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respiration buffer  (Cytiva Europe)


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    Cytiva Europe respiration buffer
    Respiration Buffer, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 18577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+statistical+software+(version+12%2E5/HEPES/pmc07248293-50-10-1
    Average 98 stars, based on 18577 article reviews
    respiration buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Lysis:

    Article Title: Essential role of the CD docking motif of MPK4 in plant immunity, growth, and development.
    Article Snippet: The N-terminal GST tag of MPK4 and mutants was removed by overnight incubation with PreScission Protease (GE Healthcare) at 4°C. .. After GST cleavage, proteins were eluted with lysis buffer and further purified on a HiLoad16/60 Superdex 200 prep-grade gel filtration column (GE Healthcare) using a buffer containing 20 mM HEPES (pH 7.5), 150 mM NaCl, 3 mM DTT. ..

    Purification:

    Article Title: Essential role of the CD docking motif of MPK4 in plant immunity, growth, and development.
    Article Snippet: The N-terminal GST tag of MPK4 and mutants was removed by overnight incubation with PreScission Protease (GE Healthcare) at 4°C. .. After GST cleavage, proteins were eluted with lysis buffer and further purified on a HiLoad16/60 Superdex 200 prep-grade gel filtration column (GE Healthcare) using a buffer containing 20 mM HEPES (pH 7.5), 150 mM NaCl, 3 mM DTT. ..

    Article Title: A Cryptic Basic Groove formed by Ubiquitin and Histone H3 Mediates the Selective Recognition of H2AK119Ub Nucleosomes by Synovial Sarcoma X Breakpoint 1 Protein
    Article Snippet: .. The elution was diluted with IEX buffer A (25mM HEPES, pH 7.5) to a final 50 mM NaCl, then purified by Mono-Q column-based ion-exchange chromatography (GE Healthcare) (IEX buffer A: 25mM HEPES, pH 7.5; IEX buffer B: 25mM HEPES, 1M NaCl, pH 7.5). .. The aimed fractions analysed by 15% SDS-PAGE were mixed and concentrated and further purified by size-exclusion chromatography (GE Healthcare) equipped with Superdex 200 Increase 10/300 GL column and pre-equilibrated by SEC buffer (20mM HEPES, pH 7.5, 150mM NaCl, 1mM DTT, 5% glycerol).

    Article Title: The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase
    Article Snippet: For the purification of TNAP proteins, the supernatants of the cell cultures were collected by centrifugation and then subjected to the affinity chromatography with Nickel-chelating resin (Smart-Lifesciences, China). .. After eluted with a buffer comprised with 20 mM HEPES (pH 7.5), 150 mM NaCl, 10% glycerol, and 300 mM imidazole, the eluates were concentrated and the proteins of interest were further purified with size-exclusion chromatography using Superdex 200 Increase 10/300 GL (GE Healthcare, USA) column pre-equalized in a buffer comprised with 20 mM HEPES (pH 7.5), 150 mM NaCl. ..

    Filtration:

    Article Title: Essential role of the CD docking motif of MPK4 in plant immunity, growth, and development.
    Article Snippet: The N-terminal GST tag of MPK4 and mutants was removed by overnight incubation with PreScission Protease (GE Healthcare) at 4°C. .. After GST cleavage, proteins were eluted with lysis buffer and further purified on a HiLoad16/60 Superdex 200 prep-grade gel filtration column (GE Healthcare) using a buffer containing 20 mM HEPES (pH 7.5), 150 mM NaCl, 3 mM DTT. ..

    Isolation:

    Article Title: Neutrophil-derived reactive agents induce a transient SpeB negative phenotype in Streptococcus pyogenes
    Article Snippet: .. After isolation, neutrophils were suspended in RPMI1640 medium (HyClone) supplemented with 10 mM l -glutamine, 25 mmol/l HEPES (all HyClone), and 5% (v/v) FCS. ..

    Ion Exchange Chromatography:

    Article Title: A Cryptic Basic Groove formed by Ubiquitin and Histone H3 Mediates the Selective Recognition of H2AK119Ub Nucleosomes by Synovial Sarcoma X Breakpoint 1 Protein
    Article Snippet: .. The elution was diluted with IEX buffer A (25mM HEPES, pH 7.5) to a final 50 mM NaCl, then purified by Mono-Q column-based ion-exchange chromatography (GE Healthcare) (IEX buffer A: 25mM HEPES, pH 7.5; IEX buffer B: 25mM HEPES, 1M NaCl, pH 7.5). .. The aimed fractions analysed by 15% SDS-PAGE were mixed and concentrated and further purified by size-exclusion chromatography (GE Healthcare) equipped with Superdex 200 Increase 10/300 GL column and pre-equilibrated by SEC buffer (20mM HEPES, pH 7.5, 150mM NaCl, 1mM DTT, 5% glycerol).

    other:

    Article Title: Quantification of Proliferative and Dead Cells in Enteroids
    Article Snippet: Name Company Catalog Number 15 ml centrifuge tube Corning 430791 22 G gavage needle VWR 20068-608 24-well plate Nunc 142475 40 mm sterile cell strainer BD 352340 50 ml centrifuge tube Corning 430829 70 mm sterile cell strainer BD 352350 Advanced DMEM/F-12 GIBCO 12634010 Attune NxT Acoustic Focusing Cytometer Invitrogen A24863 B-27 Supplement GIBCO 17504044 Buffer 1 2 mM EDTA in DPBS Buffer 2 54.9 mM D-sorbitol, 43.4 mM sucrose in DPBS C57/B6 mice Nanjing Biomedical Research Institute of Nanjing University Cell-dissociation enzymes (TrypLE) Life technologies 12605-010 Centrifuge Eppendorf 5424R Centrifuge Eppendorf 5810R Click-iT Plus EdU Alexa Fluor 594 Imaging Kit Life technologies C10639 CO2 incubator Panasonic MCO-18AC DPBS D-sorbitol GIBCO BBI 14190144 SB0491 EDTA BBI EB0185 ENR media Minigut media, 50 ng/ml EGF, 100 ng/ml Noggin, 500 ng/ml R-spondin Fetal Bovine Serum (FBS) Gibco 10270-106 Fine Iris Scissors Fluorescence microscope Tansoole Olympus 2037454 FV1000 GlutaMAX Supplement GIBCO 35050-061 Goat Serum Life technologies 16210-064 HDMEM Hyclone SH30243.01B HEPES Sigma H4034 Matrigel Corning 356231 Minigut media Advanced DMEM/F12, 2 mM Glutamax, Penn/Strep (100 units/ ml), 10 mM Hepes, N2 supplement (1:100), B27 supplement (1:50) N2 supplement R&D AR009 Nonionic surfactant (Triton X) BBI TB0198-500ML Operating Scissor (12.5 cm) Tansoole 2025785 Paraformaldehyde (PFA) sigma 158127-500g Penn/Strep Invitrogen 15140-148 Phase contrast microscope Nikon TS1000 Propidium iodide Recombinant EGF Sigma PeproTech P4170-25MG 315-09 Recombinant Mouse Noggin PeproTech 250-38 Recombinant Mouse R-Spondin 1 R&D 3474-RS-050 Recombinant Murine IL-22 PeproTech 210-22-10 Sucrose BBI SB0498 Tissue Forceps Tansoole 2026704 Y-27632 2HC1 Selleck S1049 Open in a separate window MATERIALS REQUIRED

    Size-exclusion Chromatography:

    Article Title: The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase
    Article Snippet: For the purification of TNAP proteins, the supernatants of the cell cultures were collected by centrifugation and then subjected to the affinity chromatography with Nickel-chelating resin (Smart-Lifesciences, China). .. After eluted with a buffer comprised with 20 mM HEPES (pH 7.5), 150 mM NaCl, 10% glycerol, and 300 mM imidazole, the eluates were concentrated and the proteins of interest were further purified with size-exclusion chromatography using Superdex 200 Increase 10/300 GL (GE Healthcare, USA) column pre-equalized in a buffer comprised with 20 mM HEPES (pH 7.5), 150 mM NaCl. ..



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