connexin43 (Thermo Fisher)

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Thermo Fisher connexin43
Connexin43, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigma+plot+2001+software+package/Triton+X-100/pmc02735611-74-15-36
Average 99 stars, based on 1 article reviews

connexin43 - by Bioz Stars, 2026-10

99/100 stars

Images

Blocking Assay:

Article Title: Lateral sclerosis in amyotrophic lateral sclerosis is an upper motor neuron distal axonopathy ("dying back"): a clinical-neuropathological study.
Article Snippet: Astrocytes were labeled with anti-glial fibrillary acidic protein (GFAP) antibodies against human (rabbit polyclonal, 1:100; Cat. No. Z0334, lot 00076541, DAKO). .. The primary antibodies were diluted in blocking solution (10% normal goat serum, 0.05% Triton X-100 in phosphate-buffered saline) overnight at 4 °C followed by incubation with goat anti-rabbit Alexa Fluor 488 (1:100; Invitrogen, Carlsbad, CA). .. For CD68 immunostaining, biotin-conjugated goat anti-mouse was used as a secondary antibody; the signal was amplified using a tyramide signal amplification kit (1:250; Invitrogen) following the manufacturer’s instructions [62].

Article Title: Neonatal muscle-derived extracellular vesicles containing miR-542-3p rejuvenate aged skeletal muscle via a functional microneedle patch
Article Snippet: .. After washing frozen muscle sections or C2C12 cell coverslips with PBS, the samples were fixed 4% paraformaldehyde (PFA, Beyotime Biotechnology, China) for 10 min. After three PBS washes, the samples were permeabilized with 0.1% Triton X-100 in PBS for 15 min, followed by blocking with 3% bovine serum albumin (BSA, Thermo Fisher, USA) for 30 min. ..

Article Title: Type I IFN coordinates resistance and tolerance against Zika virus disease by inducing NK cell recruitment and limiting neutrophil-driven neuropathology in the brain
Article Snippet: .. Briefly, free-floating sections were washed with 0.3% Triton X-100 in PBS (3 × 10 min) and then incubated for 30 min in a blocking solution containing 5% normal goat serum (Thermo Fisher Scientific) and 0.3% Triton X-100 in PBS. .. For microglia and astrocyte staining, anti-Iba-1 (#019-19741; 1:100, Wako, Richmond, VA) and anti-GFAP (#z0334; 1:600, Dako Agilent) primary antibodies were diluted in blocking solution and incubated for 16h at 8°C.

Saline:

Article Title: Citron kinase activity controls alternative splicing events that drive prostate cancer growth
Article Snippet: Before incubation with recombinant CIT kinase domain or cell lysates, the arrays were blocked using 1% bovine serum albumin (BSA; Sigma-Aldrich) to avoid nonspecific binding. .. Following incubation, the arrays were washed with PBSTx buffer [1% Triton X-100 in 1× phosphate-buffered saline (PBS)] and incubated with first Phos-Tag Biotin solution (Nard Institute, 301-93531) for 2.5 hours at room temperature and then with HRP-streptavidin conjugate for 1.5 hours at room temperature in a humid chamber before detection using ECL system (Thermo Fisher Scientific, 34096). ..

Incubation:

Article Title: Placental TRPV2 supports trophoblast function and normal fetal development.
Article Snippet: .. Before use, the slides were dried (2min at 37 °C) and blocked with 5% normal donkey serum and 0.3% Triton X-100 in TBS for 1 h. Lectin (Isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor 488 Conjugate, Invitrogen) staining was performed at a 1:200 dilution and incubated for 1 hour at 37 °C. .. For MCT1/MCT4 staining, primary antibodies were incubated overnight at 4 °C: MCT1 (No. AB1286I, antimonocarboxylate transporter 1 Polyclonal antibody, Millipore) at a 1:500 dilution (1 μg/ml) and MCT4 (No. AB3314P, formerly AB3546P, rabbit anti-monocarboxylate transporter 4 Polyclonal antibody, Millipore) at a 1:100 dilution (10 μg/ml) in TBS.

Western Blot:

Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

Transcranial Magnetic Stimulation:

Staining:

Article Title: Arachidonic Acid Disrupts PKM2 ‐Linked Glycolysis‐Related Metabolism and Exacerbates PAN ‐Induced Podocyte Injury
Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with PBS containing 0.3% Triton X‐100, blocked with 5% BSA for 30 min, and stained with 500 nM Alexa Fluor 488 phalloidin (Invitrogen) for 30 min and 1 × Hoechst for 15 min. Phalloidin‐labelled F‐actin (green) and Hoechst stain cellular DNA (blue). ..

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