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sequenom® massarray assay design 3.1 software  (Sequenom)

 
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    Sequenom sequenom® massarray assay design 3.1 software
    Sequenom® Massarray Assay Design 3.1 Software, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequenome+massarray+designer+software/sequenom+spectrodesigner+software/pmc03357346-102-16-15
    Average 90 stars, based on 1 article reviews
    sequenom® massarray assay design 3.1 software - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: The NEI/NCBI dbGAP database: Genotypes and haplotypes that may specifically predispose to risk of neovascular age-related macular degeneration
    Article Snippet: For both the Sequenom iPLEX system technology and direct sequencing protocols, leukocyte DNA was either purified by using standard phenol-chloroform or DNAzol (Invitrogen Corporation, Carlsbad, California) extraction protocols. .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 bp of flanking sequence on either side of the SNP. .. Briefly, 10 ng genomic DNA was amplified in a 5 ul reaction containing 1 × HotStar Taq PCR buffer (Qiagen), 1.625 mM MgCl 2 , 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Influence of ROBO1 and RORA on Risk of Age-Related Macular Degeneration Reveals Genetically Distinct Phenotypes in Disease Pathophysiology
    Article Snippet: These SNPs were genotyped using a combination of Sequenom and TaqMan. .. For the SNPs genotyped via Sequenom, multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) (Sequenom, San Diego, CA) by inputting sequence containing the SNP site and 100 base pair (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 uL reaction containing 1X HotStar Taq PCR buffer (Qiagen, Valencia, CA), 1.625 mM MgCl2, 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Systems biology-based analysis implicates a novel role for vitamin D metabolism in the pathogenesis of age-related macular degeneration
    Article Snippet: In total, 59 SNPs were initially screened by Sequenom (San Diego, CA, USA) in the extremely discordant sibling pair discovery cohort (135 sibling pairs, 270 individuals): CYP27A1 , n = 4; VDR , n = 33; CYP27B1 , n = 2; CYP24A1 , n = 20 (Table S2 (Table )). .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 base pairs (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 μl reaction containing 1 × HotStar Taq PCR buffer (Qiagen, Valencia, CA, USA), 1.625 mM MgCl 2 , 500 μl each dNTP, 100 nM each PCR primer and 0.5 U HotStar Taq (Qiagen).

    Polymerase Chain Reaction:

    Article Title: The NEI/NCBI dbGAP database: Genotypes and haplotypes that may specifically predispose to risk of neovascular age-related macular degeneration
    Article Snippet: For both the Sequenom iPLEX system technology and direct sequencing protocols, leukocyte DNA was either purified by using standard phenol-chloroform or DNAzol (Invitrogen Corporation, Carlsbad, California) extraction protocols. .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 bp of flanking sequence on either side of the SNP. .. Briefly, 10 ng genomic DNA was amplified in a 5 ul reaction containing 1 × HotStar Taq PCR buffer (Qiagen), 1.625 mM MgCl 2 , 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Influence of ROBO1 and RORA on Risk of Age-Related Macular Degeneration Reveals Genetically Distinct Phenotypes in Disease Pathophysiology
    Article Snippet: These SNPs were genotyped using a combination of Sequenom and TaqMan. .. For the SNPs genotyped via Sequenom, multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) (Sequenom, San Diego, CA) by inputting sequence containing the SNP site and 100 base pair (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 uL reaction containing 1X HotStar Taq PCR buffer (Qiagen, Valencia, CA), 1.625 mM MgCl2, 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Systems biology-based analysis implicates a novel role for vitamin D metabolism in the pathogenesis of age-related macular degeneration
    Article Snippet: In total, 59 SNPs were initially screened by Sequenom (San Diego, CA, USA) in the extremely discordant sibling pair discovery cohort (135 sibling pairs, 270 individuals): CYP27A1 , n = 4; VDR , n = 33; CYP27B1 , n = 2; CYP24A1 , n = 20 (Table S2 (Table )). .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 base pairs (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 μl reaction containing 1 × HotStar Taq PCR buffer (Qiagen, Valencia, CA, USA), 1.625 mM MgCl 2 , 500 μl each dNTP, 100 nM each PCR primer and 0.5 U HotStar Taq (Qiagen).

    Article Title: Genomewide Analysis of Inherited Variation Associated with Phosphorylation of PI3K/AKT/mTOR Signaling Proteins
    Article Snippet: SNP genotyping was performed using the Sequenom platform and DNA that had been extracted from cell lines from the 14 CEPH families. .. PCR and extension primers were designed using Sequenom AssayDesign software ( ). .. PCR reactions, shrimp alkaline phosphatase digestion, and extension reactions were performed according to Sequenom's standard protocol with one exception: a linear adjustment to the PCR primer concentrations was made in an attempt to standardize mass spectrometer peak heights.

    Software:

    Article Title: The NEI/NCBI dbGAP database: Genotypes and haplotypes that may specifically predispose to risk of neovascular age-related macular degeneration
    Article Snippet: For both the Sequenom iPLEX system technology and direct sequencing protocols, leukocyte DNA was either purified by using standard phenol-chloroform or DNAzol (Invitrogen Corporation, Carlsbad, California) extraction protocols. .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 bp of flanking sequence on either side of the SNP. .. Briefly, 10 ng genomic DNA was amplified in a 5 ul reaction containing 1 × HotStar Taq PCR buffer (Qiagen), 1.625 mM MgCl 2 , 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Influence of ROBO1 and RORA on Risk of Age-Related Macular Degeneration Reveals Genetically Distinct Phenotypes in Disease Pathophysiology
    Article Snippet: These SNPs were genotyped using a combination of Sequenom and TaqMan. .. For the SNPs genotyped via Sequenom, multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) (Sequenom, San Diego, CA) by inputting sequence containing the SNP site and 100 base pair (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 uL reaction containing 1X HotStar Taq PCR buffer (Qiagen, Valencia, CA), 1.625 mM MgCl2, 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Systems biology-based analysis implicates a novel role for vitamin D metabolism in the pathogenesis of age-related macular degeneration
    Article Snippet: In total, 59 SNPs were initially screened by Sequenom (San Diego, CA, USA) in the extremely discordant sibling pair discovery cohort (135 sibling pairs, 270 individuals): CYP27A1 , n = 4; VDR , n = 33; CYP27B1 , n = 2; CYP24A1 , n = 20 (Table S2 (Table )). .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 base pairs (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 μl reaction containing 1 × HotStar Taq PCR buffer (Qiagen, Valencia, CA, USA), 1.625 mM MgCl 2 , 500 μl each dNTP, 100 nM each PCR primer and 0.5 U HotStar Taq (Qiagen).

    Article Title: KIAA1462 , A Coronary Artery Disease Associated Gene, Is a Candidate Gene for Late Onset Alzheimer Disease in APOE Carriers
    Article Snippet: A total of 384 SNPs on chr10 were genotyped with the use of the midthroughput Sequenom genotyping platform, based on a single-base primer extension reaction coupled with mass spectrometry. .. The assays were designed using Sequenom SpectroDESIGNER software. .. Genomic DNA (5 ng) was amplified following the manufacturer recommendations (Sequenom).

    Article Title: Genomewide Analysis of Inherited Variation Associated with Phosphorylation of PI3K/AKT/mTOR Signaling Proteins
    Article Snippet: SNP genotyping was performed using the Sequenom platform and DNA that had been extracted from cell lines from the 14 CEPH families. .. PCR and extension primers were designed using Sequenom AssayDesign software ( ). .. PCR reactions, shrimp alkaline phosphatase digestion, and extension reactions were performed according to Sequenom's standard protocol with one exception: a linear adjustment to the PCR primer concentrations was made in an attempt to standardize mass spectrometer peak heights.

    Sequencing:

    Article Title: The NEI/NCBI dbGAP database: Genotypes and haplotypes that may specifically predispose to risk of neovascular age-related macular degeneration
    Article Snippet: For both the Sequenom iPLEX system technology and direct sequencing protocols, leukocyte DNA was either purified by using standard phenol-chloroform or DNAzol (Invitrogen Corporation, Carlsbad, California) extraction protocols. .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 bp of flanking sequence on either side of the SNP. .. Briefly, 10 ng genomic DNA was amplified in a 5 ul reaction containing 1 × HotStar Taq PCR buffer (Qiagen), 1.625 mM MgCl 2 , 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Influence of ROBO1 and RORA on Risk of Age-Related Macular Degeneration Reveals Genetically Distinct Phenotypes in Disease Pathophysiology
    Article Snippet: These SNPs were genotyped using a combination of Sequenom and TaqMan. .. For the SNPs genotyped via Sequenom, multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) (Sequenom, San Diego, CA) by inputting sequence containing the SNP site and 100 base pair (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 uL reaction containing 1X HotStar Taq PCR buffer (Qiagen, Valencia, CA), 1.625 mM MgCl2, 500 uM each dNTP, 100 nM each PCR primer, 0.5 U HotStar Taq (Qiagen).

    Article Title: Systems biology-based analysis implicates a novel role for vitamin D metabolism in the pathogenesis of age-related macular degeneration
    Article Snippet: In total, 59 SNPs were initially screened by Sequenom (San Diego, CA, USA) in the extremely discordant sibling pair discovery cohort (135 sibling pairs, 270 individuals): CYP27A1 , n = 4; VDR , n = 33; CYP27B1 , n = 2; CYP24A1 , n = 20 (Table S2 (Table )). .. Multiplex PCR assays were designed using Sequenom SpectroDESIGNER software (version 3.0.0.3) by inputting sequence containing the SNP site and 100 base pairs (bp) of flanking sequence on either side of the SNP. .. Briefly, 10 ng of genomic DNA was amplified in a 5 μl reaction containing 1 × HotStar Taq PCR buffer (Qiagen, Valencia, CA, USA), 1.625 mM MgCl 2 , 500 μl each dNTP, 100 nM each PCR primer and 0.5 U HotStar Taq (Qiagen).

    other:

    Article Title: Impact of OXTR Polymorphisms on Subjective Well-Being: The Intermediary Role of Attributional Style
    Article Snippet: My-Sequenom online software Assay Design Suite v2.0 was applied to design probes and primers ( ).

    Article Title: Association and functional study between ADIPOQ and metabolic syndrome in elderly Chinese Han population
    Article Snippet: The primers and probes for PCR were designed using the online Assay Design Suite v2.0 Sequenom software.

    Article Title: Personality traits as mediators in the association between SIRT1 rs12415800 polymorphism and depressive symptoms among Chinese college students
    Article Snippet: Probes and primers were designed using the My-Sequenom online software Assay Design Suite v2.0.



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