gp120 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology gp120

(A) Representative immunoblot of <t>gp120</t> and tat protein in HIV-1 Tg rat brain (A), detected as described in Methods . mRNA levels of brain GFAP (B) and CD11b (D) in control and HIV-1 Tg rat brain, measured using real time RT-PCR, normalized to β-globulin and relative to control level (calibrator) using the ΔΔC T method. Representative immunoblots of (C) GFAP and (E) CD11b protein in control and HIV-1 Tg rat brain. Bar graphs are ratios of optical densities of individual protein bands to β-actin, expressed as percent of control. Data represent mean ± SEM, statistical significance: **p < 0.01, ***p < 0.001 as determined by unpaired t-test.

Gp120, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigma+plot+12%2E0+software/HIV-1+gp120+Antibody/pmc03175175-52-62-70
Average 93 stars, based on 24 article reviews

gp120 - by Bioz Stars, 2026-11

93/100 stars

Images

1) Product Images from "Increased neuroinflammatory and arachidonic acid cascade markers, and reduced synaptic proteins, in brain of HIV-1 transgenic rats"

Article Title: Increased neuroinflammatory and arachidonic acid cascade markers, and reduced synaptic proteins, in brain of HIV-1 transgenic rats

Journal: Journal of Neuroinflammation

doi: 10.1186/1742-2094-8-101

(A) Representative immunoblot of gp120 and tat protein in HIV-1 Tg rat brain (A), detected as described in Methods . mRNA levels of brain GFAP (B) and CD11b (D) in control and HIV-1 Tg rat brain, measured using real time RT-PCR, normalized to β-globulin and relative to control level (calibrator) using the ΔΔC T method. Representative immunoblots of (C) GFAP and (E) CD11b protein in control and HIV-1 Tg rat brain. Bar graphs are ratios of optical densities of individual protein bands to β-actin, expressed as percent of control. Data represent mean ± SEM, statistical significance: **p < 0.01, ***p < 0.001 as determined by unpaired t-test.

Figure Legend Snippet: (A) Representative immunoblot of gp120 and tat protein in HIV-1 Tg rat brain (A), detected as described in Methods . mRNA levels of brain GFAP (B) and CD11b (D) in control and HIV-1 Tg rat brain, measured using real time RT-PCR, normalized to β-globulin and relative to control level (calibrator) using the ΔΔC T method. Representative immunoblots of (C) GFAP and (E) CD11b protein in control and HIV-1 Tg rat brain. Bar graphs are ratios of optical densities of individual protein bands to β-actin, expressed as percent of control. Data represent mean ± SEM, statistical significance: **p < 0.01, ***p < 0.001 as determined by unpaired t-test.

Techniques Used: Western Blot, Control, Quantitative RT-PCR

Binding Assay:

Article Title: Adeno-associated virus delivery of anti-HIV monoclonal antibodies can drive long-term virologic suppression
Article Snippet: .. Antibodies binding to denatured gp120 were detected with a goat anti-monkey secondary antibody (Santa Cruz). ..

Incubation:

Article Title: Potent Induction of Envelope-Specific Antibody Responses by Virus-Like Particle Immunogens Based on HIV-1 Envelopes from Patients with Early Broadly Neutralizing Responses
Article Snippet: .. Membranes were then incubated with antibodies recognizing the gp120 (447-52D provided by the NIH AIDS Research and Reference Reagent Program) and Gag (p24 and p55) (p24 Antibody [24-4]; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. A goat anti-human horseradish peroxidase (HRP) (catalog no. sc-2907; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and a chicken anti-mouse-HRP (catalog no. sc-2954; Santa Cruz Biotechnology, CA, USA) was used to detect gp120 and p55.

Article Title: Variations in Env at amino acids 328 and 330 affect HIV-1 replicative fitness and entry inhibitor sensitivity.
Article Snippet: While the variations in the HIV-1 Env V3 loop have been the focus of much research to explore its functional effect, how specific mutations of certain amino acids in the V3 loop affect viral fitness remains unclear.. In this study, we evaluated a series of natural polymorphisms at positions 328 and 330 with different combinations of adjacent glycosylation sites in the HIV-1 subtype B backbone to address their role in replicative fitness and sensitivity to entry inhibitors based on analysis of env sequence frequency at the population level.. Pairwise growth competition experiment showed that wild-type virus with major consensus amino acids obviously had higher replicative fitness (P < 0.001).

Article Title: Phospho-Chitooligosaccharides below 1 kDa Inhibit HIV-1 Entry In Vitro
Article Snippet: .. Briefly, wells of an ELISA plate were coated with 2 μg/mL anti-gp120 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) in carbonate buffer (pH 9.6) and incubated overnight at 4 ° C. Wells were then blocked with PBS containing 0.1% BSA at 37 °C for 1 h. Recombinant HIV-1 IIIB (100 ng/well) in PBS was added to each well, and the plate was incubated at 37 ° C for 1 h. The wells were then washed with PBS containing 0.05% Tween 20. ..

Article Title: Guiding the humoral response against HIV-1 toward a MPER adjacent region by immunization with a VLP-formulated antibody-selected envelope variant
Article Snippet: .. Membranes were then incubated with antibodies recognizing the gp120 (447-52D provided by the NIH AIDS Research and Reference Reagent Program), Gag (p24 and p55) [p24 Antibody (24–4); Santa Cruz Biotechnology, Santa Cruz, CA, USA] and β-actin [β-Actin (13E5) Rabbit mAb; Cell Signaling Technology, Danvers, MA, U.S.A.). .. A goat anti-human-HRP (SC-2907; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used to detect gp120.

Enzyme-linked Immunosorbent Assay:

Recombinant:

Gentle:

Article Title: Retargeting Oncolytic Vesicular Stomatitis Virus to Human T-Cell Lymphotropic Virus Type 1-Associated Adult T-Cell Leukemia
Article Snippet: .. Membranes were blocked with 5% milk powder in PBS– 0.1% Tween 20 at room temperature and then probed with antibodies against HIV gp41 (ARRRP 2F5, 1:5,000; PBS-Tween, 0.1%), HIV gp120 (Santa Cruz, 1:5,000; PBS-Tween, 0.1%), VSV-G (Sigma, 1:10,000; 5% milk in 0.1% PBS-Tween), or -actin (Sigma, 1:20,000; 5% milk in 0.1% PBSTween) overnight at 4°C with gentle rocking. .. The membranes were probed with horseradish peroxidase-conjugated anti-human, anti-goat, or anti-mouse antibody (Santa Cruz) diluted 1:10,000 in blocking buffer.

Immunoprecipitation:

Article Title: SERINC5 Inhibits HIV-1 Infectivity by Altering the Conformation of gp120 on HIV-1 Particles
Article Snippet: .. Proteins were immunoprecipitated by overnight 381 rotation at 4°C in 0.3 mL of solution containing 20 mL of a 1:1 slurry of Protein A/G+ 382 beads (Santa Cruz, Inc.) that had been pre-bound to monoclonal and polyclonal 383 antibodies specific for gp120 and/or gp41. ..

Article Title: SERINC5 Inhibits HIV-1 Infectivity by Altering the Conformation of gp120 on HIV-1 Particles
Article Snippet: .. Proteins were immunoprecipitated by overnight rotation at 4°C in 0.3 ml of solution containing 20 ml of a 1:1 slurry of protein A/G+ beads (Santa Cruz, Inc.) that had been prebound to monoclonal and polyclonal antibodies specific for gp120 and/or gp41. ..

Review