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protein a g agarose  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology protein a g agarose
    Protein A G Agarose, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 13922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+2001+software+package/Protein+A%2FG+PLUS-Agarose/pm22002310-234-28-41
    Average 96 stars, based on 13922 article reviews
    protein a g agarose - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: A Toxoplasma gondii-derived ROP38 protein engages Ser15/Thr18-linked p53 activation and DNA damage-associated apoptotic signaling in human cancer cells.
    Article Snippet: Recombinant human p53 protein was purchased from Active Motif (Carlsbad, CA, USA; 81091). .. Recombinant protein-based direct interaction assay To examine the direct association between recombinant ROP38 Full and human p53 in the absence of cellular lysates, a recombinant protein-based co-immunoprecipitation assay was performed using Protein A/G PLUSAgarose beads (Santa Cruz Biotechnology; sc-2003). ..

    Co-Immunoprecipitation Assay:

    Article Title: A Toxoplasma gondii-derived ROP38 protein engages Ser15/Thr18-linked p53 activation and DNA damage-associated apoptotic signaling in human cancer cells.
    Article Snippet: Recombinant human p53 protein was purchased from Active Motif (Carlsbad, CA, USA; 81091). .. Recombinant protein-based direct interaction assay To examine the direct association between recombinant ROP38 Full and human p53 in the absence of cellular lysates, a recombinant protein-based co-immunoprecipitation assay was performed using Protein A/G PLUSAgarose beads (Santa Cruz Biotechnology; sc-2003). ..

    Incubation:

    Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics.
    Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with protein A/G plus-agarose (5 μl/μg antibody, Santa Cruz). ..

    Article Title: AKT Phosphorylation of FOXK2 at Ser398 Impacts Breast Cancer Cell Growth and Viability
    Article Snippet: .. About 3–4 mg of the whole- cell extract were then incubated overnight with A/G PLUS- Agarose (sc2003; Santa Cruz Biotechnology, Dallas, TX, USA) coupled with the primary antibodies at 4°C (FOXK2, 12 008; Phospho- AKT, 9271, Cell Signaling, Boston, MA, USA). .. Since caspase- 1 and FOXK2 are not reported to interact, resins conjugated to anticaspase- 1 antibody (sc- 515, Santa Cruz Biotechnology) were used as a negative control.

    Article Title: HECTD1 Directs HIF‐1α to Facilitate Intestinal Inflammation and Carcinogenesis via Promoting Th17 Differentiation and Glycolysis
    Article Snippet: Stimulated CD4 + T cells were rinsed with ice‐cold PBS and lysed in RIPA buffer containing protease inhibitor (Sigma‐Aldrich) and 1 mM PMSF for 30 min at 4°C, followed by centrifugation to obtain supernatant. .. For immunoprecipitation (IP), the supernatants were immunoprecipitated overnight at 4°C with the appropriate antibody and then incubated with washed protein A/G‐agarose (Santa Cruz Biotechnology) for another 2 h at 4°C. ..

    Cell-Signaling:

    Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics.
    Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with protein A/G plus-agarose (5 μl/μg antibody, Santa Cruz). ..

    Article Title: AKT Phosphorylation of FOXK2 at Ser398 Impacts Breast Cancer Cell Growth and Viability
    Article Snippet: .. About 3–4 mg of the whole- cell extract were then incubated overnight with A/G PLUS- Agarose (sc2003; Santa Cruz Biotechnology, Dallas, TX, USA) coupled with the primary antibodies at 4°C (FOXK2, 12 008; Phospho- AKT, 9271, Cell Signaling, Boston, MA, USA). .. Since caspase- 1 and FOXK2 are not reported to interact, resins conjugated to anticaspase- 1 antibody (sc- 515, Santa Cruz Biotechnology) were used as a negative control.

    Control:

    Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics.
    Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with protein A/G plus-agarose (5 μl/μg antibody, Santa Cruz). ..

    Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer
    Article Snippet: Briefly cells were crosslinked with 0.75% formaldehyde (Sigma-Aldrich, USA), quenched with glycine (BioRad, USA), lysed and chromatin was sheared by sonication (VCX750, Sonics, USA) to obtain fragments of 200 to 500 bp size. .. 5% of total sheared chromatin was reserved as input and immunoprecipitation performed overnight at 4 °C using antibodies listed in Supplementary Table or normal-rabbit IgG (Merck Millipore, USA) (non-immune mock control), with Protein A/G plus agarose beads (Santa Cruz Biotechnology, USA). .. After washing, bound chromatin was eluted and reverse crosslinked and DNA purified using Wizard SV Gel and PCR Clean-Up System (Promega, USA).

    Pull Down Assay:

    Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics.
    Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with protein A/G plus-agarose (5 μl/μg antibody, Santa Cruz). ..

    Clarification Assay:

    Article Title: The SGLT2 Inhibitor Empagliflozin Directly Binds Cardiac JNK3, Uncouples c-Jun/c-Fos Signaling, and Confers Cardioprotection in Diabetic Cardiomyopathy.
    Article Snippet: Cells were lysed at 4°C in NP-40 buffer (Supplementary Table 1) supplemented with protease and phosphatase inhibitors (Roche, Indianapolis, IN, USA; 11836170001). .. After clarification at 14,000 ×g for 10 minutes at 4°C and protein quantification, equal lysate amounts were precleared with protein A/G agarose (Santa Cruz, Santa Cruz, CA, USA; SC2003) for 30 minutes at 4°C. ..

    Immunoprecipitation:

    Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer
    Article Snippet: Briefly cells were crosslinked with 0.75% formaldehyde (Sigma-Aldrich, USA), quenched with glycine (BioRad, USA), lysed and chromatin was sheared by sonication (VCX750, Sonics, USA) to obtain fragments of 200 to 500 bp size. .. 5% of total sheared chromatin was reserved as input and immunoprecipitation performed overnight at 4 °C using antibodies listed in Supplementary Table or normal-rabbit IgG (Merck Millipore, USA) (non-immune mock control), with Protein A/G plus agarose beads (Santa Cruz Biotechnology, USA). .. After washing, bound chromatin was eluted and reverse crosslinked and DNA purified using Wizard SV Gel and PCR Clean-Up System (Promega, USA).

    Article Title: HECTD1 Directs HIF‐1α to Facilitate Intestinal Inflammation and Carcinogenesis via Promoting Th17 Differentiation and Glycolysis
    Article Snippet: Stimulated CD4 + T cells were rinsed with ice‐cold PBS and lysed in RIPA buffer containing protease inhibitor (Sigma‐Aldrich) and 1 mM PMSF for 30 min at 4°C, followed by centrifugation to obtain supernatant. .. For immunoprecipitation (IP), the supernatants were immunoprecipitated overnight at 4°C with the appropriate antibody and then incubated with washed protein A/G‐agarose (Santa Cruz Biotechnology) for another 2 h at 4°C. ..



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