lentivirus expressing shrna constructs (Merck & Co)
86
Structured Review
Merck & Co
lentivirus expressing shrna constructs
Lentivirus Expressing Shrna Constructs, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+construct+shctrl/expressing+hairpin+lentiviral+mission+particles+rna+short+shrna+shrna+sirt3+targeting+transduction+trc/pmc12325171-221-19-30
Average 86 stars, based on 1 article reviews
Lentivirus Expressing Shrna Constructs, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+construct+shctrl/expressing+hairpin+lentiviral+mission+particles+rna+short+shrna+shrna+sirt3+targeting+transduction+trc/pmc12325171-221-19-30
Average 86 stars, based on 1 article reviews
lentivirus expressing shrna constructs - by Bioz Stars,
2026-10
86/100 stars
Images
1) Product Images from "The LRP4/YAP axis drives the radiation-tolerant persister (RTP) cell state in breast cancer"
Article Title: The LRP4/YAP axis drives the radiation-tolerant persister (RTP) cell state in breast cancer
Journal: Theranostics
doi: 10.7150/thno.101393
Figure Legend Snippet: A RNAi screen for the identification of functional regulator of radiation-induced cell plasticity. A . schematic representation of RNAi screening strategy. B-C . Proportion of ALDH br cells in BFP + ( B ) and RFP + SUM159 cells ( C ) following silencing of each individual gene contained in the RNAi library and normalized with non-targeting siRNA (siCTRL). Genes targeted by a siRNA inducing a significant reduction of the ALDH br cell proportion are highlighted. Statistical test used is Student's t-test. Data represent mean ± SD D . Representative images of the high-content screening captures. ALDEFLUOR cellular staining is represented in green. Dead cells are labeled by DRAQ5 in red. RFP+ cells are in yellow and BFP+ cells in blue. E-F . Validation of candidate genes using FACS analysis. Proportion of ALDH br cells in BFP + ( E ) and RFP + SUM159 cells ( F ) are represented normalized with non-targeting siRNA (siCTRL). siRNA targeting ALDH1A1 was used as positive control and siRNA targeting TEXT12 or VDACL were used as negative control. Statistical test used is Student's t-test. Data represent mean ± SD. *p<0.05, **p<0.01, ***p<0.001.
Techniques Used: Functional Assay, High Content Screening, Staining, Labeling, Biomarker Discovery, Positive Control, Negative Control
Figure Legend Snippet: LRP4/YAP axis inhibition radiosensitizes iCSC. A-B . Proportion of cells ( A , SUM159; B , S68) presenting a nuclear location of YAP in each cell subpopulations five days post-irradiation, with representative images of YAP staining (in green) in each cell subpopulations on the right panel. Nuclei are counterstained with DAPi (in blue). C . Pre-ranked GSEA interrogating differential expression between iCSC and late non-CSC and YAP/TAZ target genes. D . Western blot of markers related to YAP/TAZ signaling and its activation in SUM159 and S68 cells silenced for LRP4 (shLRP4) compared to the non-targeting shRNA (shCTRL). The mean intensities are indicated below each band for each condition. E . Heat map representing the mRNA expression of the LRP4, ALDH1A1, and YAP/TAZ target genes in SUM159 and S68 silenced for LRP4 (siLRP4) compared to the non-targeting siRNA (siCTRL). Each row represents three independent replicates per conditions (R1, R2, and R3). F . Proportion of SUM159 cells presenting a nuclear location of YAP in late non-CSC and iCSC following LRP4 silencing (siLRP4) compared to a non-targeting siRNA (siCTRL). G-H . SUM159 ( G ) and S68 cells ( H ), following LRP4 silencing (shLRP4) compared to a non-targeting siRNA (shCTRL), were exposed to various dose of radiation therapy and subjected to clonogenic survival assays ( G ), with representative images (right panels). I . Patient-derived xenograft organoid (PDXO) size distribution for CRCM389 cells WT (shCTRL) or silenced for LRP4 (shLRP4) following irradiation (RT) and compared to untreated condition (CTRL) (left panel). Representative pictures of PDXO-CRCM389 7 days post-treatment (right panel). Statistical test used is Student's t-test. Data represent mean ± SD. ns (not significant), *p<0.05, **p<0.01, ***p<0.001. J . Kaplan-Meier tumor-free survival curves of mice xenografted with 100,000-200,000 SUM159 irradiated cells silenced for LRP4 (shLRP4) compared to the control (shCTRL). p-value and hazard ration (HR) estimated according to Log-rank (Mantel-Cox) test.
Techniques Used: Inhibition, Irradiation, Staining, Quantitative Proteomics, Western Blot, Activation Assay, shRNA, Expressing, Derivative Assay, Control
Related Articles
Generated:Article Title: Filopodia-mediated trans-endocytosis Article Snippet: HUVECs were electroporated using the Neon Transfection System (ThermoFisher Scientific, MPK5000) with the 10 μL kit (MPK1025; Thermo Fisher Scientific) following the manufacturer’s instructions (150,000 cells and 1 μg plasmid DNA per transfection) and as previously described ( ). .. The DCIS.com lifeact-RFP shCTRL and shMYO10 lines were generated previously ( ) using lentiviral particles containing a Control:Article Title: Filopodia-mediated trans-endocytosis Article Snippet: HUVECs were electroporated using the Neon Transfection System (ThermoFisher Scientific, MPK5000) with the 10 μL kit (MPK1025; Thermo Fisher Scientific) following the manufacturer’s instructions (150,000 cells and 1 μg plasmid DNA per transfection) and as previously described ( ). .. The DCIS.com lifeact-RFP shCTRL and shMYO10 lines were generated previously ( ) using lentiviral particles containing a Article Title: Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers Article Snippet: FANCA shRNA knockdown (KD) was performed in RPE1 hTERT p53 -/- cells using two FANCA -targeting shRNAs (TRCN0000296799 and TRCN0000291182, Merck). .. Additionally, one Article Title: Heme oxygenase-1 emerges as a critical regulator linking autophagy dysregulation, immune infiltration, and NOD-Like receptor signaling in PCOS pathogenesis. Article Snippet: Polycystic ovary syndrome (PCOS) is a prevalent endocrine-metabolic disorder affecting approximately 6–10% of women of reproductive age [1].. It is primarily characterized by hyperandrogenism, chronic anovulation, and polycystic ovarian morphology, all of which contribute to female infertility due to anovulation and hyperandrogenism [2].. Additionally, most PCOS patients develop peripheral insulin resistance (IR), increasing the risk of type 2 diabetes (DM2). shRNA:Article Title: Filopodia-mediated trans-endocytosis Article Snippet: HUVECs were electroporated using the Neon Transfection System (ThermoFisher Scientific, MPK5000) with the 10 μL kit (MPK1025; Thermo Fisher Scientific) following the manufacturer’s instructions (150,000 cells and 1 μg plasmid DNA per transfection) and as previously described ( ). .. The DCIS.com lifeact-RFP shCTRL and shMYO10 lines were generated previously ( ) using lentiviral particles containing a Article Title: Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers Article Snippet: FANCA shRNA knockdown (KD) was performed in RPE1 hTERT p53 -/- cells using two FANCA -targeting shRNAs (TRCN0000296799 and TRCN0000291182, Merck). .. Additionally, one Article Title: Heme oxygenase-1 emerges as a critical regulator linking autophagy dysregulation, immune infiltration, and NOD-Like receptor signaling in PCOS pathogenesis. Article Snippet: Polycystic ovary syndrome (PCOS) is a prevalent endocrine-metabolic disorder affecting approximately 6–10% of women of reproductive age [1].. It is primarily characterized by hyperandrogenism, chronic anovulation, and polycystic ovarian morphology, all of which contribute to female infertility due to anovulation and hyperandrogenism [2].. Additionally, most PCOS patients develop peripheral insulin resistance (IR), increasing the risk of type 2 diabetes (DM2). other:Article Title: Spatiotemporal regulation of energetic charge dictates T cell function Article Snippet: T cells were activated in plates coated with anti-CD3 monoclonal antibody (mAb) (2.5 μg ml−1; clone 17.A2; BioLegend) in the presence of soluble anti-CD28 mAb (2.5 μg ml−1; clone 37.51; BioLegend) and murine IL-12 (10 ng ml−1; I8523; Sigma-Aldrich) . Article Title: Spatiotemporal regulation of energetic charge dictates T cell function. Article Snippet: Generation of T cells expressing the glucose reporter iGluco The construct of encoding the iGlucoSnFR-mRuby2 (iGluco) glucose probe17 was obtained from Addgene and subcloned into the retroviral vector MSCV. Incubation:Article Title: SARS-CoV-2 nonspike structural proteins hijack mucosa epithelial cell fate. Article Snippet: .. For full thickness 3D equivalents, samples were incubated with 200 μl Article Title: SARS-CoV-2 nonspike structural proteins hijack mucosa epithelial cell fate Article Snippet: .. For full thickness 3D equivalents, samples were incubated with 200 μl |
