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Bioer Technology Co Ltd simply p virus rna extraction kit
Simply P Virus Rna Extraction Kit, supplied by Bioer Technology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simpli/extraction+kit+rna/pmc12537064-35-3-9
Average 86 stars, based on 1 article reviews
simply p virus rna extraction kit - by Bioz Stars, 2026-11
86/100 stars

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Isolation:

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells
Article Snippet: .. Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary DNA (cDNA) was synthesized from the extracted total RNA using the HiSenScript RH (-) cDNA synthesis kit (iNtRON Biotechnology, Seongnam, Korea).

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells.
Article Snippet: .. Assessment of ABCB1 & ABCG2 gene expression by reverse transcriptase -quantitative PCR (RT-qPCR) Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary 1 3 Page 3 of 15 175 Medical Oncology (2026) 43:175 and β-actin (1:1000, Abcam 8224).

RNA Extraction:

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells
Article Snippet: .. Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary DNA (cDNA) was synthesized from the extracted total RNA using the HiSenScript RH (-) cDNA synthesis kit (iNtRON Biotechnology, Seongnam, Korea).

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells.
Article Snippet: .. Assessment of ABCB1 & ABCG2 gene expression by reverse transcriptase -quantitative PCR (RT-qPCR) Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary 1 3 Page 3 of 15 175 Medical Oncology (2026) 43:175 and β-actin (1:1000, Abcam 8224).

Article Title: African swine fever virus pC147L inhibits retinoic acid-inducible gene I-like receptors pathway via targeting MAVS-TRAF6 complex
Article Snippet: .. Total RNA was extracted from PK-15 and PAM cells using a Simple P Total RNA Extraction Kit (Bioer Technology, Hangzhou, ZJ, China) and subsequently reverse-transcribed into cDNA using a HiScript II Q RT Kit (Vazyme Biotech). .. Each amplified fragment was then cloned into the pCAGGS-HA vector using a homologous recombination kit (Vazyme Biotech, Nanjing, JS, China) according to the manufacturer’s instructions.

Article Title: African swine fever virus pC147L inhibits retinoic acid-inducible gene I-like receptors pathway via targeting MAVS-TRAF6 complex.
Article Snippet: .. Total RNA was extracted from PK-15 and PAM cells using a Simple P Total RNA Extraction Kit (Bioer Technology, Hangzhou, ZJ, CN) and subsequently reverse-transcribed into cDNA using a HiScript II Q RT Kit (Vazyme Biotech, Nanjing, JS, CN). .. Each amplified fragment was then cloned into the pCAGGS-HA vector using a homologous recombination kit (Vazyme Biotech, Nanjing, JS, CN) according to the manufacturer’s instructions.

Gene Expression:

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells.
Article Snippet: .. Assessment of ABCB1 & ABCG2 gene expression by reverse transcriptase -quantitative PCR (RT-qPCR) Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary 1 3 Page 3 of 15 175 Medical Oncology (2026) 43:175 and β-actin (1:1000, Abcam 8224).

Reverse Transcription:

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells.
Article Snippet: .. Assessment of ABCB1 & ABCG2 gene expression by reverse transcriptase -quantitative PCR (RT-qPCR) Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary 1 3 Page 3 of 15 175 Medical Oncology (2026) 43:175 and β-actin (1:1000, Abcam 8224).

Article Title: African swine fever virus pC147L inhibits retinoic acid-inducible gene I-like receptors pathway via targeting MAVS-TRAF6 complex
Article Snippet: .. Total RNA was extracted from PK-15 and PAM cells using a Simple P Total RNA Extraction Kit (Bioer Technology, Hangzhou, ZJ, China) and subsequently reverse-transcribed into cDNA using a HiScript II Q RT Kit (Vazyme Biotech). .. Each amplified fragment was then cloned into the pCAGGS-HA vector using a homologous recombination kit (Vazyme Biotech, Nanjing, JS, China) according to the manufacturer’s instructions.

Article Title: African swine fever virus pC147L inhibits retinoic acid-inducible gene I-like receptors pathway via targeting MAVS-TRAF6 complex.
Article Snippet: .. Total RNA was extracted from PK-15 and PAM cells using a Simple P Total RNA Extraction Kit (Bioer Technology, Hangzhou, ZJ, CN) and subsequently reverse-transcribed into cDNA using a HiScript II Q RT Kit (Vazyme Biotech, Nanjing, JS, CN). .. Each amplified fragment was then cloned into the pCAGGS-HA vector using a homologous recombination kit (Vazyme Biotech, Nanjing, JS, CN) according to the manufacturer’s instructions.

Real-time Polymerase Chain Reaction:

Article Title: Enhanced antitumor activity of atorvastatin and luteolin, with or without doxorubicin, in a solid Ehrlich carcinoma mouse model: modulation of ABC transporters, telomerase, and cancer stem cells.
Article Snippet: .. Assessment of ABCB1 & ABCG2 gene expression by reverse transcriptase -quantitative PCR (RT-qPCR) Total RNA was isolated from the tumor tissue using a total RNA extraction kit (Bioer Technology, Binjiang, China) following the manufacturer’s guidelines. .. Complementary 1 3 Page 3 of 15 175 Medical Oncology (2026) 43:175 and β-actin (1:1000, Abcam 8224).

Virus:

Article Title: Unraveling VP2 mutations in Infectious Bursal Disease Virus (IBDV) associated with potential vaccine escape in poultry flocks in Shandong, China.
Article Snippet: .. Total viral RNA was extracted using the Simply P Virus DNA/RNA Co-Extraction Kit (Cat. No. BSC67, Bioer Technology Co., Hangzhou, China) according to the manufacturer’s protocol. ..

Polymerase Chain Reaction:

Article Title: Segmented filamentous bacteria are associated with disease activity in children with inflammatory bowel disease
Article Snippet: Thermal cycling consisted of 30 cycles of at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 45 s, followed by a final extension at 72 °C for 5 min. PCR amplicons were visualized using TAE agarose gel electrophoresis (1.0%) and a gel imager (Bio-Rad, California, USA). .. Flagellin PCR products were purified with a Biospin Gel Extraction Kit (Bioer Technology Co., Ltd., Hangzhou, China) and cloned into pMD18-T vectors (Takara, Dalian, China). .. Ten clones from each library were randomly selected for sequencing using vector- specific M13F primers (Sangon Biotech, Shanghai, China).

Purification:

Article Title: Segmented filamentous bacteria are associated with disease activity in children with inflammatory bowel disease
Article Snippet: Thermal cycling consisted of 30 cycles of at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 45 s, followed by a final extension at 72 °C for 5 min. PCR amplicons were visualized using TAE agarose gel electrophoresis (1.0%) and a gel imager (Bio-Rad, California, USA). .. Flagellin PCR products were purified with a Biospin Gel Extraction Kit (Bioer Technology Co., Ltd., Hangzhou, China) and cloned into pMD18-T vectors (Takara, Dalian, China). .. Ten clones from each library were randomly selected for sequencing using vector- specific M13F primers (Sangon Biotech, Shanghai, China).

Gel Extraction:

Article Title: Segmented filamentous bacteria are associated with disease activity in children with inflammatory bowel disease
Article Snippet: Thermal cycling consisted of 30 cycles of at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 45 s, followed by a final extension at 72 °C for 5 min. PCR amplicons were visualized using TAE agarose gel electrophoresis (1.0%) and a gel imager (Bio-Rad, California, USA). .. Flagellin PCR products were purified with a Biospin Gel Extraction Kit (Bioer Technology Co., Ltd., Hangzhou, China) and cloned into pMD18-T vectors (Takara, Dalian, China). .. Ten clones from each library were randomly selected for sequencing using vector- specific M13F primers (Sangon Biotech, Shanghai, China).

Article Title: An African Royal Flush: five crowned nudibranch species (Nudibranchia: Polyceridae) recovered from temperate southern Africa using integrative taxonomy
Article Snippet: .. Bands of gene amplicons were excised and cleaned following the manufacturer’s guidelines using Biospin Gel Extraction Kits (Bioflux, Bioer Technology Co., Ltd.). .. Bands of gene amplicons were excised and cleaned following the manufacturer’s guidelines using Biospin Gel Extraction Kits (Bioflux, Bioer Technology Co., Ltd.).

Clone Assay:

Article Title: Segmented filamentous bacteria are associated with disease activity in children with inflammatory bowel disease
Article Snippet: Thermal cycling consisted of 30 cycles of at 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 45 s, followed by a final extension at 72 °C for 5 min. PCR amplicons were visualized using TAE agarose gel electrophoresis (1.0%) and a gel imager (Bio-Rad, California, USA). .. Flagellin PCR products were purified with a Biospin Gel Extraction Kit (Bioer Technology Co., Ltd., Hangzhou, China) and cloned into pMD18-T vectors (Takara, Dalian, China). .. Ten clones from each library were randomly selected for sequencing using vector- specific M13F primers (Sangon Biotech, Shanghai, China).



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