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peroxidase  (Jackson Immuno)


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    Structured Review

    Jackson Immuno peroxidase
    Peroxidase, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+12%2E3+software/Goat+Peroxidase-Anti-Peroxidase/pmc02311449-125-12-22
    Average 96 stars, based on 682 article reviews
    peroxidase - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Tissue:

    Article Title: Impact of NFAT5 conditional knockout on murine salivary glands
    Article Snippet: For immunolabelling, deparaffined and rehydrated tissue sections were subjected to heat antigen retrieval in a 0.1 M citrate buffer pH6.0, incubation with 1% hydrogen peroxide diluted in 10mM phosphate buffer saline Article in Press Cell Communication and Signaling https://doi.org/10.1186/s12964-026-03237-9 pH7.4 (PBS) for 30 min at room temperature, and incubation for 15 min at room temperature (RT) with 5% normal donkey serum (NDS) (Jackson Immunoresearch, Cambridge, UK) diluted in PBS. .. Tissue sections were then incubated with primary antibodies overnight at 4°C (1/400 goat anti-rAQP5 antibodies diluted in 5% NDS – see below), rinsed with PBS, incubated for 15 min at RT with 5% NDS, incubated with secondary antibodies (1/35 donkey anti-goat diluted in 5% (Jackson Immunoresearch, Cambridge, UK)) for 15 min at RT, rinsed in PBS, incubated with 1/400 goat peroxidase-anti-peroxidase (Jackson Immunoresearch, Cambridge, UK) for 15 min at RT, rinsed in PBS, and finally incubated with 3,3’-diaminobenzidine (DAB; Agilent, Santa Clara, CA, USA). .. The slides were counterstained with hematoxylin prior to mounting with a coverslip using DPX mounting solution (Sigma-Aldrich - Merck, St Louis, MO, USA).

    Incubation:

    Article Title: Impact of NFAT5 conditional knockout on murine salivary glands
    Article Snippet: For immunolabelling, deparaffined and rehydrated tissue sections were subjected to heat antigen retrieval in a 0.1 M citrate buffer pH6.0, incubation with 1% hydrogen peroxide diluted in 10mM phosphate buffer saline Article in Press Cell Communication and Signaling https://doi.org/10.1186/s12964-026-03237-9 pH7.4 (PBS) for 30 min at room temperature, and incubation for 15 min at room temperature (RT) with 5% normal donkey serum (NDS) (Jackson Immunoresearch, Cambridge, UK) diluted in PBS. .. Tissue sections were then incubated with primary antibodies overnight at 4°C (1/400 goat anti-rAQP5 antibodies diluted in 5% NDS – see below), rinsed with PBS, incubated for 15 min at RT with 5% NDS, incubated with secondary antibodies (1/35 donkey anti-goat diluted in 5% (Jackson Immunoresearch, Cambridge, UK)) for 15 min at RT, rinsed in PBS, incubated with 1/400 goat peroxidase-anti-peroxidase (Jackson Immunoresearch, Cambridge, UK) for 15 min at RT, rinsed in PBS, and finally incubated with 3,3’-diaminobenzidine (DAB; Agilent, Santa Clara, CA, USA). .. The slides were counterstained with hematoxylin prior to mounting with a coverslip using DPX mounting solution (Sigma-Aldrich - Merck, St Louis, MO, USA).

    Article Title: Disrupted transcriptional networks regulated by CHD1L during neurodevelopment underlie the mirrored neuroanatomical and growth phenotypes of the 1q21.1 copy number variant
    Article Snippet: .. Membranes were washed and incubated in goat antirabbit and Goat antirat peroxidase secondary antibody (1:10 000, Jackson Immuno Research, U.K.). .. Membranes were washed and incubated in goat antirabbit and Goat antirat peroxidase secondary antibody (1:10 000, Jackson Immuno Research, U.K.).

    other:

    Article Title: Disrupted transcriptional networks regulated by CHD1L during neurodevelopment underlie the mirrored neuroanatomical and growth phenotypes of the 1q21.1 copy number variant
    Article Snippet: Resolved proteins were transferred to nitrocellulose membranes and blocked in 3% milk 1 × TBS for 1 h at room temperature prior to incubation with either anti-CHD1L (2170C3a) antibody (Santa Cruz, sc-81065, 1:200), anti-β tubulin (1:10 000, produced in house), and anti-TUJ1 (D71G9) (Cell Signaling Technology, 5568S, 1:1000).

    Article Title: Disrupted transcriptional networks regulated by CHD1L during neurodevelopment underlie the mirrored neuroanatomical and growth phenotypes of the 1q21.1 copy number variant
    Article Snippet: Resolved proteins were transferred to nitrocellulose membranes and blocked in 3% milk 1 × TBS for 1 h at room temperature prior to incubation with either anti-CHD1L (2170C3a) antibody (Santa Cruz, sc-81065, 1:200) or anti-β-Tubulin (1:10 000, produced in house).



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