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t47d  (Genecopoeia)


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    Genecopoeia t47d
    T47d, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+T47D+stably+expressing+CRISPR+Cas9%2C+AAVS1+inserted%2C+single+clone/pm40186268-122-4-1
    Average 94 stars, based on 5 article reviews
    t47d - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Cell Division Cycle Associated 8 Is a Key Regulator of Tamoxifen Resistance in Breast Cancer
    Article Snippet: .. To establish stable cell lines, T47D and MCF-7 cells were infected with lentivirus carrying shRNA targeting CDCA8 (shCDCA8) or negative control precursor (Genecopoeia, Rockville, USA). ..

    Article Title: Breast cancer cell-secreted miR-199b-5p hijacks neurometabolic coupling to promote brain metastasis
    Article Snippet: .. MDA-MB-231 and T47D cells were engineered to stably overexpress hsa-mir-199b (MDA-231/199b and T47D/199b), hsa-mir-211 (MDA-231/211), or the control vector (MDA-231/ctrl and T47D/ctrl) using lentiviral constructs HmiR0152-MR03, HmiR0250-MR03, or pEZX-MR03 (vector) from GeneCopoeia (Rockville, MD). ..

    Infection:

    Article Title: Cell Division Cycle Associated 8 Is a Key Regulator of Tamoxifen Resistance in Breast Cancer
    Article Snippet: .. To establish stable cell lines, T47D and MCF-7 cells were infected with lentivirus carrying shRNA targeting CDCA8 (shCDCA8) or negative control precursor (Genecopoeia, Rockville, USA). ..

    shRNA:

    Article Title: Cell Division Cycle Associated 8 Is a Key Regulator of Tamoxifen Resistance in Breast Cancer
    Article Snippet: .. To establish stable cell lines, T47D and MCF-7 cells were infected with lentivirus carrying shRNA targeting CDCA8 (shCDCA8) or negative control precursor (Genecopoeia, Rockville, USA). ..

    Negative Control:

    Article Title: Cell Division Cycle Associated 8 Is a Key Regulator of Tamoxifen Resistance in Breast Cancer
    Article Snippet: .. To establish stable cell lines, T47D and MCF-7 cells were infected with lentivirus carrying shRNA targeting CDCA8 (shCDCA8) or negative control precursor (Genecopoeia, Rockville, USA). ..

    Expressing:

    Article Title: PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER + Breast Cancer
    Article Snippet: .. T47D, T47D Palbo-R, MCF7 EDR, and MCF7 EDR Palbo-R cell lines expressing Gaussia luciferase (GLuc) under the control of the human PKMYT1 promoter were created by lentiviral transduction using a PKMYT1 promoter-GLuc reporter obtained from GeneCopoeia (cat. # HPRM40000-LvPG04). .. For lentiviral transduction of MCF7 EDR and MCF EDR Palbo-R cells, nontargeting shRNA and two different TP53 -targeting shRNAs in the pGIPZ lentiviral vector were obtained from Horizon Discovery (cat. # RHS4346, RHS4430-200296439, and RHS4430-200289946, respectively).

    Luciferase:

    Article Title: PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER + Breast Cancer
    Article Snippet: .. T47D, T47D Palbo-R, MCF7 EDR, and MCF7 EDR Palbo-R cell lines expressing Gaussia luciferase (GLuc) under the control of the human PKMYT1 promoter were created by lentiviral transduction using a PKMYT1 promoter-GLuc reporter obtained from GeneCopoeia (cat. # HPRM40000-LvPG04). .. For lentiviral transduction of MCF7 EDR and MCF EDR Palbo-R cells, nontargeting shRNA and two different TP53 -targeting shRNAs in the pGIPZ lentiviral vector were obtained from Horizon Discovery (cat. # RHS4346, RHS4430-200296439, and RHS4430-200289946, respectively).

    Control:

    Article Title: PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER + Breast Cancer
    Article Snippet: .. T47D, T47D Palbo-R, MCF7 EDR, and MCF7 EDR Palbo-R cell lines expressing Gaussia luciferase (GLuc) under the control of the human PKMYT1 promoter were created by lentiviral transduction using a PKMYT1 promoter-GLuc reporter obtained from GeneCopoeia (cat. # HPRM40000-LvPG04). .. For lentiviral transduction of MCF7 EDR and MCF EDR Palbo-R cells, nontargeting shRNA and two different TP53 -targeting shRNAs in the pGIPZ lentiviral vector were obtained from Horizon Discovery (cat. # RHS4346, RHS4430-200296439, and RHS4430-200289946, respectively).

    Article Title: Breast cancer cell-secreted miR-199b-5p hijacks neurometabolic coupling to promote brain metastasis
    Article Snippet: .. MDA-MB-231 and T47D cells were engineered to stably overexpress hsa-mir-199b (MDA-231/199b and T47D/199b), hsa-mir-211 (MDA-231/211), or the control vector (MDA-231/ctrl and T47D/ctrl) using lentiviral constructs HmiR0152-MR03, HmiR0250-MR03, or pEZX-MR03 (vector) from GeneCopoeia (Rockville, MD). ..

    Transduction:

    Article Title: PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER + Breast Cancer
    Article Snippet: .. T47D, T47D Palbo-R, MCF7 EDR, and MCF7 EDR Palbo-R cell lines expressing Gaussia luciferase (GLuc) under the control of the human PKMYT1 promoter were created by lentiviral transduction using a PKMYT1 promoter-GLuc reporter obtained from GeneCopoeia (cat. # HPRM40000-LvPG04). .. For lentiviral transduction of MCF7 EDR and MCF EDR Palbo-R cells, nontargeting shRNA and two different TP53 -targeting shRNAs in the pGIPZ lentiviral vector were obtained from Horizon Discovery (cat. # RHS4346, RHS4430-200296439, and RHS4430-200289946, respectively).

    Multiple Displacement Amplification:

    Article Title: Breast cancer cell-secreted miR-199b-5p hijacks neurometabolic coupling to promote brain metastasis
    Article Snippet: .. MDA-MB-231 and T47D cells were engineered to stably overexpress hsa-mir-199b (MDA-231/199b and T47D/199b), hsa-mir-211 (MDA-231/211), or the control vector (MDA-231/ctrl and T47D/ctrl) using lentiviral constructs HmiR0152-MR03, HmiR0250-MR03, or pEZX-MR03 (vector) from GeneCopoeia (Rockville, MD). ..

    Plasmid Preparation:

    Article Title: Breast cancer cell-secreted miR-199b-5p hijacks neurometabolic coupling to promote brain metastasis
    Article Snippet: .. MDA-MB-231 and T47D cells were engineered to stably overexpress hsa-mir-199b (MDA-231/199b and T47D/199b), hsa-mir-211 (MDA-231/211), or the control vector (MDA-231/ctrl and T47D/ctrl) using lentiviral constructs HmiR0152-MR03, HmiR0250-MR03, or pEZX-MR03 (vector) from GeneCopoeia (Rockville, MD). ..

    Construct:

    Article Title: Breast cancer cell-secreted miR-199b-5p hijacks neurometabolic coupling to promote brain metastasis
    Article Snippet: .. MDA-MB-231 and T47D cells were engineered to stably overexpress hsa-mir-199b (MDA-231/199b and T47D/199b), hsa-mir-211 (MDA-231/211), or the control vector (MDA-231/ctrl and T47D/ctrl) using lentiviral constructs HmiR0152-MR03, HmiR0250-MR03, or pEZX-MR03 (vector) from GeneCopoeia (Rockville, MD). ..

    Cell Culture:

    Article Title: Gene expression analysis in circulating tumour cells to determine resistance to CDK4/6 inhibitors plus endocrine therapy in HR + /HER2- metastatic breast cancer patients
    Article Snippet: The positive controls were synthetic double-stranded DNA fragments, called gBlock ® Gene Fragments (Bio-Rad), which contained 10 or 50 copies of the selected mutation. .. MCF7 (GeneCopoeia, Inc.) and T47D (ATCC) cells were cultured in DMEM (Sigma Aldrich) and RPMI 1640 (Sigma Aldrich), respectively, supplemented with 10% FBS (Sigma Aldrich) and 1% penicillin‒streptomycin solution (Sigma Aldrich) at 37 °C in a humidified atmosphere with 5% CO 2 . .. Both cell lines were cultured with CDK4/6i (palbociclib, ribociclib), obtained from Selleckchem to generate resistant cell lines (Supplementary material).

    Article Title: Gene expression analysis in circulating tumour cells to determine resistance to CDK4/6 inhibitors plus endocrine therapy in HR + /HER2- metastatic breast cancer patients.
    Article Snippet: The positive controls were synthetic double-stranded DNA fragments, called gBlock® Gene Fragments (Bio-Rad), which contained 10 or 50 copies of the selected mutation. .. MCF7 (GeneCopoeia, Inc.) and T47D (ATCC) cells were cultured in DMEM (Sigma Aldrich) and RPMI 1640 (Sigma Aldrich), respectively, supplemented with 10% FBS (Sigma Aldrich) and 1% penicillin‒streptomycin solution (Sigma Aldrich) at 37 °C in a humidified atmosphere with 5% CO2. .. Both cell lines were cultured with CDK4/6i (palbociclib, ribociclib), obtained from Selleckchem to generate resistant cell lines (Supplementary material).

    Article Title: Hormonal Modulation of ESR1 Mutant Metastasis
    Article Snippet: Corning Collagen IV was from Thermo Fisher Scientific (Waltham, MA). .. LV103 or LV111 plasmids were used to make lentivirus according to manufacturer’s (Genecopoeia) instructions T47D (Parental and YS53) and MCF-7 (BK parental, SF parental, YS1, YS30 and DG2) cells were cultured in RPMI 1640 and MEM medium respectively containing 10% fetal bovine serum, 0.1 mM nonessential amino acids, 100 IU/mL penicillin, and 100 μg/mL streptomycin. ..



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    Image Search Results


    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: GWAS, Marker, Single Cell, RNA Sequencing

    Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Journal: Communications Medicine

    Article Title: Autoantibody repertoire analysis in paraneoplastic pemphigus reveals novel targets linked to mucocutaneous blistering and bronchiolitis obliterans

    doi: 10.1038/s43856-025-01335-2

    Figure Lengend Snippet: a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Article Snippet: Single-cell expression data were obtained from the Human Protein Atlas.

    Techniques: Gene Expression, Expressing