nonlinear regression curve fitting package (sigmoidal dose–response)  (GraphPad Software Inc)

S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig. S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested." title="... assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: IC 50 and IC 90 values (μM) of anti-tumor drugs for monolayer and spheroid-cultured cells in a 96-well format (Fig. S2 , n = 3). Each cell culture was performed at 37 °C in an atmosphere containing 5% CO 2 , and treated with various concentrations of drugs for 48 h. Cell viability was assessed using the MTS assay reagent (Promega). Data were analyzed using a nonlinear regression curve-fitting package in Prism 4 (GraphPad Software Inc.) (Fig. S2 ). The drugs tested included: docetaxel, a mitotic inhibitor; irinotecan, a topoisomerase I inhibitor; 5-fluorouracil (5-FU), a thymidylate synthase inhibitor; sunitinib, a multi-receptor tyrosine kinase (RTK) inhibitor; tirapazamine, a hypoxia-activated prodrug; and FX11, a lactate dehydrogenase inhibitor. Radial organization of each cell on the spheroidal culture dish (IWAKI) conferred resistance to the most of drugs tested.

Techniques Used: Cell Culture, MTS Assay, Software