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luciferase stable nci h1299 cells  (Genecopoeia)


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    Genecopoeia luciferase stable nci h1299 cells
    Luciferase Stable Nci H1299 Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+NCI-H1299+stably+expressing+Cas9%2C+randomly+inserted%2C+single+clone/pm32705796-253-7-12
    Average 94 stars, based on 3 article reviews
    luciferase stable nci h1299 cells - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Luciferase:

    Article Title: One cell, one drop, one click: hybrid microfluidic mammalian single-cell isolation
    Article Snippet: .. Human lung squamous cell carcinoma dual-labeled (eGFP, Luciferase) stable NCI-H1299 (SCL-C01-HLG; Genecopoeia, Inc, Rockville, MD) cells were grown in RPMI-1640 containing 10% fetal bovine serum without antibiotics at 37°C with 5% CO 2 . ..

    Article Title: One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.
    Article Snippet: .. Human lung squamous cell carcinoma dual-labeled (eGFP, Luciferase) stable NCI-H1299 cells (SCL-C01-HLG; Genecopoeia, Inc, Rockville, MD) were grown in RPMI-1640 containing 10% FBS without antibiotics at 37 °C with 5% CO2. ..

    Stable Transfection:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: A549 stably expressing Cas9 from AAVS1 site , Genecopeia , Cat#SL504. .. HEK-293T stably expressing cas9-HF1 from AAVS1 site , Genecopeia , Cat#SL533. .. Lenti-XTM 293T cell Line , Takara Bio , Cat#632180.

    Article Title: Metastasis suppressor 1 (MTSS1) expression is associated with reduced in-vivo metastasis and enhanced patient survival in lung adenocarcinoma.
    Article Snippet: Metastasis suppressor 1 (MTSS1) has been shown to be a metastasis suppressor in a number of cancers.. However, its role in lung adenocarcinoma is largely unknown.. To evaluate the significance of MTSS1 expression on lung adenocarcinoma metastatic properties, the gain or loss of MTSS1 in in vivo and in vitro experiments were employed.

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Expressing:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: A549 stably expressing Cas9 from AAVS1 site , Genecopeia , Cat#SL504. .. HEK-293T stably expressing cas9-HF1 from AAVS1 site , Genecopeia , Cat#SL533. .. Lenti-XTM 293T cell Line , Takara Bio , Cat#632180.

    Article Title: Metastasis suppressor 1 (MTSS1) expression is associated with reduced in-vivo metastasis and enhanced patient survival in lung adenocarcinoma.
    Article Snippet: Metastasis suppressor 1 (MTSS1) has been shown to be a metastasis suppressor in a number of cancers.. However, its role in lung adenocarcinoma is largely unknown.. To evaluate the significance of MTSS1 expression on lung adenocarcinoma metastatic properties, the gain or loss of MTSS1 in in vivo and in vitro experiments were employed.

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Plasmid Preparation:

    Article Title: Metastasis suppressor 1 (MTSS1) expression is associated with reduced in-vivo metastasis and enhanced patient survival in lung adenocarcinoma.
    Article Snippet: Metastasis suppressor 1 (MTSS1) has been shown to be a metastasis suppressor in a number of cancers.. However, its role in lung adenocarcinoma is largely unknown.. To evaluate the significance of MTSS1 expression on lung adenocarcinoma metastatic properties, the gain or loss of MTSS1 in in vivo and in vitro experiments were employed.

    Transfection:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    CRISPR:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Recombinant:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Software:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Variant Assay:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Activity Assay:

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells.
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..

    Article Title: Computationally guided high-throughput engineering of an anti-CRISPR protein for precise genome editing in human cells
    Article Snippet: Le Cong Lab N/A Chemicals, peptides, and recombinant proteins PEG-it virus precipitation solution System Biosciences Cat#LV810A-1 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat#1081058 Puromycin dihydrochloride Thermo Fisher Cat#A1113803 Lipofectamine 3000 Thermo Fisher Cat#L3000001 Critical commercial assays InvitrogenTM NeonTM Transfection System 100 mL Kit Thermo Fisher Cat#MPK10096 MiSeq Reagent Kit Illumina Cat#MS-102-2002 NextSeq Reagent Kit Illumina Cat#20050264 Deposited data Primary screening across AcrIIA4 variants This study Zenodo: https://doi.org/10.5281/ zenodo.13621371 Experimental models: cell lines A549 stably expressing Cas9 from AAVS1 site Genecopeia CatSL504 HEK-293T stably expressing cas9-HF1 from AAVS1 site Genecopeia Cat#SL533 Lenti-XTM 293T cell Line Takara Bio Cat#632180 Oligonucleotides Alt-R CRISPR-Cas9 crRNA Integrated DNA Technologies See Table S1 Custom primers for editing analyses Integrated DNA Technologies See Table S1 Recombinant DNA pAG002 This study N/A Software and algorithms Cutadapt Martin62 https://cutadapt.re adthedocs.io/en/st able/installation.ht ml FLASh Magoĉ et al.63 https://sourceforge .net/projects/flashp age/ Needleman-Wunsch algorithm Rice et al.64 https://github.com/noporpoise/seq-align CRISPResso2 Clement et al.65 https://github.com/pinellolab/CRISPR esso2 Jackhmmer algorithm Finn et al.66 http://hmmer.org/ DeepSequence Riesselman et al.58 https://github.com/debbiemarkslab/ D eepSequence Custom python scripts to demultiplex pooled variant data and to quantify editing activity This study https://doi.org/10.5281/zenodo.13621371 ..



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    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: GWAS, Marker, Single Cell, RNA Sequencing

    Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

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    Article Title: Autoantibody repertoire analysis in paraneoplastic pemphigus reveals novel targets linked to mucocutaneous blistering and bronchiolitis obliterans

    doi: 10.1038/s43856-025-01335-2

    Figure Lengend Snippet: a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Article Snippet: Single-cell expression data were obtained from the Human Protein Atlas.

    Techniques: Gene Expression, Expressing