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Microsynth ag sequencing service
Sequencing Service, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Enzymatic absorption promoters for non-invasive peptide delivery.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Treatment of avian malaria in captive African penguins ( Spheniscus demersus ) by the combination of atovaquone and proguanil hydrochloride
Article Snippet: Amplicons were purified using the PureLinkTM Quick Gel Extraction & PCR Purification Combo Kit (ThermoFisher Scientific, Milan, Italy) and sequenced at the Microsynth Seqlab facilities (Göttingen, Germany) using the primers for the nPCR 2 nd step HAEMN-F (5”-ATGGTGCTTTMGATATATGCATG-3‘) and HAEMN-R2 (5’- GCATTATCTGGATGTGATAATGGT-3”) [ ].

Polymerase Chain Reaction:

Article Title: Q1020R in the spike proteins of MERS-CoV from Arabian camels and recent human cases confers resistance against soluble human DPP4
Article Snippet: .. The integrity of all PCR-amplified sequences was verified by using a commercial sequencing service (Microsynth SeqLab). .. The following cell lines were used in the present study: Vero cells (African green monkey kidney, female; ATCC no. CRL-1586, RRID: CVCL_0603, kindly provided by Andrea Maisner), Huh-7 cells (human liver, male; JCRB no. JCRB0403; RRID: CVCL 0336, kindly provided by Thomas Pietschmann), BHK-21 (Syrian hamster, kidney cells; ATCC no. CCL-10; RRID: CVCL_10929, kindly provided by Georg Herrler), and 293T cells (human kidney, female; DSMZ no. ACC-635; RRID: CVCL_0063) were cultured in Dulbecco’s modified Eagle medium (DMEM, PAN-Biotech) supplemented with 10% fetal bovine serum (FBS, Biochrom), 1% 100 U/ml penicillin and 0.1 mg/ml streptomycin (pen/strep) (PAN-Biotech).

Article Title:
Article Snippet: .. A commercial sequencing service was used to confirm all PCR-amplified sequences (Microsynth SeqLab). ..

Article Title: Autonomous bioluminescence emission from transgenic mice.
Article Snippet: .. To verify the DNA sequence of the complete expression cassette, overlapping fragments ~1 kb in length were generated by PCR amplification from genomic DNA using the primer pairs shown in table S3, purified from agarose gels, and sequenced using a standard sequencing service (Microsynth Seqlab GmbH). .. Bioluminescence imaging of living and dead mice was performed with an IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Sequencing:

Article Title: Q1020R in the spike proteins of MERS-CoV from Arabian camels and recent human cases confers resistance against soluble human DPP4
Article Snippet: .. The integrity of all PCR-amplified sequences was verified by using a commercial sequencing service (Microsynth SeqLab). .. The following cell lines were used in the present study: Vero cells (African green monkey kidney, female; ATCC no. CRL-1586, RRID: CVCL_0603, kindly provided by Andrea Maisner), Huh-7 cells (human liver, male; JCRB no. JCRB0403; RRID: CVCL 0336, kindly provided by Thomas Pietschmann), BHK-21 (Syrian hamster, kidney cells; ATCC no. CCL-10; RRID: CVCL_10929, kindly provided by Georg Herrler), and 293T cells (human kidney, female; DSMZ no. ACC-635; RRID: CVCL_0063) were cultured in Dulbecco’s modified Eagle medium (DMEM, PAN-Biotech) supplemented with 10% fetal bovine serum (FBS, Biochrom), 1% 100 U/ml penicillin and 0.1 mg/ml streptomycin (pen/strep) (PAN-Biotech).

Article Title:
Article Snippet: .. A commercial sequencing service was used to confirm all PCR-amplified sequences (Microsynth SeqLab). ..

Article Title: Autonomous bioluminescence emission from transgenic mice.
Article Snippet: .. To verify the DNA sequence of the complete expression cassette, overlapping fragments ~1 kb in length were generated by PCR amplification from genomic DNA using the primer pairs shown in table S3, purified from agarose gels, and sequenced using a standard sequencing service (Microsynth Seqlab GmbH). .. Bioluminescence imaging of living and dead mice was performed with an IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Article Title: Efficient Design of Affilin ® Protein Binders for HER3.
Article Snippet: Genes for Affilin® proteins Comp-1–Comp-12 & Exp-MatComp-1–Exp-MatComp-4 containing more than one amino acid mutation were synthesized as string fragments (GeneArt; Regensburg, Germany) and cloned into pNP-002 as described above. .. Sequence identities were confirmed by external sequencing services (Microsynth AG; Balgach, Switzerland). ..

Expressing:

Article Title: Autonomous bioluminescence emission from transgenic mice.
Article Snippet: .. To verify the DNA sequence of the complete expression cassette, overlapping fragments ~1 kb in length were generated by PCR amplification from genomic DNA using the primer pairs shown in table S3, purified from agarose gels, and sequenced using a standard sequencing service (Microsynth Seqlab GmbH). .. Bioluminescence imaging of living and dead mice was performed with an IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Generated:

Article Title: Autonomous bioluminescence emission from transgenic mice.
Article Snippet: .. To verify the DNA sequence of the complete expression cassette, overlapping fragments ~1 kb in length were generated by PCR amplification from genomic DNA using the primer pairs shown in table S3, purified from agarose gels, and sequenced using a standard sequencing service (Microsynth Seqlab GmbH). .. Bioluminescence imaging of living and dead mice was performed with an IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Amplification:

Article Title: Autonomous bioluminescence emission from transgenic mice.
Article Snippet: .. To verify the DNA sequence of the complete expression cassette, overlapping fragments ~1 kb in length were generated by PCR amplification from genomic DNA using the primer pairs shown in table S3, purified from agarose gels, and sequenced using a standard sequencing service (Microsynth Seqlab GmbH). .. Bioluminescence imaging of living and dead mice was performed with an IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Purification:

Article Title: Autonomous bioluminescence emission from transgenic mice.
Article Snippet: .. To verify the DNA sequence of the complete expression cassette, overlapping fragments ~1 kb in length were generated by PCR amplification from genomic DNA using the primer pairs shown in table S3, purified from agarose gels, and sequenced using a standard sequencing service (Microsynth Seqlab GmbH). .. Bioluminescence imaging of living and dead mice was performed with an IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Saline:

Article Title: Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease
Article Snippet: Next, cells were stained for zombie violet fixable viability dye (Biolegend) 30 min at 4°C, and permeabilized for 20 min at 4°C with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). .. Next, cells were washed with 2x saline-sodium citrate (SSC) buffer (20x SSC buffer, Roth, 1054.1) and stained with a 16Ss rRNA FISH probe EUB338 (5’-GCTGCCTCCCGTA-GGAGT-3), Cy5 (Microsynth) or an antisense probe (5’-ACTCCTACGG-GAGGCAGC-3’), Cy5 (Microsynth) in hybridization buffer (25% formamide Huberlab, A2156.0500), 10% dextran sulfate (ThermoFisher, 15415089), 0.5% bovine serum albumin (BSA) (Pan-Biotec, PANP06-1391500), 2x SSC buffer) and incubated overnight at 37°C in 5% CO2 atmosphere. ..

Staining:

Article Title: Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease
Article Snippet: Next, cells were stained for zombie violet fixable viability dye (Biolegend) 30 min at 4°C, and permeabilized for 20 min at 4°C with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). .. Next, cells were washed with 2x saline-sodium citrate (SSC) buffer (20x SSC buffer, Roth, 1054.1) and stained with a 16Ss rRNA FISH probe EUB338 (5’-GCTGCCTCCCGTA-GGAGT-3), Cy5 (Microsynth) or an antisense probe (5’-ACTCCTACGG-GAGGCAGC-3’), Cy5 (Microsynth) in hybridization buffer (25% formamide Huberlab, A2156.0500), 10% dextran sulfate (ThermoFisher, 15415089), 0.5% bovine serum albumin (BSA) (Pan-Biotec, PANP06-1391500), 2x SSC buffer) and incubated overnight at 37°C in 5% CO2 atmosphere. ..

Fluorescence In Situ Hybridization:

Article Title: Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease
Article Snippet: Next, cells were stained for zombie violet fixable viability dye (Biolegend) 30 min at 4°C, and permeabilized for 20 min at 4°C with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). .. Next, cells were washed with 2x saline-sodium citrate (SSC) buffer (20x SSC buffer, Roth, 1054.1) and stained with a 16Ss rRNA FISH probe EUB338 (5’-GCTGCCTCCCGTA-GGAGT-3), Cy5 (Microsynth) or an antisense probe (5’-ACTCCTACGG-GAGGCAGC-3’), Cy5 (Microsynth) in hybridization buffer (25% formamide Huberlab, A2156.0500), 10% dextran sulfate (ThermoFisher, 15415089), 0.5% bovine serum albumin (BSA) (Pan-Biotec, PANP06-1391500), 2x SSC buffer) and incubated overnight at 37°C in 5% CO2 atmosphere. ..

Hybridization:

Article Title: Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease
Article Snippet: Next, cells were stained for zombie violet fixable viability dye (Biolegend) 30 min at 4°C, and permeabilized for 20 min at 4°C with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). .. Next, cells were washed with 2x saline-sodium citrate (SSC) buffer (20x SSC buffer, Roth, 1054.1) and stained with a 16Ss rRNA FISH probe EUB338 (5’-GCTGCCTCCCGTA-GGAGT-3), Cy5 (Microsynth) or an antisense probe (5’-ACTCCTACGG-GAGGCAGC-3’), Cy5 (Microsynth) in hybridization buffer (25% formamide Huberlab, A2156.0500), 10% dextran sulfate (ThermoFisher, 15415089), 0.5% bovine serum albumin (BSA) (Pan-Biotec, PANP06-1391500), 2x SSC buffer) and incubated overnight at 37°C in 5% CO2 atmosphere. ..

Incubation:

Article Title: Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease
Article Snippet: Next, cells were stained for zombie violet fixable viability dye (Biolegend) 30 min at 4°C, and permeabilized for 20 min at 4°C with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). .. Next, cells were washed with 2x saline-sodium citrate (SSC) buffer (20x SSC buffer, Roth, 1054.1) and stained with a 16Ss rRNA FISH probe EUB338 (5’-GCTGCCTCCCGTA-GGAGT-3), Cy5 (Microsynth) or an antisense probe (5’-ACTCCTACGG-GAGGCAGC-3’), Cy5 (Microsynth) in hybridization buffer (25% formamide Huberlab, A2156.0500), 10% dextran sulfate (ThermoFisher, 15415089), 0.5% bovine serum albumin (BSA) (Pan-Biotec, PANP06-1391500), 2x SSC buffer) and incubated overnight at 37°C in 5% CO2 atmosphere. ..



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Image Search Results


(A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific DNA inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.

Journal: bioRxiv

Article Title: Engineering Persistent and Rewritable Genetic Memory in Bacteria through Multiscale Plasmid Competition

doi: 10.64898/2026.09.18.752696

Figure Lengend Snippet: (A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific DNA inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.

Article Snippet: DNA sequencing used Plasmidsaurus DNA whole plasmid sequencing service.

Techniques: Expressing, Flow Cytometry, Analysis

(A) Corallimorpharian mucus collection. After initial salt water dripped off animals and plates, mucus was collected from adult Corallimorpharian animals by tilting over 1.5 mL centrifuge tubes and collecting the run-off. B,C) R. florida mucus exhibit consistently higher CFU counts than those that harbored seawater. Corallimorph mucus (A) and surrounding sea water (C) were cultured on Marine Agar plates and the number of colony forming units were quantified. ( D) Corallimorpharian mucus harbors morphologically diverse bacterial isolates. Colonies isolated from R. florida mucus exhibited substantial variation in pigmentation and morphology, including dark purple/black (A), translucent cream (B-D), white (E), and peach (F) morphotypes. Isolates also differed in colony architecture and growth characteristics. Among these, one isolate displayed an unusual agar-pitting phenotype (G), suggesting extracellular agar-degrading activity. ( E,F) R. florida were infected with A2 Vibrio . Clonal polyps from control treatment (E) and high bacterial exposure (F) were pooled in equal quantities for mRNA library construction and sequencing using MiSeq technology (Illumina).

Journal: bioRxiv

Article Title: Comparative genomics and microbiome profiling reveal a conserved Vibrio rich mucus microbiota of the Florida false coral ( Ricordea florida )

doi: 10.64898/2026.09.17.752451

Figure Lengend Snippet: (A) Corallimorpharian mucus collection. After initial salt water dripped off animals and plates, mucus was collected from adult Corallimorpharian animals by tilting over 1.5 mL centrifuge tubes and collecting the run-off. B,C) R. florida mucus exhibit consistently higher CFU counts than those that harbored seawater. Corallimorph mucus (A) and surrounding sea water (C) were cultured on Marine Agar plates and the number of colony forming units were quantified. ( D) Corallimorpharian mucus harbors morphologically diverse bacterial isolates. Colonies isolated from R. florida mucus exhibited substantial variation in pigmentation and morphology, including dark purple/black (A), translucent cream (B-D), white (E), and peach (F) morphotypes. Isolates also differed in colony architecture and growth characteristics. Among these, one isolate displayed an unusual agar-pitting phenotype (G), suggesting extracellular agar-degrading activity. ( E,F) R. florida were infected with A2 Vibrio . Clonal polyps from control treatment (E) and high bacterial exposure (F) were pooled in equal quantities for mRNA library construction and sequencing using MiSeq technology (Illumina).

Article Snippet: Some isolates were re-sequenced using the Plasmidsaurus Whole Genome Sequencing service (Oxford Nanopore, R10.4.1 long-reads and de novo genome assembly).

Techniques: Cell Culture, Isolation, Morphology, Cream, Activity Assay, Infection, Control, Sequencing

A. Relative abundance (%) of bacteria Genera collected from polyps from distinct tank systems reveals a dominance of Vibrio . Columns of each bar graph represent a polyp (A or B) and tank collected from is noted with the number. B. Polyps from the same tank show no difference in bacterial assemblage. A principal component analysis (PCA) reveals no differences in structure of bacterial communities between tank systems (12, 14, and 15 represented by blue, green and purple circles). X and Y axes show Principal Component 1 and Principal Component 2 that explain 56% and 22% of the total variance, respectively. (C). Shared and unique ASVs among corallimorph-associated bacterial communities. UpSet plot showing the overlap of amplicon sequence variants (ASVs) among Tanks 12, 14, and 15. An ASV was considered present within a tank only if it was detected in both animals sampled from that tank. Numbers indicate ASVs unique to individual tanks or shared among multiple tanks.

Journal: bioRxiv

Article Title: Comparative genomics and microbiome profiling reveal a conserved Vibrio rich mucus microbiota of the Florida false coral ( Ricordea florida )

doi: 10.64898/2026.09.17.752451

Figure Lengend Snippet: A. Relative abundance (%) of bacteria Genera collected from polyps from distinct tank systems reveals a dominance of Vibrio . Columns of each bar graph represent a polyp (A or B) and tank collected from is noted with the number. B. Polyps from the same tank show no difference in bacterial assemblage. A principal component analysis (PCA) reveals no differences in structure of bacterial communities between tank systems (12, 14, and 15 represented by blue, green and purple circles). X and Y axes show Principal Component 1 and Principal Component 2 that explain 56% and 22% of the total variance, respectively. (C). Shared and unique ASVs among corallimorph-associated bacterial communities. UpSet plot showing the overlap of amplicon sequence variants (ASVs) among Tanks 12, 14, and 15. An ASV was considered present within a tank only if it was detected in both animals sampled from that tank. Numbers indicate ASVs unique to individual tanks or shared among multiple tanks.

Article Snippet: Some isolates were re-sequenced using the Plasmidsaurus Whole Genome Sequencing service (Oxford Nanopore, R10.4.1 long-reads and de novo genome assembly).

Techniques: Bacteria, Analysis, Amplification, Sequencing

HeLa:Cas9 cells in 10cm plates were transfected with overexpression constructs for HPV16L2, encoding a C-terminal HA tag, and either an overexpression construct for PLCδ4 or PLCγ1, both containing the sequence for both C-terminal FLAG tag and c-Myc tag, or pCMV6, an empty vector control. Transfected cells were harvested 24h post-transfection, lysed in buffer containing 1% NP40 and 0.1% SDS, and then (cleared) lysates were incubated with magnetic beads coupled to anti-HA antibodies to pull down L2. Co-immunoprecipitation (CoIP) of PLCδ4 in bead elutions (“Elutes”) was determined using Western blot. Western blotting against the HA and c-Myc tags was used to confirm the presence of L2, PLCδ4, and PLCγ1 in “elutes” and “cleared lysates”. Blots are representative of three independent experiments ( N =3).

Journal: bioRxiv

Article Title: HPV16 Utilizes Phospholipase C(s) for its Genome Egress

doi: 10.64898/2026.09.03.749318

Figure Lengend Snippet: HeLa:Cas9 cells in 10cm plates were transfected with overexpression constructs for HPV16L2, encoding a C-terminal HA tag, and either an overexpression construct for PLCδ4 or PLCγ1, both containing the sequence for both C-terminal FLAG tag and c-Myc tag, or pCMV6, an empty vector control. Transfected cells were harvested 24h post-transfection, lysed in buffer containing 1% NP40 and 0.1% SDS, and then (cleared) lysates were incubated with magnetic beads coupled to anti-HA antibodies to pull down L2. Co-immunoprecipitation (CoIP) of PLCδ4 in bead elutions (“Elutes”) was determined using Western blot. Western blotting against the HA and c-Myc tags was used to confirm the presence of L2, PLCδ4, and PLCγ1 in “elutes” and “cleared lysates”. Blots are representative of three independent experiments ( N =3).

Article Snippet: PLCβ4 and PLCδ4 gene edits in selected clonal HeLa cells were further confirmed via sequencing with Plasmidsaurus sequencing services using Oxford Nanopore Technology.

Techniques: Transfection, Over Expression, Construct, Sequencing, FLAG-tag, Plasmid Preparation, Control, Incubation, Magnetic Beads, Co-Immunoprecipitation Assay, Western Blot