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shrna construct shctrl  (Thermo Fisher)


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    Structured Review

    Thermo Fisher shrna construct shctrl
    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based <t>shRNA</t> silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of <t>normalized</t> <t>shCTRL</t> samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
    Shrna Construct Shctrl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+construct+shctrl/lentiviral+shrna+pgipz+vectors+ns+rhs4348/pmc08235627-77-1-26
    Average 90 stars, based on 1 article reviews
    shrna construct shctrl - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells"

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells

    Journal: Cancers

    doi: 10.3390/cancers13123055

    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
    Figure Legend Snippet: Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Techniques Used: Western Blot, shRNA, MANN-WHITNEY, Marker

    Related Articles

    shRNA:

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells
    Article Snippet: .. Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific. ..

    Article Title: Autocrine VEGFR1 and VEGFR2 signaling promotes survival in human glioblastoma models in vitro and in vivo
    Article Snippet: .. The nonsilencing control (#RHS4348) and the silencing microRNA-adapted shRNA ( shRNAmir ) pGIPZ lentiviral vectors (#RHS4531-V3LHS_403557; #RHS4531-V3LHS_302174) containing a single VEGFA (TCTGTATCGATCGTTCTGT) or VEGFR1-targeting hairpin sequence (TGAACCTGAACTAGATCCT) provided in bacterial stocks of E. coli were purchased from Thermo Scientific Open Biosystems. ..

    Article Title: Silencing KRAS Overexpression in Cadmium-Transformed Prostate Epithelial Cells Mitigates Malignant Phenotype
    Article Snippet: Keratinocyte-serum free medium (K-SFM), bovine pituitary extract (BPE), epidermal growth factor (EGF), and 100X antibiotic-antimycotic mixture were purchased from Life Technologies, Inc. (Grand Island, NY). .. GIPZ lentiviral KRAS shRNAmir particles (catalog no. VGH5523, clone ID: V3LHS_314009), and nonsilencing negative control shRNA (catalog no. RHS4348) were purchased from Thermo Fisher Scientific (Lafayette, CO). .. Puromycin was purchased from Cellgro (Manassas, VA).

    Article Title: NFκB-Mediated Invasiveness in CD133+ Pancreatic TICs Is Regulated by Autocrine and Paracrine Activation of IL1 Signaling
    Article Snippet: Human cDNA empty vector plasmid (EX-NEG-M02) and CD133 expression plasmid (EXZ0396-M02) were attained from GeneCopoeia. .. Lentiviral shRNA pGIPZ vectors; NS (RHS4348) and αCD133 (V2LHS_71816) were acquired from Thermo Scientific. .. NF-kB activity was inhibited after transfecting cells with pBabe-puro-IKBalphamut suprerepressor (a gift from William Hahn [ Addgene plasmid#15291].

    Construct:

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells
    Article Snippet: .. Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific. ..

    Plasmid Preparation:

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells
    Article Snippet: .. Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific. ..

    Sequencing:

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells
    Article Snippet: .. Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific. ..

    Article Title: Autocrine VEGFR1 and VEGFR2 signaling promotes survival in human glioblastoma models in vitro and in vivo
    Article Snippet: .. The nonsilencing control (#RHS4348) and the silencing microRNA-adapted shRNA ( shRNAmir ) pGIPZ lentiviral vectors (#RHS4531-V3LHS_403557; #RHS4531-V3LHS_302174) containing a single VEGFA (TCTGTATCGATCGTTCTGT) or VEGFR1-targeting hairpin sequence (TGAACCTGAACTAGATCCT) provided in bacterial stocks of E. coli were purchased from Thermo Scientific Open Biosystems. ..

    Control:

    Article Title: Autocrine VEGFR1 and VEGFR2 signaling promotes survival in human glioblastoma models in vitro and in vivo
    Article Snippet: .. The nonsilencing control (#RHS4348) and the silencing microRNA-adapted shRNA ( shRNAmir ) pGIPZ lentiviral vectors (#RHS4531-V3LHS_403557; #RHS4531-V3LHS_302174) containing a single VEGFA (TCTGTATCGATCGTTCTGT) or VEGFR1-targeting hairpin sequence (TGAACCTGAACTAGATCCT) provided in bacterial stocks of E. coli were purchased from Thermo Scientific Open Biosystems. ..

    other:

    Article Title: Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain.
    Article Snippet: For injection of shRNA-lentiviral vectors, 3 ml of lentiviral vector for Sh-RNA MARCKS (sc35858-V, Santa Cruz, TX, USA; 1 × 106 TU), Sh-RNA SPTA2 (mixture ofV3LMM_423737, V2LMM_104859 and V2LMM_ 202472, Thermo, MA, USA; 1 × 108 TU) or scrambled shRNA (RHS4348, Thermo Scientific, MA, USA; 1 × 106 TU) was injected to same region as the AAV1-EGFP.

    Article Title: Elevation of p11 in lateral habenula mediates depression-like behavior
    Article Snippet: For gene silencing, Lenti-GFPshRNAmir (GFP-shRNA, RHS4348) and Lenti-p11-GFP-shRNAmir (p11-shRNA, VGM5524-99213741) viruses were purchased from Thermo Scientific (Waltham, MA, USA).

    Article Title: Cellular and molecular basis for stress-induced depression
    Article Snippet: For gene silencing, Lenti-GFP-shRNAmir (GFP-shRNA, RHS4348) and Lenti-p11-GFP-shRNAmir (p11-shRNA, VGM5524-99213741) viruses were purchased from Thermo Scientific (Waltham, MA, USA).

    Article Title: Knockdown of dual-specificity phosphatase 3 drives differentiation and polarization of myeloid leukemia cells into macrophages with reduced proliferative and DNA repair fitness.
    Article Snippet: Dual-specificity phosphatase 3 (DUSP3) regulates key cellular processes, including the cell cycle, proliferation, and differentiation.. Recently, we demonstrated its crucial role in maintaining genomic stability by interacting with and dephosphorylating nucleophosmin (NPM), thereby modulating nuclear p53 activity under genotoxic stress.. Given the frequent mutations in both p53 and NPM in acute myeloid leukemia (AML), this study aimed to investigate the impact of DUSP3 knockdown in two p53-deficient AML cell lines and explore potential correlations with NPM expression.

    Negative Control:

    Article Title: Silencing KRAS Overexpression in Cadmium-Transformed Prostate Epithelial Cells Mitigates Malignant Phenotype
    Article Snippet: Keratinocyte-serum free medium (K-SFM), bovine pituitary extract (BPE), epidermal growth factor (EGF), and 100X antibiotic-antimycotic mixture were purchased from Life Technologies, Inc. (Grand Island, NY). .. GIPZ lentiviral KRAS shRNAmir particles (catalog no. VGH5523, clone ID: V3LHS_314009), and nonsilencing negative control shRNA (catalog no. RHS4348) were purchased from Thermo Fisher Scientific (Lafayette, CO). .. Puromycin was purchased from Cellgro (Manassas, VA).



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    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based <t>shRNA</t> silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of <t>normalized</t> <t>shCTRL</t> samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
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    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based <t>shRNA</t> silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of <t>normalized</t> <t>shCTRL</t> samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
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    Image Search Results


    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Journal: Cancers

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells

    doi: 10.3390/cancers13123055

    Figure Lengend Snippet: Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Article Snippet: Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific.

    Techniques: Western Blot, shRNA, MANN-WHITNEY, Marker