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transcription factor staining buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec transcription factor staining buffer
    Flow cytometry. A typical example of FACS based expression analysis for <t>transcription</t> factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed
    Transcription Factor Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/set/Transcription+Factor+Staining+Buffer+Set/pmc09209346-166-8-14
    Average 97 stars, based on 53 article reviews
    transcription factor staining buffer - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "The Treasury of Wharton's Jelly"

    Article Title: The Treasury of Wharton's Jelly

    Journal: Stem Cell Reviews and Reports

    doi: 10.1007/s12015-021-10217-8

    Flow cytometry. A typical example of FACS based expression analysis for transcription factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed
    Figure Legend Snippet: Flow cytometry. A typical example of FACS based expression analysis for transcription factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed

    Techniques Used: Flow Cytometry, Expressing, Fluorescence

    Expression of pluripotency associated marker proteins examined by Immunofluorescence staining. VSELs were separated from UC-MSCs mass population, the tiny cells are between 5 and 7 μm and surrounded with dark spots ( a ). VSEL suspension cells were stained extracellular with anti-bodies against SSEA-4, CD184, and CD133 followed by nuclear staining of transcription factors Nanog, Oct-4, and Sox-2. Expression of each marker was detected. The nuclei were stained with HOECHST. Weak background Fluorescence signals of isotype controls were subtracted. The scale bars represented 50 μm ( b ). The magnification for SSEA-4/Sox-2 and CD133/Oct-4 was 20-fold and for CD184/Nanog 10-fold
    Figure Legend Snippet: Expression of pluripotency associated marker proteins examined by Immunofluorescence staining. VSELs were separated from UC-MSCs mass population, the tiny cells are between 5 and 7 μm and surrounded with dark spots ( a ). VSEL suspension cells were stained extracellular with anti-bodies against SSEA-4, CD184, and CD133 followed by nuclear staining of transcription factors Nanog, Oct-4, and Sox-2. Expression of each marker was detected. The nuclei were stained with HOECHST. Weak background Fluorescence signals of isotype controls were subtracted. The scale bars represented 50 μm ( b ). The magnification for SSEA-4/Sox-2 and CD133/Oct-4 was 20-fold and for CD184/Nanog 10-fold

    Techniques Used: Expressing, Marker, Immunofluorescence, Staining, Suspension, Fluorescence

    Related Articles

    Staining:

    Article Title: Patient-Derived Immortalized Limbal Epithelial Cells as In Vitro Models of Congenital Aniridia.
    Article Snippet: Cells from eight independent passages (Supplementary Table S2) were harvested using Trypsin-EDTA solution (Cat. No. T3924, Merck KGaA, Darmstadt, Germany). .. With the exception of ABCG2, all antibodies targeted intracellular proteins; therefore, cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set (FixPerm; Cat. No. 130-122-981, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. ..

    Article Title: Generation of two LGMDR4 patients derived induced pluripotent stem cell line carrying the SGCB frameshift mutation (p.Gly129_Arg130insGlnTer).
    Article Snippet: Dissociated iPSC, P6, with accutase (BD Biosciences) were first stained with surface antibodies against SSEA4, TRA-1–60 and TRA-1–81 (Table 2) in BD PharmingenTM Stain Buffer (FBS) (BD Biosciences) at 4◦C for 10 min. .. The Transcription Factor Staining Buffer Set (Miltenyi Biotec) was used for fixation, permeabilization, and staining with intracellular antibodies against SOX2, NANOG and OCT4 (Table 2), according to the manufacturer’s instructions. .. Samples were analysed with the BD FACSMelodyTM Cell Sorter (BD Biosciences) and data were quantified with the FlowJO software. (FlowJo LLC).

    Article Title: Patient-Derived Immortalized Limbal Epithelial Cells as In Vitro Models of Congenital Aniridia
    Article Snippet: Cells from eight independent passages ( ) were harvested using Trypsin-EDTA solution (Cat. No. T3924, Merck KGaA, Darmstadt, Germany). .. With the exception of ABCG2, all antibodies targeted intracellular proteins; therefore, cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set (Fix-Perm; Cat. No. 130-122-981, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. ..

    Article Title: Competing gene regulatory networks drive naive and memory B cell differentiation.
    Article Snippet: On day 6, 5 × 105 cells were collected, washed in PBS, and stained with Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 10 min at 4 °C. .. The cells were washed with staining buffer and surface-stained for 30 min with anti-human CD20 Viobright B515 (Miltenyi), followed by intracellular staining with anti-human/mouse IRF4 eFluor 450 (Thermo Fisher Scientific) and anti-human PU.1 (SPI1) PE (BioLegend) monoclonal antibodies using the True-Nuclear Transcription Factor Buffer set (BioLegend) as per the manufacturer’s instructions. ..

    Article Title: miR-138-5p overexpression inhibits limbal epithelial cell proliferation and induces cell cycle arrest, in vitro.
    Article Snippet: A CytoFLEX flow cytometer (Beckman Coulter, CA, USA) was used to assess protein expression in pLECs transfected with either miR-138-5p or control (CTRL) mimics. .. After 48 and 72 h transfection, cells were detached using Trypsin-EDTA solution (Cat. No. T3924; Merck KGaA, Darmstadt, Germany), and the resulting pellets were fixed and permeabilized with the Transcription Factor Staining Buffer Set (Fix-Perm; Cat. No. 130-122-981; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany). ..

    Article Title: Disruption of macrophage migration inhibitory factor signaling induces major tumor-associated macrophage phenotypes in human M2 macrophages
    Article Snippet: After washing, cells were incubated with antibodies for 30 min at 4 °C in the dark in PBS (Lonza, Basel, Switzerland) containing 0.5% FBS (HyClone) and 50 μg·ml −1 human IgG (Octagam; Octapharma, Lachen, Switzerland) to prevent non-specific Fc receptor binding. .. Intracellular antigens were stained using the Transcription Factor Staining Buffer Set (130–122–981, Miltenyi Biotec) according to the manufacturer's protocol. .. Flow cytometric data were acquired on a FACSCanto II flow cytometer and analyzed using FACS Diva v6.1.2 (RRID:SCR_001456) and FlowJo v7.2.5 software (RRID:SCR_008520) (BD Biosciences).

    Bioprocessing:

    Article Title: Competing gene regulatory networks drive naive and memory B cell differentiation.
    Article Snippet: On day 6, 5 × 105 cells were collected, washed in PBS, and stained with Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) for 10 min at 4 °C. .. The cells were washed with staining buffer and surface-stained for 30 min with anti-human CD20 Viobright B515 (Miltenyi), followed by intracellular staining with anti-human/mouse IRF4 eFluor 450 (Thermo Fisher Scientific) and anti-human PU.1 (SPI1) PE (BioLegend) monoclonal antibodies using the True-Nuclear Transcription Factor Buffer set (BioLegend) as per the manufacturer’s instructions. ..

    Transfection:

    Article Title: miR-138-5p overexpression inhibits limbal epithelial cell proliferation and induces cell cycle arrest, in vitro.
    Article Snippet: A CytoFLEX flow cytometer (Beckman Coulter, CA, USA) was used to assess protein expression in pLECs transfected with either miR-138-5p or control (CTRL) mimics. .. After 48 and 72 h transfection, cells were detached using Trypsin-EDTA solution (Cat. No. T3924; Merck KGaA, Darmstadt, Germany), and the resulting pellets were fixed and permeabilized with the Transcription Factor Staining Buffer Set (Fix-Perm; Cat. No. 130-122-981; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany). ..



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    Image Search Results


    Full fabrication and application schematic diagram of GelMA-VEGF/ECM-PCSK9 composite hydrogel and the related signaling pathway of PCSK9 that promotes BMSC osteogenic differentiation.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Full fabrication and application schematic diagram of GelMA-VEGF/ECM-PCSK9 composite hydrogel and the related signaling pathway of PCSK9 that promotes BMSC osteogenic differentiation.

    Article Snippet: VEGF, ELISA kit for VEGF and PCSK9 were purchased from Boster company (Wuhan, China).

    Techniques:

    Construction and characterization of GelMA-VEGF/ECM-PCSK9 composite hydrogel. A Schematic diagram showing the process of composite hydrogel construction; B) Photographs of GelMA-VEGF hydrogel and GelMA-VEGF/ECM-PCSK9 hydrogel formation after UV light respectively; C i) Electron microscopic image of pure GelMA hydrogel, with a scale of 100 μm; ii) Enlarged electron microscopic image of GelMA hydrogel, with a scale of 50 μm; D) i The electron microscope image of the combination of GelMA hydrogel and ECM, with a scale of 100 μm; ii Electron microscope magnified image of GelMA hydrogel combined with ECM, with a scale of 50 μm; E) The infrared spectrum (FITR) diagram of the acellular ECM, GelMA hydrogel and GelMA/ECM composite hydrogel contains common basic energy groups; F) Load rate of PCSK9 in ECM; G) Release rate of VEGF loaded with GelMA hydrogel and GelMA/ECM composite hydrogel respectively; H) Release rate of PCSK9 loaded with ECM and GelMA/ECM composite hydrogel respectively; I) Release rate of VEGF and PCSK9 loaded in GelMA and GelMA/ECM on different time points respectively; J) Release rate of VEGF and PCSK9 respectively when loaded in GelMA/ECM; K) The swelling rate of GelMA gel and GelMA/ECM composite gel dissolved in PBS (n = 6); L) Degradation rate of GelMA hydrogel and GelMA/ECM composite gel in vitro (n = 6).∗means that compared with the control group, p < 0.05; ∗means that compared with the control group, p < 0.01; ∗∗∗means that compared with the control group, p < 0.001.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Construction and characterization of GelMA-VEGF/ECM-PCSK9 composite hydrogel. A Schematic diagram showing the process of composite hydrogel construction; B) Photographs of GelMA-VEGF hydrogel and GelMA-VEGF/ECM-PCSK9 hydrogel formation after UV light respectively; C i) Electron microscopic image of pure GelMA hydrogel, with a scale of 100 μm; ii) Enlarged electron microscopic image of GelMA hydrogel, with a scale of 50 μm; D) i The electron microscope image of the combination of GelMA hydrogel and ECM, with a scale of 100 μm; ii Electron microscope magnified image of GelMA hydrogel combined with ECM, with a scale of 50 μm; E) The infrared spectrum (FITR) diagram of the acellular ECM, GelMA hydrogel and GelMA/ECM composite hydrogel contains common basic energy groups; F) Load rate of PCSK9 in ECM; G) Release rate of VEGF loaded with GelMA hydrogel and GelMA/ECM composite hydrogel respectively; H) Release rate of PCSK9 loaded with ECM and GelMA/ECM composite hydrogel respectively; I) Release rate of VEGF and PCSK9 loaded in GelMA and GelMA/ECM on different time points respectively; J) Release rate of VEGF and PCSK9 respectively when loaded in GelMA/ECM; K) The swelling rate of GelMA gel and GelMA/ECM composite gel dissolved in PBS (n = 6); L) Degradation rate of GelMA hydrogel and GelMA/ECM composite gel in vitro (n = 6).∗means that compared with the control group, p < 0.05; ∗means that compared with the control group, p < 0.01; ∗∗∗means that compared with the control group, p < 0.001.

    Article Snippet: VEGF, ELISA kit for VEGF and PCSK9 were purchased from Boster company (Wuhan, China).

    Techniques: Microscopy, In Vitro, Control

    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Article Snippet: VEGF, ELISA kit for VEGF and PCSK9 were purchased from Boster company (Wuhan, China).

    Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control

    The effect of different composite hydrogel on the osteogenic differentiation of BMMSC in vitro. Cultivate BMMSC for osteogenic differentiation in osteogenic medium with GelMA, GelMA-VEGF, GelMA-VEGF/ECM, ECM-PCSK9, and GelMA-VEGF/ECM-PCSK9 for 7 days respectively. A,B) The cell nucleus was stained with DAPI (blue), RUNX2 was stained with RUNX2 antibody (green), and COL1A1 was stained with COL1A1 antibody (red), with a scale bar of 200 μm. C,D) The quantitative analysis results of COL1A1 and RUNX2 immunofluorescence images; E,F) Quantitative analysis of ALP staining and ARS staining for BMMSC co-culture with different kinds of hydrogels; G) ALP staining result for BMMSC co-culture with different kinds of hydrogels for 7days, scale bar = 200 μm; F) ARS staining result for BMMSC co-culture with different kinds of hydrogels for 14days, scale bar = 200 μm; I, J) After 7 and 14 days of co-culture with different combinations of composite hydrogels and BMMSC for osteogenesis and differentiation, the PCR experiment results of osteogenesis related indicators suggest that compared with the control group. G = simple GelMA hydrogel group, GV=GelMA hydrogels + VEGF protein group, GV/E = GelMA + VEGF/ECM group, EP = ECM + PCSK9 protein group, GVEP=GelMA + VEGF/ECM + PCSK9 protein group, the significant differences between the groups are expressed as ∗ p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ns means there is no significant difference between the groups.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: The effect of different composite hydrogel on the osteogenic differentiation of BMMSC in vitro. Cultivate BMMSC for osteogenic differentiation in osteogenic medium with GelMA, GelMA-VEGF, GelMA-VEGF/ECM, ECM-PCSK9, and GelMA-VEGF/ECM-PCSK9 for 7 days respectively. A,B) The cell nucleus was stained with DAPI (blue), RUNX2 was stained with RUNX2 antibody (green), and COL1A1 was stained with COL1A1 antibody (red), with a scale bar of 200 μm. C,D) The quantitative analysis results of COL1A1 and RUNX2 immunofluorescence images; E,F) Quantitative analysis of ALP staining and ARS staining for BMMSC co-culture with different kinds of hydrogels; G) ALP staining result for BMMSC co-culture with different kinds of hydrogels for 7days, scale bar = 200 μm; F) ARS staining result for BMMSC co-culture with different kinds of hydrogels for 14days, scale bar = 200 μm; I, J) After 7 and 14 days of co-culture with different combinations of composite hydrogels and BMMSC for osteogenesis and differentiation, the PCR experiment results of osteogenesis related indicators suggest that compared with the control group. G = simple GelMA hydrogel group, GV=GelMA hydrogels + VEGF protein group, GV/E = GelMA + VEGF/ECM group, EP = ECM + PCSK9 protein group, GVEP=GelMA + VEGF/ECM + PCSK9 protein group, the significant differences between the groups are expressed as ∗ p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ns means there is no significant difference between the groups.

    Article Snippet: VEGF, ELISA kit for VEGF and PCSK9 were purchased from Boster company (Wuhan, China).

    Techniques: In Vitro, Staining, Immunofluorescence, Co-Culture Assay, Control

    After adding different concentrations of PCSK9 to BMMSC for osteogenic induction, western blotting (WB) experiment was performed to evaluate the expression of phosphorylated proteins and total proteins among different osteogenic differentiation relevant signaling pathways. A) WB images of different signaling pathways that related to osteogenic differentiation after adding different concentrations of PCSK9; B-D) Quantitative analysis results of phosphorylated protein and total protein. Compared with the control group, ∗ means p < 0.05, ∗∗ means p < 0.01.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: After adding different concentrations of PCSK9 to BMMSC for osteogenic induction, western blotting (WB) experiment was performed to evaluate the expression of phosphorylated proteins and total proteins among different osteogenic differentiation relevant signaling pathways. A) WB images of different signaling pathways that related to osteogenic differentiation after adding different concentrations of PCSK9; B-D) Quantitative analysis results of phosphorylated protein and total protein. Compared with the control group, ∗ means p < 0.05, ∗∗ means p < 0.01.

    Article Snippet: VEGF, ELISA kit for VEGF and PCSK9 were purchased from Boster company (Wuhan, China).

    Techniques: Western Blot, Expressing, Protein-Protein interactions, Control

    Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

    Journal: Bioactive Materials

    Article Title: Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

    doi: 10.1016/j.bioactmat.2026.03.025

    Figure Lengend Snippet: Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

    Article Snippet: IL-6 and IL-10-specific antibodies were purchased from Bosterbio (Wuhan, China).

    Techniques: Immunofluorescence, Staining, Fluorescence