protein a g agarose (Santa Cruz Biotechnology)
96
Structured Review
Santa Cruz Biotechnology
protein a g agarose
Protein A G Agarose, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 13922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigma+plot+2001+software+package/Protein+A%2FG+PLUS-Agarose/pm22002310-234-28-41
Average 96 stars, based on 13922 article reviews
Protein A G Agarose, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 13922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigma+plot+2001+software+package/Protein+A%2FG+PLUS-Agarose/pm22002310-234-28-41
Average 96 stars, based on 13922 article reviews
protein a g agarose - by Bioz Stars,
2026-10
96/100 stars
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Recombinant:Article Title: A Toxoplasma gondii-derived ROP38 protein engages Ser15/Thr18-linked p53 activation and DNA damage-associated apoptotic signaling in human cancer cells. Article Snippet: Recombinant human p53 protein was purchased from Active Motif (Carlsbad, CA, USA; 81091). .. Recombinant protein-based direct interaction assay To examine the direct association between recombinant ROP38 Full and human p53 in the absence of cellular lysates, a recombinant protein-based co-immunoprecipitation assay was performed using Co-Immunoprecipitation Assay:Article Title: A Toxoplasma gondii-derived ROP38 protein engages Ser15/Thr18-linked p53 activation and DNA damage-associated apoptotic signaling in human cancer cells. Article Snippet: Recombinant human p53 protein was purchased from Active Motif (Carlsbad, CA, USA; 81091). .. Recombinant protein-based direct interaction assay To examine the direct association between recombinant ROP38 Full and human p53 in the absence of cellular lysates, a recombinant protein-based co-immunoprecipitation assay was performed using Incubation:Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics. Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with Article Title: AKT Phosphorylation of FOXK2 at Ser398 Impacts Breast Cancer Cell Growth and Viability Article Snippet: .. About 3–4 mg of the whole- cell extract were then incubated overnight with Article Title: HECTD1 Directs HIF‐1α to Facilitate Intestinal Inflammation and Carcinogenesis via Promoting Th17 Differentiation and Glycolysis Article Snippet: Stimulated CD4 + T cells were rinsed with ice‐cold PBS and lysed in RIPA buffer containing protease inhibitor (Sigma‐Aldrich) and 1 mM PMSF for 30 min at 4°C, followed by centrifugation to obtain supernatant. .. For immunoprecipitation (IP), the supernatants were immunoprecipitated overnight at 4°C with the appropriate antibody and then incubated with washed Cell-Signaling:Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics. Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with Article Title: AKT Phosphorylation of FOXK2 at Ser398 Impacts Breast Cancer Cell Growth and Viability Article Snippet: .. About 3–4 mg of the whole- cell extract were then incubated overnight with Control:Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics. Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer Article Snippet: Briefly cells were crosslinked with 0.75% formaldehyde (Sigma-Aldrich, USA), quenched with glycine (BioRad, USA), lysed and chromatin was sheared by sonication (VCX750, Sonics, USA) to obtain fragments of 200 to 500 bp size. .. 5% of total sheared chromatin was reserved as input and immunoprecipitation performed overnight at 4 °C using antibodies listed in Supplementary Table or normal-rabbit IgG (Merck Millipore, USA) (non-immune mock control), with Pull Down Assay:Article Title: G6PD suppresses thrombopoiesis via interfering with TMOD3-mediated actin dynamics. Article Snippet: .. The lysates (supernatants) were 16 EMBO reports © The Author(s) incubated with anti-G6PD antibody (6 μg/mL, Abcam) and normal rabbit IgG (6 μg/mL, Cell Signaling Technology) as control overnight, and then pull down with Clarification Assay:Article Title: The SGLT2 Inhibitor Empagliflozin Directly Binds Cardiac JNK3, Uncouples c-Jun/c-Fos Signaling, and Confers Cardioprotection in Diabetic Cardiomyopathy. Article Snippet: Cells were lysed at 4°C in NP-40 buffer (Supplementary Table 1) supplemented with protease and phosphatase inhibitors (Roche, Indianapolis, IN, USA; 11836170001). .. After clarification at 14,000 ×g for 10 minutes at 4°C and protein quantification, equal lysate amounts were precleared with Immunoprecipitation:Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer Article Snippet: Briefly cells were crosslinked with 0.75% formaldehyde (Sigma-Aldrich, USA), quenched with glycine (BioRad, USA), lysed and chromatin was sheared by sonication (VCX750, Sonics, USA) to obtain fragments of 200 to 500 bp size. .. 5% of total sheared chromatin was reserved as input and immunoprecipitation performed overnight at 4 °C using antibodies listed in Supplementary Table or normal-rabbit IgG (Merck Millipore, USA) (non-immune mock control), with Article Title: HECTD1 Directs HIF‐1α to Facilitate Intestinal Inflammation and Carcinogenesis via Promoting Th17 Differentiation and Glycolysis Article Snippet: Stimulated CD4 + T cells were rinsed with ice‐cold PBS and lysed in RIPA buffer containing protease inhibitor (Sigma‐Aldrich) and 1 mM PMSF for 30 min at 4°C, followed by centrifugation to obtain supernatant. .. For immunoprecipitation (IP), the supernatants were immunoprecipitated overnight at 4°C with the appropriate antibody and then incubated with washed |