Microscopy:Article Title: Channelrhodopsin variants for high-rate optogenetic neurostimulation at low light intensities.
Article Snippet: Andor LASX_Office (1.4.3) Leica, Hamburg, Germany Custom graphical interface Napari Cheetah recording software Neuralynx Other Amicon filters EMD UFC910024 AAV titration kit TaKaRa/Clontech 6233 Naica Prism3 reader Stilla Axopatch 200B amplifier Axon Instruments, Union City, USA DigiData 1320 A interface Axon Instruments, Union City, USA Pipette puller (Model P1000) Sutter Instruments, Novato, USA Diode-pumped solid-state lasers (λ= 473 nm) Changchun New Industries Optoelectronics Tech. .. Co., Ltd., Changchun, China Fast computer-controlled shutter Uniblitz LS6ZM2 VincentAssociates, Rochester, USA Opolette 355 tunable laser system Opotek Inc., Carlsbad, USA Diode-pumped solid-state laser (LBX-488-40-CSB; 488 nm 40mW,) Oxxius Coherent Inc Upright Olympus BX51WI microscope with a x40/0.8 LUMPLFLN40XW objective Olympus Andor zyla sCMOS camera Andor mCherry HC Filter Set mirror (F36-508 HC-Set mCherry) AHF - IDEX/Semrock EGFP HC Filter Set mirror (F36-528 HC-Set EGFP) AHF - IDEX/Semrock EPC10 USB HEKA patch clamp amplifier Harvard Bioscience Inc. FieldMaxII-TOP laser power meter Coherent Temperature controller SC20 and CL-200A Warner Instruments Falcon cell strainer (100 μm) Corning 352360 VT 1200 vibratome Leica Microsystems, Wetzlar, Germany Borosilicate glass capillaries with filament (GB150F, 0.86 × 1.50 × 80 mm) Science Products, 154 Hofheim, Germany Imaging spacer Grace BioLabs SecureSeal TM (GBL654008-100EA) Sigma-Aldrich Optical fiber (200-μm diameter, 0.39 NA) Thorlabs 473 nm laser (MLLFN-473100) Changchun New Industry Optoelectronics Reagent/resource Reference or source Identifier or catalog number 488 nm laser (LBX-488-100) Oxxius Coherent Inc Laser power meter (Solo-2; PM103USB; S140C). .. Gentec-EO and Thorlabs National Instrument data acquisition cards (NI PCI6229) National Instruments Soundproof chamber Industrial Acoustics Loudspeaker (Scanspeak Ultrasound; Vifa) Avisoft Bioacoustics 0.25-inch microphone (4039; 46BF-1) Brüel & Kjaer, GRAS Preamplifier (12AQ) GRAS 32-channel linear silicone probe (A1x32-6mm-50-177A32, 50-μm thickness) NeuroNexus Digital Lynx 4S recording system Neuralynx LED chips (C460TR2227S2100) Cree Confocal microscope SP8 Leica, Hamburg, Germany Micromanipulator (SM-10 compact) Luigs & Neumann LEICA CM3050 S Crysotat Leica Biosystems, Nussloch GmbH 2025 Automated immunostaining system - Discovery XT Roche Diagnostics GmbH, Mannheim, Germany)
Patch Clamp:Article Title: Channelrhodopsin variants for high-rate optogenetic neurostimulation at low light intensities.
Article Snippet: Andor LASX_Office (1.4.3) Leica, Hamburg, Germany Custom graphical interface Napari Cheetah recording software Neuralynx Other Amicon filters EMD UFC910024 AAV titration kit TaKaRa/Clontech 6233 Naica Prism3 reader Stilla Axopatch 200B amplifier Axon Instruments, Union City, USA DigiData 1320 A interface Axon Instruments, Union City, USA Pipette puller (Model P1000) Sutter Instruments, Novato, USA Diode-pumped solid-state lasers (λ= 473 nm) Changchun New Industries Optoelectronics Tech. .. Co., Ltd., Changchun, China Fast computer-controlled shutter Uniblitz LS6ZM2 VincentAssociates, Rochester, USA Opolette 355 tunable laser system Opotek Inc., Carlsbad, USA Diode-pumped solid-state laser (LBX-488-40-CSB; 488 nm 40mW,) Oxxius Coherent Inc Upright Olympus BX51WI microscope with a x40/0.8 LUMPLFLN40XW objective Olympus Andor zyla sCMOS camera Andor mCherry HC Filter Set mirror (F36-508 HC-Set mCherry) AHF - IDEX/Semrock EGFP HC Filter Set mirror (F36-528 HC-Set EGFP) AHF - IDEX/Semrock EPC10 USB HEKA patch clamp amplifier Harvard Bioscience Inc. FieldMaxII-TOP laser power meter Coherent Temperature controller SC20 and CL-200A Warner Instruments Falcon cell strainer (100 μm) Corning 352360 VT 1200 vibratome Leica Microsystems, Wetzlar, Germany Borosilicate glass capillaries with filament (GB150F, 0.86 × 1.50 × 80 mm) Science Products, 154 Hofheim, Germany Imaging spacer Grace BioLabs SecureSeal TM (GBL654008-100EA) Sigma-Aldrich Optical fiber (200-μm diameter, 0.39 NA) Thorlabs 473 nm laser (MLLFN-473100) Changchun New Industry Optoelectronics Reagent/resource Reference or source Identifier or catalog number 488 nm laser (LBX-488-100) Oxxius Coherent Inc Laser power meter (Solo-2; PM103USB; S140C). .. Gentec-EO and Thorlabs National Instrument data acquisition cards (NI PCI6229) National Instruments Soundproof chamber Industrial Acoustics Loudspeaker (Scanspeak Ultrasound; Vifa) Avisoft Bioacoustics 0.25-inch microphone (4039; 46BF-1) Brüel & Kjaer, GRAS Preamplifier (12AQ) GRAS 32-channel linear silicone probe (A1x32-6mm-50-177A32, 50-μm thickness) NeuroNexus Digital Lynx 4S recording system Neuralynx LED chips (C460TR2227S2100) Cree Confocal microscope SP8 Leica, Hamburg, Germany Micromanipulator (SM-10 compact) Luigs & Neumann LEICA CM3050 S Crysotat Leica Biosystems, Nussloch GmbH 2025 Automated immunostaining system - Discovery XT Roche Diagnostics GmbH, Mannheim, Germany)
Imaging:Article Title: Channelrhodopsin variants for high-rate optogenetic neurostimulation at low light intensities.
Article Snippet: Andor LASX_Office (1.4.3) Leica, Hamburg, Germany Custom graphical interface Napari Cheetah recording software Neuralynx Other Amicon filters EMD UFC910024 AAV titration kit TaKaRa/Clontech 6233 Naica Prism3 reader Stilla Axopatch 200B amplifier Axon Instruments, Union City, USA DigiData 1320 A interface Axon Instruments, Union City, USA Pipette puller (Model P1000) Sutter Instruments, Novato, USA Diode-pumped solid-state lasers (λ= 473 nm) Changchun New Industries Optoelectronics Tech. .. Co., Ltd., Changchun, China Fast computer-controlled shutter Uniblitz LS6ZM2 VincentAssociates, Rochester, USA Opolette 355 tunable laser system Opotek Inc., Carlsbad, USA Diode-pumped solid-state laser (LBX-488-40-CSB; 488 nm 40mW,) Oxxius Coherent Inc Upright Olympus BX51WI microscope with a x40/0.8 LUMPLFLN40XW objective Olympus Andor zyla sCMOS camera Andor mCherry HC Filter Set mirror (F36-508 HC-Set mCherry) AHF - IDEX/Semrock EGFP HC Filter Set mirror (F36-528 HC-Set EGFP) AHF - IDEX/Semrock EPC10 USB HEKA patch clamp amplifier Harvard Bioscience Inc. FieldMaxII-TOP laser power meter Coherent Temperature controller SC20 and CL-200A Warner Instruments Falcon cell strainer (100 μm) Corning 352360 VT 1200 vibratome Leica Microsystems, Wetzlar, Germany Borosilicate glass capillaries with filament (GB150F, 0.86 × 1.50 × 80 mm) Science Products, 154 Hofheim, Germany Imaging spacer Grace BioLabs SecureSeal TM (GBL654008-100EA) Sigma-Aldrich Optical fiber (200-μm diameter, 0.39 NA) Thorlabs 473 nm laser (MLLFN-473100) Changchun New Industry Optoelectronics Reagent/resource Reference or source Identifier or catalog number 488 nm laser (LBX-488-100) Oxxius Coherent Inc Laser power meter (Solo-2; PM103USB; S140C). .. Gentec-EO and Thorlabs National Instrument data acquisition cards (NI PCI6229) National Instruments Soundproof chamber Industrial Acoustics Loudspeaker (Scanspeak Ultrasound; Vifa) Avisoft Bioacoustics 0.25-inch microphone (4039; 46BF-1) Brüel & Kjaer, GRAS Preamplifier (12AQ) GRAS 32-channel linear silicone probe (A1x32-6mm-50-177A32, 50-μm thickness) NeuroNexus Digital Lynx 4S recording system Neuralynx LED chips (C460TR2227S2100) Cree Confocal microscope SP8 Leica, Hamburg, Germany Micromanipulator (SM-10 compact) Luigs & Neumann LEICA CM3050 S Crysotat Leica Biosystems, Nussloch GmbH 2025 Automated immunostaining system - Discovery XT Roche Diagnostics GmbH, Mannheim, Germany)
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