Cell Culture:Article Title: Depletion of tropomyosin 1.6 alleviates TGF-β1-induced fibroblast activation by promoting the α-SMA-MMP9 matrix-degrading structure
Article Snippet: The grid with samples was blocked for 30 min, then incubated with an antibody against α-SMA (Sigma-Aldrich; Cat# A5228) for 2 h, followed by incubation with a nanogold-conjugated secondary antibody for 1 h. To enhance the contrast of the sections, grids were stained with uranyl acetate and lead citrate and the resulting samples were then investigated by a JEM1400 electron transmission microscope to observe the structure features of the scaffold and α-SMA-positive aggregates at high resolution. .. NRK-49F cells or NRK-49F cells harboring RNA interference were cultured on FITC-conjugated collagen gel with or without TGF-β1 induction for 24 h and co-treated with DMSO, blebbistatin (actomyosin blocker, 20 μM; Tocris), and GM6001 (pan-MMP inhibitor, 20 μM; Sigma-Aldrich). ..
Article Title: Unveiling Therapeutic Opportunities with Melanoma Patient-derived Organoid Models.
Article Snippet: With the development of immunotherapy, there is an ongoing need to develop models that can recapitulate the tumor microenvironment of native tumors.. While traditional twoand three-dimensional models can offer insights into cancer development and progression, these lack crucial aspects that hinder a faithful mimic of native tumors.. An alternative model that has gained a lot of attention is the patient-derived organoid.
Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
Sterility:Article Title: Unveiling Therapeutic Opportunities with Melanoma Patient-derived Organoid Models.
Article Snippet: With the development of immunotherapy, there is an ongoing need to develop models that can recapitulate the tumor microenvironment of native tumors.. While traditional twoand three-dimensional models can offer insights into cancer development and progression, these lack crucial aspects that hinder a faithful mimic of native tumors.. An alternative model that has gained a lot of attention is the patient-derived organoid.
Staining:Article Title: Unveiling Therapeutic Opportunities with Melanoma Patient-derived Organoid Models.
Article Snippet: With the development of immunotherapy, there is an ongoing need to develop models that can recapitulate the tumor microenvironment of native tumors.. While traditional twoand three-dimensional models can offer insights into cancer development and progression, these lack crucial aspects that hinder a faithful mimic of native tumors.. An alternative model that has gained a lot of attention is the patient-derived organoid.
Incubation:Article Title: Development of a genetically encoded melanocortin sensor for high sensitivity intravital imaging
Article Snippet: After blocking, sections were incubated in primary antibody (Aves anti-GFP cat#GFP-1020; Thermo Fisher, Waltham, MA, USA) in PBST for 24 hours at room temperature. .. The following day, tissues were washed three times with PBS and incubated for two hours with secondary antibody (goat anti-chicken FITC; Jackson Immunoresearch, Baltimore Pike, PA, USA) with DAPI (Tocris Bioscience, Bristol, UK). .. Tissues were then washed three times, mounted onto glass slides, mounting media and coverslips applied and imaged on an Olympus BX61.
Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
Recombinant:Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
Modification:Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
Stable Transfection:Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
Expressing:Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
Transferring:Article Title: Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER odies 4 (E2A73) Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 His6 Abcam Cat# ab18184; RRID: AB_444306 GST Abcam Cat# ab19256; RRID: AB_444809 rabbit Nano-Secondary (CTK0101) ChromoTek Cat# shurbGNHS-1; RRID: AB_2864267 GST VHH ChromoTek Cat# ST-250; RRID: AB_2631368 GFP VHH ChromoTek Cat# GT-250; RRID: AB_2631361 icals, peptides, and recombinant proteins ecco’s modified Eagle’s medium (DMEM) Genesee Scientific Cat# 25-500 sin-EDTA Fisher Scientific Cat# 25200114 l bovine serum (FBS) Peak Serum Cat# PS-FB2 cillin-streptomycin (1003) Lonza Cat# 17-602E Fluor-1-Pfp Payne et al.2 N/A Fluor-1-Pfp Tocris Cat# 7920 8-Tfp Thermo Fisher Scientific Cat# A37570 CRBN/DDB1 Bristol Myers Squibb Also see Lin, W. et al. for compatible construct6 A generous gift FITC Ichikawa et al.1 N/A BRD4(BD1) BPS Cat# 31040 BRD4(BD2) EpiCypher Cat# 15-0013 FITC Payne et al.4 N/A FITC Tocris Cat# 7722 FKBP12 Ichikawa et al.1 N/A FITC Ichikawa et al.1 N/A GST-CDK4/GST-CD3 BPS Cat# 40104 CDK6/GST-CD1 BPS Cat# 40097 ociclib-FITC Ichikawa et al.1 N/A F hydrochloride Combi Blocks Cat# SS-7834 onase nuclease MilliporeSigma Cat# E1014 Thermo Fisher Scientific Cat# R0861 lidomide-50-NH2 Muller et al. 7 N/A rimental models: Cell lines -MB-231 ATCC Cat# HTB-26 293T cells stably expressing stably expressing BRD4(BD1)-GFP Nowak et al.8 N/A 293T cells stably expressing stably expressing BRD4(BD2)-GFP Nowak et al.8 N/A are and algorithms osoft Excel (v16.44) Microsoft N/A hPad Prism 10 GraphPad Software N/A kControl V2.1 Tecan Group Ltd. N/A r k multimode microplate reader (monochromator) Tecan Group Ltd. N/A e 384-well plates for TR-FRET Corning Cat# 3572 e 384-well plates for TR-FRET Greiner Cat# 781207 e low-volume 384-well plates for TR-FRET Revvity Cat# 6008280 ell plates for cell culture Corning Cat# 3904 rbug incubated microplate shaker Boekel Scientific Cat# 130000 annel, 15–1,250 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2034 (Continued on next page) STAR Protocols 5, 103129, June 21, 2024 Continued REAGENT or RESOURCE SOURCE IDENTIFIER 12-channel, 2–125 mL Matrix Equalizer electronic multichannel pipette Thermo Fisher Scientific Cat# 2231 Multidrop Combi reagent dispenser Thermo Fisher Scientific Cat# 5840300 7K MWCO Zeba spin desalting columns, 0.5 mL Thermo Fisher Scientific Cat# 89883 ll OPEN ACCESSProtocol ..
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