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anti nsf  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti nsf
    Anti Nsf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+12%2E3+software/NSF+Antibody/pm16102538-274-57-49
    Average 91 stars, based on 31 article reviews
    anti nsf - by Bioz Stars, 2026-11
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Extreme parsimony in ATP consumption by 20S complexes in the global disassembly of single SNARE complexes.
    Article Snippet: .. In all, 5 μg/mL secondary antibody (i.e., biotinylated goat anti-mouse or rabbit IgG antibody) and 5 μg/mL primary antibody (i.e., mouse monoclonal Anti-NSF (Santa Cruz Biotechnology) or rabbit polyclonal Anti-SNAP tag (New England Biolabs)) were added and incubated for 5 min. After removing the free antibodies by washing, 1 nM NSF for anti-N-domain or 20 pM NSF for anti-SNAP tag with 1 mM ATP and 1 mM EDTA was added and incubated for 5 min. ..

    other:

    Article Title: A comprehensive proteomics analysis of JC virus Agnoprotein-interacting proteins: Agnoprotein primarily targets the host proteins with coiled-coil motifs
    Article Snippet: Beads were then washed 3 times with lysis incubation buffer and protein complexes were resolved on a SDS-8% PAGE, transferred onto a nitrocellulose membrane with 0.2 μm pore size (LI-COR Odyssey, catalog no. P/N 926–31092) for 3 h at 250 mA.

    Article Title: A comprehensive proteomics analysis of JC virus Agnoprotein-interacting proteins: Agnoprotein primarily targets the host proteins with coiled-coil motifs
    Article Snippet: At 14 d posttransfection/infection, whole-cell extracts were prepared in TNN lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA and 1.0% NP-40] in the presence of protease inhibitors (Sigma, catalog no. P8340)] on a rocking platform at 4 °C for 30 min (2 × 10 6 cells/1000 μl lysis buffer).

    Lysis:

    Article Title: DEK::NUP214 acts as an XPO1-dependent transcriptional activator of essential leukemia genes
    Article Snippet: .. After protein quantification, 500 μg of protein was taken and added 25 μL of anti-XPO1 (Santa Cruz Biotechnology, sc-74457) or 30 μL of anti-HA (Cell Signaling, #2367) per condition and the volume was made up to 500 μL total using Lysis Buffer. ..

    Western Blot:

    Article Title: DEK::NUP214 acts as an XPO1-dependent transcriptional activator of essential leukemia genes
    Article Snippet: .. Western blotting was performed as described above on immunoprecipitated samples (DN-HA-293T and EV-293T for IP anti-XPO1 and anti-HA) and input using the following antibodies: anti-XPO1 (1:100 dilution, Santa Cruz Biotechnology, sc-74457), anti-HA (1:1000 dilution, Cell Signaling, #2367), or anti-GAPDH (1:5000 dilution, as a loading control). .. Cleavage under targets and release using nuclease Assay (CUT & Run) was performed according to the EpiCypher CUTANA CUT&Run protocol in DN-HA-293T and EV-293FT cells and FKH-1 and KASUMI-1 cell lines as previously described [4].

    Immunoprecipitation:

    Article Title: DEK::NUP214 acts as an XPO1-dependent transcriptional activator of essential leukemia genes
    Article Snippet: .. Western blotting was performed as described above on immunoprecipitated samples (DN-HA-293T and EV-293T for IP anti-XPO1 and anti-HA) and input using the following antibodies: anti-XPO1 (1:100 dilution, Santa Cruz Biotechnology, sc-74457), anti-HA (1:1000 dilution, Cell Signaling, #2367), or anti-GAPDH (1:5000 dilution, as a loading control). .. Cleavage under targets and release using nuclease Assay (CUT & Run) was performed according to the EpiCypher CUTANA CUT&Run protocol in DN-HA-293T and EV-293FT cells and FKH-1 and KASUMI-1 cell lines as previously described [4].

    Control:

    Article Title: DEK::NUP214 acts as an XPO1-dependent transcriptional activator of essential leukemia genes
    Article Snippet: .. Western blotting was performed as described above on immunoprecipitated samples (DN-HA-293T and EV-293T for IP anti-XPO1 and anti-HA) and input using the following antibodies: anti-XPO1 (1:100 dilution, Santa Cruz Biotechnology, sc-74457), anti-HA (1:1000 dilution, Cell Signaling, #2367), or anti-GAPDH (1:5000 dilution, as a loading control). .. Cleavage under targets and release using nuclease Assay (CUT & Run) was performed according to the EpiCypher CUTANA CUT&Run protocol in DN-HA-293T and EV-293FT cells and FKH-1 and KASUMI-1 cell lines as previously described [4].



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