research grade mintenyi (Miltenyi Biotec)

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Miltenyi Biotec research grade mintenyi
Research Grade Mintenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigma+plot+12%2E3+software/Human+CNTF%2C+research+grade/pm37967557-196-173-204
Average 93 stars, based on 14 article reviews

research grade mintenyi - by Bioz Stars, 2026-11

93/100 stars

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In Vitro:

Article Title: Altered mitochondria-associated ER membrane (MAM) function shifts mitochondrial metabolism in amyotrophic lateral sclerosis (ALS)
Article Snippet: The resulting pellet was resuspended in N2/B-27 media with the Day 0 differentiation factors listed above, and the cells were plated on poly-L-lysine (R&D Systems 3438-200-01) and laminin (4 μg/mL, MilliporeSigma L2020) coated surfaces. .. The cells were differentiated in vitro for 2, 7 or 14 days (unless otherwise indicated) in neuronal differentiation media [Neurobasal medium containing N2 supplement, B-27 supplement with vitamin A (ThermoFisher Scientific 17504044), pen/strep, L-glutamine, non-essential amino acids (NEAA, ThermoFisher Scientific 11140076), 1 μM antimitotic U-FdU (stock 10 mM uridine, MilliporeSigma U3750; stock 10 mM 5-fluoro-2'-deoxyuridine, MilliporeSigma F0503), 10 ng/mL GDNF, 10 ng/mL BDNF, 10 ng/mL ciliary neurotrophic factor (CNTF, Miltenyi Biotec 130-096-337), 25 μM L-glutamic acid (MilliporeSigma G5889), 25 μM ß-mercaptoethanol, 10 μM forskolin (Stemcell Technologies 72114), and 100 μM 3-isobutyl-1-methylxantine (IBMX, R&D Systems 2845/50)]. .. All cell lines used were routinely tested for mycoplasma following manufacturer instructions (Genlantis TM MycoScope TM PCR Detection Kit, Fisher Scientific MY01050).

Recombinant:

Article Title: Single-cell transcriptomics of human traumatic brain injury reveals activation of endogenous retroviruses in oligodendroglia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-STAT1, monoclonal Abcam Cat# ab109320; RRID:AB_10863383 Mouse anti-STAT1, monoclonal Abcam Cat# ab281999 Rabbit anti-OLIG2, polyclonal Sigma-Aldrich Cat# AB9610; RRID:AB_570666 Goat anti-OLIG2, polyclonal R and D Systems Cat# AF2418; RRID:AB_2157554 Chicken anti-Vimentin, polyclonal Millipore Cat# AB5733; RRID:AB_11212377 Mouse anti-S-100beta, monoclonal Sigma-Aldrich Cat# S2532; RRID:AB_477499 Rabbit anti-AQP4, polyclonal Sigma-Aldrich Cat# HPA014784; RRID:AB_1844967 Rabbit anti-GFAP, polyclonal DAKO; Sigma-Aldrich Cat# Z0334; RRID:AB_10013382 Chemicals, peptides, and recombinant proteins Laminin-521 Biolamina Cat# LN521 Laminin-111 Biolamina Cat# LN111-02 Penicillin-Streptomycin GIBCO (ThermoFisher) Cat# 15140122 StemPro Accutase GIBCO (ThermoFisher) Cat# A11105-01 StemMACS iPS-Brew XF, human Miltenyi biotec Cat# 130-104-368 DMEM/F12, w/Glutamax Invitrogen Cat# 31331-093 StemMACS SB431542 in solution Miltenyi Cat# 130-106-543 Human Noggin, research grade Miltenyi Cat# 130-103-456 Human SHH (C24II), premium grade Miltenyi Cat# 130-095-730 Human FGF-8b, premium grade Miltenyi Cat# 130-095-740 MACS NeuroMedium Miltenyi Cat# 130-093-570 StemMacs CHIR99021 in solution Miltenyi Cat# 130-106-539 N2 supplement Invitrogen Cat# 17502048 NB21 supplement Miltenyi Cat# 130-097-263 L-Glutamine GIBCO (ThermoFisher) Cat# 25030032 Rock-inhibitor, Y27632 Miltenyi Biotec Cat# 130-106-538 Non-Essential Amino Acids (NEAA) GIBCO (ThermoFisher) Cat# 11140 CNTF, Research grade Mintenyi Cat# 130-108-972 BDNF, Research grade Mintenyi Cat# 130-096-286 GDNF, premium grade Mintenyi Cat# 130-129-542 L-ASCORBIC ACID Sigma-Aldrich Cat# A4403-100MG Dibutyryl-cAMP Sigma-Aldrich Cat# D0627-1G NB-21 supplement without vitamin A Miltenyi biotec Cat# 130-093-566 PBS ThermoFisher Cat# AM9625 EGF Invitrogen Cat# PHG0311 FGF2, premium grade Miltenyi Cat# 130-093-564 PFA GIBCO (ThermoFisher) Cat# 28908 IFN-g, premium grade Miltenyi biotec Cat#130-096-482 Critical commercial assays Chromium Single Cell 30 Library 10X Genomics Cat# PN-1000268 Rneasy mini kit (miniRNeasy) QIAGEN Cat# 74104 TruSeq Stranded mRNA Illumina Cat# 20020594 Deposited data Raw and processed data of single-nuclei and bulk RNA-seq of the control postmortem samples and cell cultures This paper GSE209552 (Continued on next page) 14 Cell Reports 42, 113395, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Visualization, statistical analyses, and preprocessing pipelines, including QC filtering for the single nuclei RNAseq and bulk RNAseq This paper https://github.com/raquelgarza/TBI_Garza_2023; https://doi.org/10.5281/zenodo.8392734 Experimental models: Cell lines Human Embryonic stem cells (RC17) Roslin Cells RRID:CVCL_L206 Software and algorithms Seurat 10.1016/j.cell.2019.05.031 RRID:SCR_007322 STAR 10.1093/bioinformatics/bts635 RRID:SCR_004463 TEcount 10.1093/bioinformatics/btv422 RRID:SCR_023208 featureCounts 10.1093/bioinformatics/btt656 RRID:SCR_012919 deeptools 10.1093/nar/gku365 RRID:SCR_016366 CellRanger 10.1038/ncomms14049 RRID:SCR_017344 Integrative Genomics Viewer 10.1038/nbt.1754 RRID:SCR_011793 DESeq2 10.1186/s13059-014-0550-8 RRID:SCR_015687 bamtofastq NA RRID: SCR_023215 subset-bam NA RRID:SCR_023216 trusTEr 10.5281/zenodo.7589548 Other Paraffin hardware Tissue tek VIP; Sakura Finetek Tissue-Tek Cat# 62580-01 Buffered formalin Histo-Lab Products AB Cat# 02176 Blocking buffer/permeabilization buffer (Triton X-100) Cell Signaling Technology Cat# 39487 DAPI Sigma-Aldrich Cat# D9542

Magnetic Cell Separation:

Single Cell:

RNA Sequencing:

Control:

other:

Article Title: Massively parallel functional dissection of schizophrenia-associated noncoding genetic variants.
Article Snippet: Article Massively parallel functional dissection of schizophrenia-associated noncoding genetic variants

Article Title: GPR17 structure and agonism with small molecules and oxysterols
Article Snippet: For mouse OPC O4+ cells, the PhenoPlate-96 were pre-coated with poly-D-lysine, and cells seeded at a density of 20,000 cells/well in Differentiation Media plus CNTF (Miltenyi Biotec, 130-096-336, 10 ng/mL).

Cell Culture:

Article Title: An induced pluripotent stem cell-based chemical genetic approach for studying spinal muscular atrophy
Article Snippet: .. On day 4, cells were cultured in NBM basal media supplemented with doxycycline (2 μg/mL), BDNF (10 ng/mL; Miltenyi Biotec, Cat. No. 130-103-435), CNTF (10 ng/mL; Miltenyi Biotec, Cat. No. 130-123-659), and GDNF (10 ng/mL; Miltenyi Biotec, Cat. No. 130-108-986). .. On day 5, cells were exposed to NBM basal media supplemented with doxycycline (2 μg/mL) (Sigma Aldrich, Cat. No. D9891), BDNF (10 ng/mL), CNTF (10 ng/mL), GDNF (10 ng/mL), and U/FDU (10 μM).

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