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Pyrosequencing Inc sequencing platform pyrosequencing

Mapping of the search strategy, methodology, and results. To assess the role of the CVM in HPV‐associated cervical carcinogenesis, 2223 records were identified in Embase, Medline and Web of Science, of which 951 duplicates were excluded. 1272 articles were screened by title and abstract for relevance and the full texts of 276 articles were assessed for eligibility. Two additional articles were identified in PubMed following the systematic search. A total of 21 observational studies, 4 systematic reviews, and 3 meta‐analyses were included. To assess the diagnostic performance of the CVM in HPV‐associated cervical carcinogenesis, 7 relevant articles were identified in PubMed, of which 3 were excluded. Of the 4 full texts assessed for eligibility, one was excluded as it only assessed the diagnostic performance of bacterial genes (not taxa). The corresponding search strategies for the aforementioned searches are detailed in Table . To identify common metagenomic techniques in microbial research, 11 resources were identified through a focused search of Google and Google Scholar to identify webpages and scholarly articles, respectively. Abbreviations: CVM, cervicovaginal microbiome; HPV, human papillomavirus; ROC, receiver operating characteristic; WMS, whole metagenome <t>sequencing;</t> 16S rRNA, 16S ribosomal RNA.

Sequencing Platform Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing+platform+pyrosequencing/sequencing+platforms/pmc11600484-59-5-8
Average 90 stars, based on 1 article reviews

sequencing platform pyrosequencing - by Bioz Stars, 2026-11

90/100 stars

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1) Product Images from "A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus‐Associated Cervical Carcinogenesis"

Article Title: A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus‐Associated Cervical Carcinogenesis

Journal: Journal of Medical Virology

doi: 10.1002/jmv.70027

Figure Legend Snippet: Mapping of the search strategy, methodology, and results. To assess the role of the CVM in HPV‐associated cervical carcinogenesis, 2223 records were identified in Embase, Medline and Web of Science, of which 951 duplicates were excluded. 1272 articles were screened by title and abstract for relevance and the full texts of 276 articles were assessed for eligibility. Two additional articles were identified in PubMed following the systematic search. A total of 21 observational studies, 4 systematic reviews, and 3 meta‐analyses were included. To assess the diagnostic performance of the CVM in HPV‐associated cervical carcinogenesis, 7 relevant articles were identified in PubMed, of which 3 were excluded. Of the 4 full texts assessed for eligibility, one was excluded as it only assessed the diagnostic performance of bacterial genes (not taxa). The corresponding search strategies for the aforementioned searches are detailed in Table . To identify common metagenomic techniques in microbial research, 11 resources were identified through a focused search of Google and Google Scholar to identify webpages and scholarly articles, respectively. Abbreviations: CVM, cervicovaginal microbiome; HPV, human papillomavirus; ROC, receiver operating characteristic; WMS, whole metagenome sequencing; 16S rRNA, 16S ribosomal RNA.

Techniques Used: Diagnostic Assay, Sequencing

Figure Legend Snippet: 16S rRNA gene sequencing and WMS for CVM characterization.

Techniques Used: Sequencing, Extraction, Amplification, Nanopore Sequencing, Marker, Bacteria

Sequencing:

Article Title: Simultaneous analysis of seven 16S rRNA hypervariable gene regions increases efficiency in marine bacterial diversity detection.
Article Snippet: .. The commonality of the above mentioned (and also other) studies is that they usually analyse only one or two full hypervariable regions to infer bacterial diversity because mainstream sequencing platforms (lllumina and previously Pyrosequencing and IonTorrent platforms) are limited by their capacity to sequence relatively short regions of DNA (Kerrigan et al., 2019). .. Although this has changed with the emergence of long-read sequencing technologies commercialized by Oxford Nanopore Technologies and Pacific Biosciences, short-read platforms still offer better costefficiency and do not require specific library preparation techniques such as amplicon circularisation in order to achieve relatively low error rates (Santos et al., 2020).

Article Title: Improved Selection of Internal Transcribed Spacer-Specific Primers Enables Quantitative, Ultra-High-Throughput Profiling of Fungal Communities
Article Snippet: Pyrosequencing (4) has been used most frequently to study fungal communities of foods (5), the human gut (6), and soils (7), as 454 Life Sciences pyrosequencing platforms yield longer sequences (400 to 600 bp), which are presumably necessary for accurate identification of fungi. .. However, the greater sequencing coverage of Illumina sequencing platforms ( 108 reads/lane on the HiSeq2000) compared to pyrosequencing ( 106 reads/run) enables multiplexing of more samples per run and a magnitudes-lower cost per sample, popularizing these platforms for analysis of bacterial communities. .. No studies to date have utilized the superior coverage of Illumina sequencing platforms to analyze fungal communities, presumably because the shorter read length may compromise taxonomic resolution.

Article Title: The Role of Non-Typeable Haemophilus influenzae Biofilms in Chronic Obstructive Pulmonary Disease
Article Snippet: .. Disparity can also be attributed to having no standard methodology, including sampling and sequencing inconsistencies, for example using different 16S hypervariable regions or using Illumina or Pyrosequencing platforms, that may yield different results when quantifying the abundance of bacteria taxa. ..

Article Title: Agnostic Sequencing for Detection of Viral Pathogens.
Article Snippet: aDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada bBugSeq Bioinformatics Inc., Vancouver, British Columbia, Canada cDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada dBritish Columbia Centre for Disease Control, Vancouver, British Columbia, Canada eSchool of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada

Article Title: A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus‐Associated Cervical Carcinogenesis
Article Snippet: .. , Amplicon sequencing using a sequencing platform (i.e., Pyrosequencing, Illumina MiSeq, Illumina nextSeq, PacBlo Seqel II/IIe, Nanopore) , Sequencing all genomic DNA using a sequencing platform (i.e., Illumina novaSeq, HiSeq ). .. , Taxonomic assignment using curated 16S rRNA databases , Taxonomic assignment using nonredundant databases or marker databases (metaphlan).

Article Title: Progress in Research on the Gut Microflora of the Red Panda ( Ailurus fulgens )
Article Snippet: .. Sequencing platforms based on pyrosequencing technology have been widely employed, as have serial Illumina sequencing instruments. ..

Article Title: Indel and Carryforward Correction (ICC): a new analysis approach for processing 454 pyrosequencing data
Article Snippet: .. Pyrosequencing error rate is largely dependent on the sequencing platform, PCR amplification and sequence context ( Gilles et al. , 2011 ; Huse et al. , 2007 ; Shao et al. , 2013 ; Vandenbroucke et al. , 2011 ; Wang et al. , 2007 ). .. Wang et al. (2007) measured mismatch error rates using a Roche 454 GS20 sequencing platform and found that mismatches were six times more frequent in homopolymeric regions (0.0044) than in non-homopolymeric regions (0.0007).

Multiplexing:

Sampling:

Bacteria:

Produced:

Amplification:

Nanopore Sequencing:

Polymerase Chain Reaction:

other:

Article Title: Small-Magnitude Effect Sizes in Epigenetic End Points are Important in Children’s Environmental Health Studies: The Children’s Environmental Health and Disease Prevention Research Center’s Epigenetics Working Group
Article Snippet: Pyrosequencing (PSQ) is a “sequencing by synthesis” platform that can quantify the proportion of individual nucleotides at a given position in a sequence [e.g., single-nucleotide polymorphisms (SNPs) or, relevant herein, cytosine versus thymine], providing the ability to detect small differences in methylation among samples or groups due to much greater depth of coverage than Sanger sequencing ( ).

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