recombinant human noggin 120-10c (PeproTech)

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PeproTech recombinant human noggin 120-10c
Recombinant Human Noggin 120 10c, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigma+plot+12%2E0+software/recombinant+human+noggin++120+10c/pmc11422091-76-83-88
Average 90 stars, based on 1 article reviews

recombinant human noggin 120-10c - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Pre-clinical evaluation of clinically relevant iPS cell derived neuroepithelial stem cells as an off-the-shelf cell therapy for spinal cord injury
Article Snippet: Briefly: iPS cells were plated on Lam521 (Biolamina, #LN521) coated tissue culture plates (dilute Lam521 1:20 in PBS) in Essential 8 medium and incubated at 37°C for 24 h. Medium was changed to knockout-serum-replacement (KOSR) medium (79% DMEM/F12+GlutaMax (Gibco, #31331-028), 1:5 KnockOut Serum Replacement (Gibco, #10828028), 1:100 Non-essential Amino Acids (Gibco, #11140-076), 1:550 2-mercapthoethanol (Gibco, #31350-010), 1:100 Penicillin-streptomycin (Gibco, #15140-122) supplemented fresh everyday by 10 μM of SB431542 (StemCell Technologies, #72232), 500 ng/mL of Noggin (PeproTech, #120-10C) and 3.3 μM CHIR (StemCell Technologies, #72052)).

Article Title: Personalized PDAC chip with functional endothelial barrier for tumour biomarker detection: A platform for precision medicine applications
Article Snippet: After 20 min 0.5 ml of organoid full medium containing AdDMEM/F12, 10,000 units/mL Pen/Strep, 40 % Wnt3A conditioned medium, 2 mM Glutamax, 10 mM Hepes, 1x B27 supplement (Gibco, #17504044), 1x N2 supplement (Gibco, #17502048), 1.25 mM N-acetylcystein (Sigma-Aldrich, #A7250), 10 mM Nicotinamide (Sigma-Aldrich, #240206), 10 nM Gastrin (Sigma-Aldrich, #G9145), 10 % RSPO conditioned medium (homemade), 50 ng/ml Noggin (Peprotech, #120-10C), 100 ng/ml FGF10 (Peprotech, #100-26), 50 ng/ml EGF, 1 μM Forskolin (Tocris, #1099), 0.5 μM A83-01 (Tocris, #2939), 50 μg/ml Primocin (InvivoGen, #ant-pm-2), 10 ng/ml FGF2 (Peprotech #100-18B) and Y27632 was added to wells and plate was placed back in the incubator.

Recombinant:

Article Title: Comprehensive protocols for culturing and molecular biological analysis of IBD patient-derived colon epithelial organoids.
Article Snippet: .. We add recombinant human Noggin (#120-10c Peprotech) to combined batches of Wnt3A and Rspondin1 enriched media and adjust media compositions to fit our experimental approaches. ..

Article Title: Protocol for the establishment and characterization of lung tumoroids from surgical resection without the use of Matrigel
Article Snippet: FGF-10 , STEMCELL Technologies , 78037. .. Recombinant human Noggin , PeproTech , 120-10C. ..

Article Title: Extrahepatic Bile Duct Organoids as a Model to Study Ischemia/Reperfusion Injury During Liver Transplantation
Article Snippet: Recovered cells were then suspended in Base Membrane Extract (BME; ref. #3533-010-02, R&D Systems, Bio-Techne, United States) and added to the culture plates. .. Supplemental start-up medium was added to the cultures consisting of nicotinamide (ref. #N0636, Sigma-Aldrich, Merck, DE), N-acetyl-L-cysteine (ref. #A9165, Sigma-Aldrich, Merck, DE), Y-27632 (ref. #1293823, biogems, United States), A 83-01 (ref. #9094360, biogems, United States), forskolin (ref. #1099, R&D Systems, Bio-Techne, United States), epidermal growth factor (ref. #AF-100-15, PeproTech, Thermo Fischer Scientific, DE), hepatocyte growth factor (ref. #100-39, PeproTech, Thermo Fischer Scientific, DE), fibroblast growth factor-10 (ref. #100-26, PeproTech, Thermo Fischer Scientific, DE), human [Leu 15 ]-gastrin I (ref. #G9145, Sigma-Aldrich, Merck, DE), recombinant human noggin (ref. #120-10C, PeproTech, Thermo Fischer Scientific, DE), recombinant human R-spondin-1 (ref. #120-38, PeproTech, Thermo Fischer Scientific, DE), Wnt3A (ref. #H17001, Sigma-Aldrich, Merck, DE), hES cell cloning & recovery supplement (ref. #01-0014-500, Stemolecule, Reprocell, JAP), B-27 supplement (ref. #12587010, Gibco, Thermo Fischer Scientific, DE) and N-2 supplement (ref. #17502001, Gibco, Thermo Fischer Scientific, DE). ..

Article Title: Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma.
Article Snippet: .. The medium for CSCC/ CAde-organoid consisted of advanced DMEM/f12 (Gibico, #12634010) supplemented with 1 × Hepes (Boster, #PYG0019), 1 × Penicillin/Streptomycin/ Amphotericin B, sterile solution (Solarbio, #P7630), 1 × Glutamax (Thermo Fisher Scientific, #35050061), 1x Mycoplasma Elimination Reagent (Yeasen, #40607ES03), 100 ng/ml Recombinant Human Noggin (PeproTech, #120-10C), 200 ng/ml Fibroblast growth factor 7 (PeproTech, #100-19), 100 ng/ml Fibroblast growth factor 10 (PeproTech, #100-26), 1 μM SB202190 (MCE, #HY-10295), 2.5 mM Nicotinamide (MCE, #HYB0150), 1.25 mM N-acetylcysteine (MCE, #HY-B0215), 10 μM Forskolin (MCE, #HY-15371), 1 × B-27 (Gibico, #17504044), 10 μM Y-27632 (MCE, #HY-10583), 500 nM A83-01 (Selleck, #S7692). .. The total RNA of primary fibroblasts was extracted using the Trizol reagent (Invitrogen, #15596026), and the cDNA was synthesized using the HiScript II Q RT Super Mix (Vazyme, #R223-01).

Article Title: Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma
Article Snippet: .. The medium for CSCC/CAde-organoid consisted of advanced DMEM/f12 (Gibico, #12634010) supplemented with 1 × Hepes (Boster, #PYG0019), 1 × Penicillin/Streptomycin/Amphotericin B, sterile solution (Solarbio, #P7630), 1 × Glutamax (Thermo Fisher Scientific, #35050061), 1x Mycoplasma Elimination Reagent (Yeasen, #40607ES03), 100 ng/ml Recombinant Human Noggin (PeproTech, #120-10C), 200 ng/ml Fibroblast growth factor 7 (PeproTech, #100-19), 100 ng/ml Fibroblast growth factor 10 (PeproTech, #100-26), 1 μM SB202190 (MCE, #HY-10295), 2.5 mM Nicotinamide (MCE, #HY-B0150), 1.25 mM N-acetylcysteine (MCE, #HY-B0215), 10 μM Forskolin (MCE, #HY-15371), 1 × B-27 (Gibico, #17504044), 10 μM Y-27632 (MCE, #HY-10583), 500 nM A83-01 (Selleck, #S7692). .. The total RNA of primary fibroblasts was extracted using the Trizol reagent (Invitrogen, #15596026), and the cDNA was synthesized using the HiScript II Q RT Super Mix (Vazyme, #R223-01).

Cloning:

Sterility:

Article Title: Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma
Article Snippet: .. The medium for CSCC/CAde-organoid consisted of advanced DMEM/f12 (Gibico, #12634010) supplemented with 1 × Hepes (Boster, #PYG0019), 1 × Penicillin/Streptomycin/Amphotericin B, sterile solution (Solarbio, #P7630), 1 × Glutamax (Thermo Fisher Scientific, #35050061), 1x Mycoplasma Elimination Reagent (Yeasen, #40607ES03), 100 ng/ml Recombinant Human Noggin (PeproTech, #120-10C), 200 ng/ml Fibroblast growth factor 7 (PeproTech, #100-19), 100 ng/ml Fibroblast growth factor 10 (PeproTech, #100-26), 1 μM SB202190 (MCE, #HY-10295), 2.5 mM Nicotinamide (MCE, #HY-B0150), 1.25 mM N-acetylcysteine (MCE, #HY-B0215), 10 μM Forskolin (MCE, #HY-15371), 1 × B-27 (Gibico, #17504044), 10 μM Y-27632 (MCE, #HY-10583), 500 nM A83-01 (Selleck, #S7692). .. The total RNA of primary fibroblasts was extracted using the Trizol reagent (Invitrogen, #15596026), and the cDNA was synthesized using the HiScript II Q RT Super Mix (Vazyme, #R223-01).

Review