Review



lentiviral stable transduction du145 cells  (Genecopoeia)


Bioz Verified Symbol Genecopoeia is a verified supplier
Bioz Manufacturer Symbol Genecopoeia manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Genecopoeia lentiviral stable transduction du145 cells
    Lentiviral Stable Transduction Du145 Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/Human+cell+line+DU145+stably+expressing+CRISPR+Cas9%2C+randomly+inserted%2C+single+clone/pm32618200-36-3-25
    Average 94 stars, based on 7 article reviews
    lentiviral stable transduction du145 cells - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Paradoxical Role of Glypican-1 in Prostate Cancer Cell and Tumor Growth
    Article Snippet: .. A. DU-145 cells were transfected with GPC-1 shRNA (Genecopoeia, Rockville, MD) prior to analysis of GPC-1 expression using immunoblot analysis. ..

    Article Title: Spatial distribution of tumor-associated macrophages in an orthotopic prostate cancer mouse model
    Article Snippet: Media was supplemented with 10% fetal bovine serum and 1% penicillin (Gibco, ThermoFisher, Waltham, MA, United States). .. DU145 cells were transfected with a pCMV lentivirus containing mCherry and D-luciferase (LP466-025) markers along with a puromycin gene (GeneCopoeia, Rockville, MD, United States). .. Infected cells were selected with puromycin (P8833, Sigma Aldrich, St. Louis, MO, United States) for puromycin resistance and were cultured according to ATCC guidelines.

    Article Title: LncRNA HORAS5 promotes taxane resistance in castration-resistant prostate cancer via a BCL2A1-dependent mechanism.
    Article Snippet: .. HORAS5 overexpression with lentiviral stable transduction DU145 cells that express HORAS5 at undetectable levels were stably transfected with a lentivirus-derived particle that induced HORAS5 overexpression (Genecopoeia, MD, USA, Cat# LPP-GS266B-Lv105-050). ..

    Gel Permeation Chromatography:

    Article Title: Paradoxical Role of Glypican-1 in Prostate Cancer Cell and Tumor Growth
    Article Snippet: .. A. DU-145 cells were transfected with GPC-1 shRNA (Genecopoeia, Rockville, MD) prior to analysis of GPC-1 expression using immunoblot analysis. ..

    shRNA:

    Article Title: Paradoxical Role of Glypican-1 in Prostate Cancer Cell and Tumor Growth
    Article Snippet: .. A. DU-145 cells were transfected with GPC-1 shRNA (Genecopoeia, Rockville, MD) prior to analysis of GPC-1 expression using immunoblot analysis. ..

    Expressing:

    Article Title: Paradoxical Role of Glypican-1 in Prostate Cancer Cell and Tumor Growth
    Article Snippet: .. A. DU-145 cells were transfected with GPC-1 shRNA (Genecopoeia, Rockville, MD) prior to analysis of GPC-1 expression using immunoblot analysis. ..

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth.
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Western Blot:

    Article Title: Paradoxical Role of Glypican-1 in Prostate Cancer Cell and Tumor Growth
    Article Snippet: .. A. DU-145 cells were transfected with GPC-1 shRNA (Genecopoeia, Rockville, MD) prior to analysis of GPC-1 expression using immunoblot analysis. ..

    Plasmid Preparation:

    Article Title: miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis.
    Article Snippet: Accepted Manuscript miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis Rihan El Bezawy, Denis Cominetti, Nicola Fenderico, Valentina Zuco, Giovanni Luca Beretta, Matteo Dugo, Noemi Arrighetti, Claudio Stucchi, Tiziana Rancati, Riccardo Valdagni, Nadia Zaffaroni, Paolo Gandellini PII: S0304-3835(17)30150-7 DOI: 10.1016/j.canlet.2017.02.033 Reference: CAN 13261 To appear in: Cancer Letters Received Date: 9 January 2017 Revised Date: 24 February 2017 Accepted Date: 24 February 2017 Please cite this article as: R. El Bezawy, D. Cominetti, N. Fenderico, V. Zuco, G.L.. Beretta, M. Dugo, N. Arrighetti, C. Stucchi, T. Rancati, R. Valdagni, N. Zaffaroni, P. Gandellini, miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis, Cancer Letters (2017), doi: 10.1016/j.canlet.2017.02.033.. This is a PDF file of an unedited manuscript that has been accepted for publication.

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth.
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Sequencing:

    Article Title: miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis.
    Article Snippet: Accepted Manuscript miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis Rihan El Bezawy, Denis Cominetti, Nicola Fenderico, Valentina Zuco, Giovanni Luca Beretta, Matteo Dugo, Noemi Arrighetti, Claudio Stucchi, Tiziana Rancati, Riccardo Valdagni, Nadia Zaffaroni, Paolo Gandellini PII: S0304-3835(17)30150-7 DOI: 10.1016/j.canlet.2017.02.033 Reference: CAN 13261 To appear in: Cancer Letters Received Date: 9 January 2017 Revised Date: 24 February 2017 Accepted Date: 24 February 2017 Please cite this article as: R. El Bezawy, D. Cominetti, N. Fenderico, V. Zuco, G.L.. Beretta, M. Dugo, N. Arrighetti, C. Stucchi, T. Rancati, R. Valdagni, N. Zaffaroni, P. Gandellini, miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis, Cancer Letters (2017), doi: 10.1016/j.canlet.2017.02.033.. This is a PDF file of an unedited manuscript that has been accepted for publication.

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth.
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Clone Assay:

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth.
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Article Title: Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth
    Article Snippet: .. A lentiviral vector bearing the precursor nc886 or a scrambled sequence of the same length, both cloned downstream of the CMV promoter (miExpress precursor expression clones, pEZX-MR02, GeneCopoeia) were transduced in DU145 and LNCaP. ..

    Selection:

    Article Title: The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression
    Article Snippet: .. Lentiviral GFP-tagged ERF (GeneCopoeia, EX-S0501-Lv122) was used to develop DU-145 ERF cells with puromycin (1 μg/ml) as a selection marker. ..

    Article Title: The CIC-ERF co-deletion underlies fusion independent activation of ETS family member, ETV1, to drive prostate cancer progression
    Article Snippet: .. Lentiviral GFP-tagged ERF (GeneCopoeia, EX-S0501-Lv122) was used to develop DU-145 ERF cells with puromycin (1μg/ml) as a selection marker. ..

    Marker:

    Article Title: The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression
    Article Snippet: .. Lentiviral GFP-tagged ERF (GeneCopoeia, EX-S0501-Lv122) was used to develop DU-145 ERF cells with puromycin (1 μg/ml) as a selection marker. ..

    Article Title: The CIC-ERF co-deletion underlies fusion independent activation of ETS family member, ETV1, to drive prostate cancer progression
    Article Snippet: .. Lentiviral GFP-tagged ERF (GeneCopoeia, EX-S0501-Lv122) was used to develop DU-145 ERF cells with puromycin (1μg/ml) as a selection marker. ..

    Over Expression:

    Article Title: LncRNA HORAS5 promotes taxane resistance in castration-resistant prostate cancer via a BCL2A1-dependent mechanism.
    Article Snippet: .. HORAS5 overexpression with lentiviral stable transduction DU145 cells that express HORAS5 at undetectable levels were stably transfected with a lentivirus-derived particle that induced HORAS5 overexpression (Genecopoeia, MD, USA, Cat# LPP-GS266B-Lv105-050). ..

    Transduction:

    Article Title: LncRNA HORAS5 promotes taxane resistance in castration-resistant prostate cancer via a BCL2A1-dependent mechanism.
    Article Snippet: .. HORAS5 overexpression with lentiviral stable transduction DU145 cells that express HORAS5 at undetectable levels were stably transfected with a lentivirus-derived particle that induced HORAS5 overexpression (Genecopoeia, MD, USA, Cat# LPP-GS266B-Lv105-050). ..

    Stable Transfection:

    Article Title: LncRNA HORAS5 promotes taxane resistance in castration-resistant prostate cancer via a BCL2A1-dependent mechanism.
    Article Snippet: .. HORAS5 overexpression with lentiviral stable transduction DU145 cells that express HORAS5 at undetectable levels were stably transfected with a lentivirus-derived particle that induced HORAS5 overexpression (Genecopoeia, MD, USA, Cat# LPP-GS266B-Lv105-050). ..



    Similar Products

    single cell rna sequencing gene expression data  (Broad Clinical Labs)
    96
    Broad Clinical Labs single cell rna sequencing gene expression data
    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; <t>scRNA-seq</t> <t>single-cell</t> <t>RNA</t> <t>sequencing</t>
    Single Cell Rna Sequencing Gene Expression Data, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/RNA+Sequencing/pmc13096280-240-1-12
    Average 96 stars, based on 1 article reviews
    single cell rna sequencing gene expression data - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    single cell expression data  (Human Protein Atlas)
    86
    Human Protein Atlas single cell expression data
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Single Cell Expression Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/data+rna+seq/pmc12873347-132-0-7
    Average 86 stars, based on 1 article reviews
    single cell expression data - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    single cell rna expression data  (Human Protein Atlas)
    86
    Human Protein Atlas single cell rna expression data
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Single Cell Rna Expression Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/data+rna+seq/pmc12882627-172-8-15
    Average 86 stars, based on 1 article reviews
    single cell rna expression data - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    single cell gene expression data  (Human Protein Atlas)
    86
    Human Protein Atlas single cell gene expression data
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Single Cell Gene Expression Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/data+rna+seq/pmc12540724-132-30-27
    Average 86 stars, based on 1 article reviews
    single cell gene expression data - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    single cell expression profile data  (Muris Inc)
    86
    Muris Inc single cell expression profile data
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Single Cell Expression Profile Data, supplied by Muris Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/cell+cell+czbiohub+expression+mouse+muris+org+sf+single+single+tabula+vignette/pmc12659644-207-0-19
    Average 86 stars, based on 1 article reviews
    single cell expression profile data - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    gene expression specificity analysis single cell transcriptomic data  (Human Protein Atlas)
    86
    Human Protein Atlas gene expression specificity analysis single cell transcriptomic data
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Gene Expression Specificity Analysis Single Cell Transcriptomic Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/data+rna+seq/pm40830700-267-0-9
    Average 86 stars, based on 1 article reviews
    gene expression specificity analysis single cell transcriptomic data - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    data exploration, quality control and testing in single-cell qpcr-based gene expression experiments  (McDavid Inc)
    90
    McDavid Inc data exploration, quality control and testing in single-cell qpcr-based gene expression experiments
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Data Exploration, Quality Control And Testing In Single Cell Qpcr Based Gene Expression Experiments, supplied by McDavid Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/quantitation+of+gene+expression/pm40622896-542-20-0
    Average 90 stars, based on 1 article reviews
    data exploration, quality control and testing in single-cell qpcr-based gene expression experiments - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    single nuclei multiome atac gene expression data cell ranger arc  (10X Genomics)
    86
    10X Genomics single nuclei multiome atac gene expression data cell ranger arc
    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing <t>normalized</t> <t>single-cell</t> transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.
    Single Nuclei Multiome Atac Gene Expression Data Cell Ranger Arc, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+expression+data/isolation+nuclei/pm40593578-240-8-21
    Average 86 stars, based on 1 article reviews
    single nuclei multiome atac gene expression data cell ranger arc - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Flowchart Overview of this MR-based study. LD linkage disequilibrium, ρ-HESS Heritability Estimation from Summary Statistics, GNOVA Genetic covariance analyser, MTAG Multi-Trait Analysis of GWAS, CPASSOC Cross Phenotype Association, GWAS Genome-wide Association Study, MR Mendelian Randomization, GSMR Generalized summary-data-based Mendelian randomization, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, SMR Summary-databased Mendelian randomization, Phe-MR phenotypes, MR BMI body mass index, LUSC squamous cell lung cancer; scRNA-seq single-cell RNA sequencing

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: GWAS, Marker, Single Cell, RNA Sequencing

    Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Journal: Discover Oncology

    Article Title: Genetic evidence supporting obesity as a risk factor for lung squamous cell carcinoma and the identification of MFAP1 as a shared genetic target

    doi: 10.1007/s12672-026-04793-9

    Figure Lengend Snippet: Tissue- and cell type–specific enrichment of SNP heritability for BMI and LUSC. A Tissue-level SNP heritability enrichment for BMI across 53 GTEx tissues estimated using LDSC-SEG. The x-axis shows tissues grouped by organ system, and the y-axis shows the estimated enrichment coefficient (log-scaled or standardized). Tissues surpassing the significance threshold (FDR-adjusted P < 0.05) are highlighted. B Tissue-level SNP heritability enrichment for LUSC using the same LDSC-SEG framework. The x-axis indicates GTEx tissues and the y-axis indicates enrichment coefficients, as in panel ( A ). Tissues with significant enrichment (FDR-adjusted P < 0.05) are labelled, illustrating the overlap and differences in tissue-specific genetic architecture between BMI and LUSC. C – G Cell type–specific SNP heritability enrichment for BMI and LUSC across single-cell RNA sequencing datasets from brain, ileum, heart, and colon, estimated using MAGMA-based cell typing. For each tissue, the left panel shows enrichment for BMI and the right panel shows enrichment for LUSC. The y-axis lists cell types (for example, neurons, epithelial cells, eosinophils, CD8⁺ T cells), and the x-axis shows –log₁₀(P) values for the association between cell type–specific expression and SNP heritability. Cell types with P < 0.05 are indicated in red, highlighting those most strongly enriched for the genetic signal of BMI and/or LUSC. BMI body mass index, LUSC lung squamous cell carcinoma, SNP single nucleotide polymorphism, LDSC-SEG linkage disequilibrium score regression applied to specifically expressed genes, MAGMA Multi-marker Analysis of GenoMic Annotation, scRNA-seq single-cell RNA sequencing, GTEx Genotype-Tissue Expression

    Article Snippet: The Single-cell RNA sequencing gene expression data were available from https://www.ncbi.nlm.nih.gov/geo/ and [https://singlecell.broadinstitute.org/single_cell](https:/www.singlecell.broadinstitute.org/single_cell) .

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Journal: Communications Medicine

    Article Title: Autoantibody repertoire analysis in paraneoplastic pemphigus reveals novel targets linked to mucocutaneous blistering and bronchiolitis obliterans

    doi: 10.1038/s43856-025-01335-2

    Figure Lengend Snippet: a Gene expression levels (Transcripts Per Million, TPM) of major autoantibody targets identified in paraneoplastic pemphigus, showing a marked enrichment in the skin and other squamous epithelia (data from the GTEx database, v6p.v1.1.8). Each bar comprises stacked boxes, with the width of each box representing the interquartile range of gene expression in the corresponding tissue. b – d Heatmaps showing normalized single-cell transcript expression levels (nTPM), summarized per gene and cell type cluster for selected tissues, highlighting SERPINB3 expression in bronchiolar cells. Single-cell expression data were obtained from the Human Protein Atlas.

    Article Snippet: Single-cell expression data were obtained from the Human Protein Atlas.

    Techniques: Gene Expression, Expressing