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miniseq deep sequencing platform  (Illumina Inc)


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    Structured Review

    Illumina Inc miniseq deep sequencing platform
    Miniseq Deep Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 2023 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+platform+pyrosequencing/MiniSeq+System/pmc12390381-68-21-20
    Average 98 stars, based on 2023 article reviews
    miniseq deep sequencing platform - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Whole Complete genome sequencing:

    Article Title: Disruption of cAMP Signalling and RNA Regulation Confers Cefiderocol Resistance in Acinetobacter baumannii
    Article Snippet: Paired-end Illumina DNA libraries were prepared using the Nextera DNA flex library prep kit (Illumina, Australia) with a modification of the starting input of 5 μL genomic DNA (gDNA) with samples of >20 ng/μL. .. WGS of pooled libraries was performed using the Illumina MiniSeq system and the high-output reagent kit (300 cycles). .. Illumina paired-end reads were assembled using SPAdes v3.14.1 with default settings.

    Article Title: Benchmarked sequencing methods reveal hidden mosaic variation in the human brain
    Article Snippet: Library concentrations were further determined by Qubit (Q33226 Invitrogen). .. Libraries were pooled at normalized molecular numbers for a 0.1× shallow WGS as quality control on a MiniSeq System (H3K7TG, Illumina). .. High-fidelity libraries were subjected to 20 × whole-genome sequencing on a NovaSeq 6000 system (Illumina).

    Sequencing:

    Article Title: Impact of TiF 4 Varnish on the Oral Biofilm Microbiome in High-Caries-Risk Patients.
    Article Snippet: Introduction: Dental caries is a prevalent global disease, influenced by biofilm formation, dietary sugars, and host factors.. Fixed orthodontic appliances increase the risk of noncavitated lesions, highlighting the need for effective prevention.. Fluoride varnishes reduce demineralization and promote remineralization; however, their impact on the oral biofilm microbiome in high-caries-risk patients remains underexplored.

    Article Title: Human Adenovirus B114 (P7H3F3): A Neglected Re-Emergent Pathogen Long Misclassified as HAdV-B3 With Novel Genomic Variations.
    Article Snippet: .. Paired‐end sequencing (2 × 150 bp) was performed on an Illumina MiniSeq system with High Output Reagent Cartridge (Illumina, San Diego, CA, USA). ..

    Article Title: A Novel HLA-A Allele, HLA-A*24:672, Identified by Next Generation Sequencing.
    Article Snippet: HLAA*24:672 differs from HLAA*24:424 allele by one nucleotide substitution in codon 73 in exon 2.. The HLA genes are amongst the most polymorphic in the human genome.. We report here a novel HLAA*24 allele, now named HLAA*24:672 that carries one nucleotide substitution in exon 2 when compared with the HLAA*24:424 allele identified by nextgeneration sequencing (NGS) in a Turkish patient.

    Next-Generation Sequencing:

    Article Title: Efficient genome editing in ASC-derived porcine lung organoids via GFP-guided enrichment
    Article Snippet: .. Deep sequencing was conducted using MiniSeq System (Illumina). .. Total RNA was extracted from organoids using the RNeasy Mini Kit (Qiagen, 74104), and residual gDNA was removed using the RNase-Free DNase Set (Qiagen, 79254).

    Amplification:

    Article Title: Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation.
    Article Snippet: Cells expressing monoallelic HLA class I molecules represent a convenient in vitro model for studying the immunogenicity of tumor protein neoepitopes.. Such models can be generated using endogenous HLA class I-deficient cells.. Here, we describe an effective method for eliminating HLA class I expression in HEK293T cells via simultaneous CRISPR/Cas9 editing of the HLA-A, HLA-B, and HLA-C genes using a single guide RNA.

    QC/EQA/Proficiency:

    Article Title: Benchmarked sequencing methods reveal hidden mosaic variation in the human brain
    Article Snippet: Library concentrations were further determined by Qubit (Q33226 Invitrogen). .. Libraries were pooled at normalized molecular numbers for a 0.1× shallow WGS as quality control on a MiniSeq System (H3K7TG, Illumina). .. High-fidelity libraries were subjected to 20 × whole-genome sequencing on a NovaSeq 6000 system (Illumina).



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    Pyrosequencing Inc sequencing platform pyrosequencing
    Mapping of the search strategy, methodology, and results. To assess the role of the CVM in HPV‐associated cervical carcinogenesis, 2223 records were identified in Embase, Medline and Web of Science, of which 951 duplicates were excluded. 1272 articles were screened by title and abstract for relevance and the full texts of 276 articles were assessed for eligibility. Two additional articles were identified in PubMed following the systematic search. A total of 21 observational studies, 4 systematic reviews, and 3 meta‐analyses were included. To assess the diagnostic performance of the CVM in HPV‐associated cervical carcinogenesis, 7 relevant articles were identified in PubMed, of which 3 were excluded. Of the 4 full texts assessed for eligibility, one was excluded as it only assessed the diagnostic performance of bacterial genes (not taxa). The corresponding search strategies for the aforementioned searches are detailed in Table . To identify common metagenomic techniques in microbial research, 11 resources were identified through a focused search of Google and Google Scholar to identify webpages and scholarly articles, respectively. Abbreviations: CVM, cervicovaginal microbiome; HPV, human papillomavirus; ROC, receiver operating characteristic; WMS, whole metagenome <t>sequencing;</t> 16S rRNA, 16S ribosomal RNA.
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    Image Search Results


    Mapping of the search strategy, methodology, and results. To assess the role of the CVM in HPV‐associated cervical carcinogenesis, 2223 records were identified in Embase, Medline and Web of Science, of which 951 duplicates were excluded. 1272 articles were screened by title and abstract for relevance and the full texts of 276 articles were assessed for eligibility. Two additional articles were identified in PubMed following the systematic search. A total of 21 observational studies, 4 systematic reviews, and 3 meta‐analyses were included. To assess the diagnostic performance of the CVM in HPV‐associated cervical carcinogenesis, 7 relevant articles were identified in PubMed, of which 3 were excluded. Of the 4 full texts assessed for eligibility, one was excluded as it only assessed the diagnostic performance of bacterial genes (not taxa). The corresponding search strategies for the aforementioned searches are detailed in Table . To identify common metagenomic techniques in microbial research, 11 resources were identified through a focused search of Google and Google Scholar to identify webpages and scholarly articles, respectively. Abbreviations: CVM, cervicovaginal microbiome; HPV, human papillomavirus; ROC, receiver operating characteristic; WMS, whole metagenome sequencing; 16S rRNA, 16S ribosomal RNA.

    Journal: Journal of Medical Virology

    Article Title: A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus‐Associated Cervical Carcinogenesis

    doi: 10.1002/jmv.70027

    Figure Lengend Snippet: Mapping of the search strategy, methodology, and results. To assess the role of the CVM in HPV‐associated cervical carcinogenesis, 2223 records were identified in Embase, Medline and Web of Science, of which 951 duplicates were excluded. 1272 articles were screened by title and abstract for relevance and the full texts of 276 articles were assessed for eligibility. Two additional articles were identified in PubMed following the systematic search. A total of 21 observational studies, 4 systematic reviews, and 3 meta‐analyses were included. To assess the diagnostic performance of the CVM in HPV‐associated cervical carcinogenesis, 7 relevant articles were identified in PubMed, of which 3 were excluded. Of the 4 full texts assessed for eligibility, one was excluded as it only assessed the diagnostic performance of bacterial genes (not taxa). The corresponding search strategies for the aforementioned searches are detailed in Table . To identify common metagenomic techniques in microbial research, 11 resources were identified through a focused search of Google and Google Scholar to identify webpages and scholarly articles, respectively. Abbreviations: CVM, cervicovaginal microbiome; HPV, human papillomavirus; ROC, receiver operating characteristic; WMS, whole metagenome sequencing; 16S rRNA, 16S ribosomal RNA.

    Article Snippet: , Amplicon sequencing using a sequencing platform (i.e., Pyrosequencing, Illumina MiSeq, Illumina nextSeq, PacBlo Seqel II/IIe, Nanopore) , Sequencing all genomic DNA using a sequencing platform (i.e., Illumina novaSeq, HiSeq ).

    Techniques: Diagnostic Assay, Sequencing

    16S rRNA gene  sequencing  and WMS for CVM characterization.

    Journal: Journal of Medical Virology

    Article Title: A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus‐Associated Cervical Carcinogenesis

    doi: 10.1002/jmv.70027

    Figure Lengend Snippet: 16S rRNA gene sequencing and WMS for CVM characterization.

    Article Snippet: , Amplicon sequencing using a sequencing platform (i.e., Pyrosequencing, Illumina MiSeq, Illumina nextSeq, PacBlo Seqel II/IIe, Nanopore) , Sequencing all genomic DNA using a sequencing platform (i.e., Illumina novaSeq, HiSeq ).

    Techniques: Sequencing, Extraction, Amplification, Nanopore Sequencing, Marker, Bacteria