miniseq deep sequencing platform (Illumina Inc)
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Miniseq Deep Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 2023 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing+platform+pyrosequencing/MiniSeq+System/pmc12390381-68-21-20
Average 98 stars, based on 2023 article reviews
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Whole Complete genome sequencing:Article Title: Disruption of cAMP Signalling and RNA Regulation Confers Cefiderocol Resistance in Acinetobacter baumannii Article Snippet: Paired-end Illumina DNA libraries were prepared using the Nextera DNA flex library prep kit (Illumina, Australia) with a modification of the starting input of 5 μL genomic DNA (gDNA) with samples of >20 ng/μL. .. WGS of pooled libraries was performed using the Article Title: Benchmarked sequencing methods reveal hidden mosaic variation in the human brain Article Snippet: Library concentrations were further determined by Qubit (Q33226 Invitrogen). .. Libraries were pooled at normalized molecular numbers for a 0.1× shallow WGS as quality control on a Sequencing:Article Title: Impact of TiF 4 Varnish on the Oral Biofilm Microbiome in High-Caries-Risk Patients. Article Snippet: Introduction: Dental caries is a prevalent global disease, influenced by biofilm formation, dietary sugars, and host factors.. Fixed orthodontic appliances increase the risk of noncavitated lesions, highlighting the need for effective prevention.. Fluoride varnishes reduce demineralization and promote remineralization; however, their impact on the oral biofilm microbiome in high-caries-risk patients remains underexplored. Article Title: Human Adenovirus B114 (P7H3F3): A Neglected Re-Emergent Pathogen Long Misclassified as HAdV-B3 With Novel Genomic Variations. Article Snippet: .. Article Title: A Novel HLA-A Allele, HLA-A*24:672, Identified by Next Generation Sequencing. Article Snippet: HLAA*24:672 differs from HLAA*24:424 allele by one nucleotide substitution in codon 73 in exon 2.. The HLA genes are amongst the most polymorphic in the human genome.. We report here a novel HLAA*24 allele, now named HLAA*24:672 that carries one nucleotide substitution in exon 2 when compared with the HLAA*24:424 allele identified by nextgeneration sequencing (NGS) in a Turkish patient. Next-Generation Sequencing:Article Title: Efficient genome editing in ASC-derived porcine lung organoids via GFP-guided enrichment Article Snippet: .. Deep sequencing was conducted using Amplification:Article Title: Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation. Article Snippet: Cells expressing monoallelic HLA class I molecules represent a convenient in vitro model for studying the immunogenicity of tumor protein neoepitopes.. Such models can be generated using endogenous HLA class I-deficient cells.. Here, we describe an effective method for eliminating HLA class I expression in HEK293T cells via simultaneous CRISPR/Cas9 editing of the HLA-A, HLA-B, and HLA-C genes using a single guide RNA. QC/EQA/Proficiency:Article Title: Benchmarked sequencing methods reveal hidden mosaic variation in the human brain Article Snippet: Library concentrations were further determined by Qubit (Q33226 Invitrogen). .. Libraries were pooled at normalized molecular numbers for a 0.1× shallow WGS as quality control on a |
