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benzonase nuclease  (Cytiva Europe)


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    Structured Review

    Cytiva Europe benzonase nuclease
    Benzonase Nuclease, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 9372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+12%2E3+software/Percoll/pm31150623-557-72-66
    Average 97 stars, based on 9372 article reviews
    benzonase nuclease - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: Diet-mediated constitutive induction of novel IL-4 + ILC2 cells maintains intestinal homeostasis in mice
    Article Snippet: An aliquot of epithelial cells was washed with 10 ml of HBSS containing 5 mM EDTA, resuspended in 5 ml of 20% Percoll (GE Healthcare), and underlaid with 2.5 ml of 40% Percoll in a 15 ml Falcon tube to isolate colonic tuft cells.

    Article Title: Diet-mediated constitutive induction of novel IL-4 + ILC2 cells maintains intestinal homeostasis in mice
    Article Snippet: The digested tissues were washed with 10 ml of HBSS containing 5 mM EDTA, resuspended in 5 ml of 40% Percoll (GE Healthcare), and underlaid with 2.5 ml of 80% Percoll in a 15 ml Falcon tube.

    Gradient Centrifugation:

    Article Title: TRIM32 reduced the recruitment of innate immune cells and the killing capacity of Listeria monocytogenes by inhibiting secretion of chemokines
    Article Snippet: Livers were minced through a 100 μm cell strainer and digested for 30 min at 37 °C with rotation in HBSS (Ca 2+ , Mg 2+ ) buffer containing 1 mg/ml (335 U/mg) collagenase IV (Sangon Biotech, Shanghai, China) and 200 μg/ml (100 Kunitz U/ml) DNase I (Sangon Biotech, Shanghai, China). .. Liver leukocytes were separated by percoll gradient centrifugation (GE Healthcare, Freiburg, Germany). ..

    Isolation:

    Article Title: A diet rich in fermentable fiber promotes robust changes in the intestinal microbiota, mitigates intestinal permeability, and attenuates autoimmune uveitis
    Article Snippet: The cecum and colon were cut into 1 cm-long pieces and were digested in RPMI media with 5 mM EDTA (37 °C, 30 min × 2) and subsequently with 0.1 mg/ml collagenase II (Sigma-Aldrich) and 0.1 mg/ml DNase I (Roche, San Francisco, CA, USA) at 37 °C for 45 min twice (total 90 min). .. LPL were isolated after resuspending digested intestinal tissues in 30% Percoll (GE Healthcare, Pittsburgh, PA, USA) and then centrifuging the tissues layered on a 40%/60% Percoll gradient at 740 × g for 20 min. Two eyes from one animal were pooled. ..

    Purification:

    Article Title: Functional virus-specific memory T cells survey glioblastoma
    Article Snippet: .. Tumors were minced and digested in Collagenase type IV with DNAse for 30 min then dissociated via gentleMACS Dissociator, and lymphocytes purified on a 44/67% Percoll (GE Healthcare) gradient. .. Lymphocytes were stained for antihuman HLA-A2 (clone BB7.2, BioLegend, 343324), CCR7 (clone G043H7, BioLegend, 353208), CD45RO (clone UCHL1, BioLegend, 304232), CD8a (clone SK1, BD Biosciences, 561945), CD3e (clone SP34–2, BD Biosciences, 557917), CD4 (clone L200, BD Biosciences, 551980), CD69 (clone FN50, BioLegend, 310926), and CD103 (clone HML-1, Beckman Coulter, IM1856U).

    Article Title: Functional virus-specific memory T cells survey glioblastoma
    Article Snippet: .. In brief, GBM tumors were removed, digested in Collagenase IV (Sigma) with DNAse for 30 min, then dissociated via gentleMACS Dissociator (Miltenyi Biotec) and lymphocytes purified on a 44/67% Percoll (GE Healthcare) gradient. .. Isolated mouse cells were stained with antibodies to CD103 (clone 2E7, eBioscience, 17–1031-80), NK1.1 (clone PK136, BioLegend, 108728), CD8a (clone 53–6.7, BioLegend, 100743), IFNγ (clone XMG1.2, BD Biosciences, 54411), CD25 (clone PC61, BD Biosciences, 557192), CD44 (clone IM7, BioLegend, 103059), granzyme B (clone GB11, Invitrogen, GRB04), CD69 (clone H1.2F3, BD Biosciences, 562455), and Ki67 (clone SolA15, Invitrogen, 25–5698-82).



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