transcription factor staining buffer (Miltenyi Biotec)
Structured Review

Transcription Factor Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/set/Transcription+Factor+Staining+Buffer+Set/pmc09209346-166-8-14
Average 97 stars, based on 53 article reviews
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1) Product Images from "The Treasury of Wharton's Jelly"
Article Title: The Treasury of Wharton's Jelly
Journal: Stem Cell Reviews and Reports
doi: 10.1007/s12015-021-10217-8
Figure Legend Snippet: Flow cytometry. A typical example of FACS based expression analysis for transcription factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed
Techniques Used: Flow Cytometry, Expressing, Fluorescence
Figure Legend Snippet: Expression of pluripotency associated marker proteins examined by Immunofluorescence staining. VSELs were separated from UC-MSCs mass population, the tiny cells are between 5 and 7 μm and surrounded with dark spots ( a ). VSEL suspension cells were stained extracellular with anti-bodies against SSEA-4, CD184, and CD133 followed by nuclear staining of transcription factors Nanog, Oct-4, and Sox-2. Expression of each marker was detected. The nuclei were stained with HOECHST. Weak background Fluorescence signals of isotype controls were subtracted. The scale bars represented 50 μm ( b ). The magnification for SSEA-4/Sox-2 and CD133/Oct-4 was 20-fold and for CD184/Nanog 10-fold
Techniques Used: Expressing, Marker, Immunofluorescence, Staining, Suspension, Fluorescence
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