construct (± exon 19, Apoer2-ICD[+ 19/Δ19] ± VP16). ..

    Luciferase:

    Article Title: Regulation of the hippocampal translatome by Apoer2-ICD release.
    Article Snippet: For the TRAP constructs, the 3x-FLAG and Apoer2-ICD sequences were cloned into the pLVX-IRES-ZsGreen1 Vector (Clontech Cat#632187) and the ZsGreen sequence was replaced with the Cre-recombinase sequence. .. HEK-293T or SH-SY5Y cells were co-transfected with dual reporter construct containing a CMV-driven secreted alkaline phosphatase (SEAP) and a Reelin promoter-driven secreted Gaussia luciferase (GLuc) reporter construct (GeneCopoeia, cat. no:pEZX-GA01) and either GFP or an Apoer2-ICD (Apoer2-ICD:VP16 construct (±exon 19, Apoer2-ICD[+19/Δ19]±VP16). ..

    Article Title: Neuronal C/EBPβ/AEP pathway shortens life span via selective GABAnergic neuronal degeneration by FOXO repression
    Article Snippet: .. SH-SY5Y cells were seeded in 12-well plates and transfected with REST and FOXO1 Gaussia luciferase plasmid (HPRM46237 and HPRM44029, GeneCopoeia), treated with/without overexpressing plasmids or small interfering RNA. .. Forty-eight hours later, the cells were harvested in passive lysis buffer and analyzed using the Secrete-Pair Gaussia Luciferase Assay Kit (LF061, GeneCopoeia) on a microplate reader.

    Article Title: Regulation of the hippocampal translatome by Apoer2-ICD release
    Article Snippet: For the TRAP constructs, the 3x-FLAG and Apoer2-ICD sequences were cloned into the pLVX-IRES-ZsGreen1 Vector (Clontech Cat#632187) and the ZsGreen sequence was replaced with the Cre-recombinase sequence. .. HEK-293T or SH-SY5Y cells were co-transfected with dual reporter construct containing a CMV-driven secreted alkaline phosphatase (SEAP) and a Reelin promoter-driven secreted Gaussia luciferase (GLuc) reporter construct (GeneCopoeia, cat. no:pEZX-GA01) and either GFP or an Apoer2-ICD (Apoer2-ICD:VP16 construct (±exon 19, Apoer2-ICD[+19/Δ19]±VP16). ..

    Article Title: Apoer2-ICD-dependent regulation of hippocampal ribosome mRNA loading
    Article Snippet: For the TRAP constructs, the 3x-FLAG and Apoer2-ICD sequences were cloned into the pLVX-IRES-ZsGreen1 Vector (Clontech Cat#632187) and the ZsGreen sequence was replaced with the Cre-recombinase sequence. .. Luciferase assay HEK-293T or SH-SY5Y cells were co-transfected with dual reporter construct containing a CMV-driven secreted alkaline phosphatase (SEAP) and a Reelin promoter-driven secreted Gaussia luciferase (GLuc) reporter construct (GeneCopoeia, cat. no:pEZX-GA01) and either GFP or an Apoer2-ICD (Apoer2-ICD:VP16 construct (± exon 19, Apoer2-ICD[+ 19/Δ19] ± VP16). ..

    Transfection:

    Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)
    Article Snippet: .. For DNA transfection in differentiated SH-SY5Y cells, the cells were transfected with 750 ng/well of either GFP-tagged WT PINK1, or control vector (Genecopoeia, Rockville, MD) 2 days before MPP + treatment. ..

    Article Title: Neuronal C/EBPβ/AEP pathway shortens life span via selective GABAnergic neuronal degeneration by FOXO repression
    Article Snippet: .. SH-SY5Y cells were seeded in 12-well plates and transfected with REST and FOXO1 Gaussia luciferase plasmid (HPRM46237 and HPRM44029, GeneCopoeia), treated with/without overexpressing plasmids or small interfering RNA. .. Forty-eight hours later, the cells were harvested in passive lysis buffer and analyzed using the Secrete-Pair Gaussia Luciferase Assay Kit (LF061, GeneCopoeia) on a microplate reader.

    Article Title: Mitochondrial Proteostasis Requires Genes Encoded in a Neurodevelopmental Syndrome Locus
    Article Snippet: Neuroblastoma SH-SY5Y cells (ATCC, CRL2266; RRID:CVCL_0019) were grown in DMEM with 10% FBS and 100mg/ml penicillin and streptomycin at 37°C in 10% CO2. .. To produce stable cell lines, SH-SY5Y cells were transfected either with a control empty vector (GeneCopoeia, EX-NEG-Lv102) or ORF expression clone containing N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) or C terminally tagged MycDDK MRPL40 (Origene, RC202166). .. The stably transfected cell lines were selectively maintained in media containing DMEM supplemented with 10% FBS, 100mg/ml penicillin and streptomycin, and puromycin 2mg/ml (Invitrogen, A1113803) or neomycin 0.2mg/ml (Hyclone, SV30068), respectively, at 37°C in 10% CO2.

    Control:

    Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)
    Article Snippet: .. For DNA transfection in differentiated SH-SY5Y cells, the cells were transfected with 750 ng/well of either GFP-tagged WT PINK1, or control vector (Genecopoeia, Rockville, MD) 2 days before MPP + treatment. ..

    Article Title: Mitochondrial Proteostasis Requires Genes Encoded in a Neurodevelopmental Syndrome Locus
    Article Snippet: Neuroblastoma SH-SY5Y cells (ATCC, CRL2266; RRID:CVCL_0019) were grown in DMEM with 10% FBS and 100mg/ml penicillin and streptomycin at 37°C in 10% CO2. .. To produce stable cell lines, SH-SY5Y cells were transfected either with a control empty vector (GeneCopoeia, EX-NEG-Lv102) or ORF expression clone containing N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) or C terminally tagged MycDDK MRPL40 (Origene, RC202166). .. The stably transfected cell lines were selectively maintained in media containing DMEM supplemented with 10% FBS, 100mg/ml penicillin and streptomycin, and puromycin 2mg/ml (Invitrogen, A1113803) or neomycin 0.2mg/ml (Hyclone, SV30068), respectively, at 37°C in 10% CO2.

    Plasmid Preparation:

    Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)
    Article Snippet: .. For DNA transfection in differentiated SH-SY5Y cells, the cells were transfected with 750 ng/well of either GFP-tagged WT PINK1, or control vector (Genecopoeia, Rockville, MD) 2 days before MPP + treatment. ..

    Article Title: Neuronal C/EBPβ/AEP pathway shortens life span via selective GABAnergic neuronal degeneration by FOXO repression
    Article Snippet: .. SH-SY5Y cells were seeded in 12-well plates and transfected with REST and FOXO1 Gaussia luciferase plasmid (HPRM46237 and HPRM44029, GeneCopoeia), treated with/without overexpressing plasmids or small interfering RNA. .. Forty-eight hours later, the cells were harvested in passive lysis buffer and analyzed using the Secrete-Pair Gaussia Luciferase Assay Kit (LF061, GeneCopoeia) on a microplate reader.

    Small Interfering RNA:

    Article Title: Neuronal C/EBPβ/AEP pathway shortens life span via selective GABAnergic neuronal degeneration by FOXO repression
    Article Snippet: .. SH-SY5Y cells were seeded in 12-well plates and transfected with REST and FOXO1 Gaussia luciferase plasmid (HPRM46237 and HPRM44029, GeneCopoeia), treated with/without overexpressing plasmids or small interfering RNA. .. Forty-eight hours later, the cells were harvested in passive lysis buffer and analyzed using the Secrete-Pair Gaussia Luciferase Assay Kit (LF061, GeneCopoeia) on a microplate reader.

    Stable Transfection:

    Article Title: Mitochondrial Proteostasis Requires Genes Encoded in a Neurodevelopmental Syndrome Locus
    Article Snippet: Neuroblastoma SH-SY5Y cells (ATCC, CRL2266; RRID:CVCL_0019) were grown in DMEM with 10% FBS and 100mg/ml penicillin and streptomycin at 37°C in 10% CO2. .. To produce stable cell lines, SH-SY5Y cells were transfected either with a control empty vector (GeneCopoeia, EX-NEG-Lv102) or ORF expression clone containing N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) or C terminally tagged MycDDK MRPL40 (Origene, RC202166). .. The stably transfected cell lines were selectively maintained in media containing DMEM supplemented with 10% FBS, 100mg/ml penicillin and streptomycin, and puromycin 2mg/ml (Invitrogen, A1113803) or neomycin 0.2mg/ml (Hyclone, SV30068), respectively, at 37°C in 10% CO2.

    Expressing:

    Article Title: Mitochondrial Proteostasis Requires Genes Encoded in a Neurodevelopmental Syndrome Locus
    Article Snippet: Neuroblastoma SH-SY5Y cells (ATCC, CRL2266; RRID:CVCL_0019) were grown in DMEM with 10% FBS and 100mg/ml penicillin and streptomycin at 37°C in 10% CO2. .. To produce stable cell lines, SH-SY5Y cells were transfected either with a control empty vector (GeneCopoeia, EX-NEG-Lv102) or ORF expression clone containing N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) or C terminally tagged MycDDK MRPL40 (Origene, RC202166). .. The stably transfected cell lines were selectively maintained in media containing DMEM supplemented with 10% FBS, 100mg/ml penicillin and streptomycin, and puromycin 2mg/ml (Invitrogen, A1113803) or neomycin 0.2mg/ml (Hyclone, SV30068), respectively, at 37°C in 10% CO2.

    Modification:

    Article Title: Suppression of SAMSN1 contributes to neuroprotection in neonatal rats suffering from hypoxic–ischemic encephalopathy injury
    Article Snippet: .. SY5Y cell lines (GeneCopoeia Company) were cultivated in flasks with a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) (1.5 g/L sodium bicarbonate, 2 mM l ‐glutamine, 1 mM sodium pyruvate, and 0.1 mM nonessential amino acids), Ham's F12 medium, and 10% supplemental fetal bovine serum (FBS) at 37°C with 5% CO 2 . ..

    Article Title: Suppression of SAMSN1 contributes to neuroprotection in neonatal rats suffering from hypoxic-ischemic encephalopathy injury.
    Article Snippet: .. SY5Y cell lines (GeneCopoeia Company) were cultivated in flasks with a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) (1.5 g/L sodium bicarbonate, 2mM L‐ glutamine, 1mM sodium pyruvate, and 0.1mM nonessential amino acids), Ham's F12 medium, and 10% supplemental fetal bovine serum (FBS) at 37°C with 5% CO2. ..



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    Image Search Results


    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: GWAS, Marker, Single Cell, RNA Sequencing

    Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Journal: Communications Medicine

    Article Title: Autoantibody repertoire analysis in paraneoplastic pemphigus reveals novel targets linked to mucocutaneous blistering and bronchiolitis obliterans

    doi: 10.1038/s43856-025-01335-2

    Figure Lengend Snippet: a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Article Snippet: Single-cell expression data were obtained from the Human Protein Atlas.

    Techniques: Gene Expression, Expressing