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Novogene rna sequencing libraries
Rna Sequencing Libraries, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing+library/libraries+rna+seq/pm42269550-91-0-8
Average 86 stars, based on 1 article reviews
rna sequencing libraries - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

RNA sequencing:

Article Title: Transcription factor CCTF1 plays a decisive role in regulating the proteasome activity during archaeal cell division
Article Snippet: Total RNA was extracted using the Trizol reagent (Ambion, Austin, TX, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used to assess the total amounts and integrity of the RNA. .. Transcriptomic analysis was carried out by Novogene (Beijing, China), with approximately 3 μg of high-quality RNA per sample used for the construction of RNA-Seq libraries. ..

Construct:

Article Title: Transcriptomics analyses reveal microgravity triggers estrogen synthesis amidst degeneration and aging in human ovarian granulosa cells.
Article Snippet: Advances in aerospace technology are making space travel accessible to more people.. However, the space environment, especially unavoidable microgravity, poses health risks that are particularly concerning for women’s reproductive endocrine health.. While female estrogen secretion dysregulation represents a core concern, research on human ovarian granulosa cells, the primary site of estrogen synthesis, remains critically underdeveloped due to a scarcity of sequencing data, hindering both mechanistic investigation and intervention development.

Article Title: Functional evolution and rewiring of the UVR8-BES1/BIM1 module underpin the refined UV-B responses during plant terrestrialization.
Article Snippet: .. RNA-seq libraries were 496 constructed and sequenced by Novogene Bioinformatics Technology Co., Ltd (Beijing, 497 China). .. Paired-end sequencing (150 bp) was performed using the Illumina NovaSeq 498 6000 platform.

RNA Sequencing:

Article Title: Nonsense-mediated mRNA decay orchestrates neuronal migration and cortical lamination while modulating Reelin and ciliary gene regulatory networks
Article Snippet: .. All premade RNA-seq libraries were sequenced using NovaSeq X plus platform by Novogene Corporation Inc., generating >120M reads per sample for subsequent analysis. ..

Article Title: Functional evolution and rewiring of the UVR8-BES1/BIM1 module underpin the refined UV-B responses during plant terrestrialization.
Article Snippet: .. RNA-seq libraries were 496 constructed and sequenced by Novogene Bioinformatics Technology Co., Ltd (Beijing, 497 China). .. Paired-end sequencing (150 bp) was performed using the Illumina NovaSeq 498 6000 platform.

Article Title: Identification of Biomarker in Kidney Stone Disease by Integrating Transcriptomics and Olink Proteomics: A Case–Control Study
Article Snippet: .. RNA-seq libraries were prepared and sequenced by Novogene Bioinformatics Technology Co. Ltd (Beijing, China). ..

Article Title: Successive waves of transcriptional repression and de-repression license cell cycle progression in an archaeon
Article Snippet: Total RNA was extracted using the Trizol reagent (Ambion, Austin, TX, USA), and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used to assess the total amounts and integrity of the RNA. .. Transcriptomic analysis was carried out by Novogene (Beijing, China), with ~3 μg of high-quality RNA per sample used for the construction of RNA-seq libraries. ..

Article Title: Involvement of monocyte/macrophage NLRP3-IL-1β signaling in the pathogenesis of myasthenia gravis.
Article Snippet: Background: Myasthenia gravis is an autoimmune disorder mediated by pathogenic autoantibodies targeting the neuromuscular junction.. Although the role of adaptive immunity is well-established, the involvement of innate immune cells, particularly monocytes and macrophages, in myasthenia gravis pathogenesis remains unclear.. This study investigated whether the NLRP3 inflammasome-caspase-1-IL-1β pathway in monocytes and macrophages contributes to disease development in myasthenia gravis and its experimental model. Methods: Experimental autoimmune myasthenia gravis was induced in Lewis rats by immunization with AChR97–116 peptide.

Sequencing:

Article Title: Methionine, Not S -adenosylmethionine, Acts as a Primary Metabolic Stress Signal for Chromatin Remodeling
Article Snippet: .. RNA libraries were prepared by Novogene followed by paired end 2×150 non-directional sequencing on their NovaSeq X Plus Series platform. .. All analyses were performed by Novogene using their NovoMagic interactive programming.



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Image Search Results


Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X 0,16S values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.

Journal: Environmental Microbiology

Article Title: The Adult Aedes aegypti Bacterial Microbiota is Robust to Infection with the Obligate Microsporidian Parasite Edhazardia aedis

doi: 10.1111/1462-2920.70437

Figure Lengend Snippet: Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X 0,16S values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.

Article Snippet: Amplicons were submitted to NC State Genomic Sciences Laboratory for subsequent library preparation following the Illumina 16S Metagenomic Sequencing Library Preparation guide ( 16S Metagenomic Sequencing Library Preparation: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System ).

Techniques: Infection, Transformation Assay, Analysis, Real-time Polymerase Chain Reaction

Normalized CDKN2A transcript counts from RNA sequencing according to HPV/p16 status in our cohort

Journal: bioRxiv

Article Title: Dysregulation of the p16–CDK6 axis characterizes HPV-unrelated p16-positive oropharyngeal carcinoma

doi: 10.64898/2026.08.17.743010

Figure Lengend Snippet: Normalized CDKN2A transcript counts from RNA sequencing according to HPV/p16 status in our cohort

Article Snippet: Sequencing libraries were generated with the Twist EF 2.0 Library Preparation Kit (Twist Biosciences, South San Francisco, CA, USA).

Techniques: RNA Sequencing

(A) STRING network visualization of genes in the “Pathways in cancer” pathway that were commonly upregulated in HPV-unrelated OPC. (B) Schematic diagram of the p16–RB pathway. (C) CDK6 expression in RNA sequencing data stratified by HPV and CDKN2A status in the TCGA dataset. (D) Representative immunohistochemistry images of CDK6 staining. Scale bar, 200 μm. (E) IHC scores for CDK6 staining. (F) Western blot showing p16 expression in RPE1 cells with doxycycline-inducible CDK6 expression. (G) Western blot showing CDK6 expression following p16 transduction in CDKN2A-deficient FaDu cells. (H) Proportion of CDK6 expression levels in leukoplakia. CDK6 immunohistochemical staining was evaluated using a scoring system, and the distribution of scores is shown by histopathological grade. ND/LGD, non-dysplastic/low-grade dysplasia; HGD, high-grade dysplasia; SCC, squamous cell carcinoma in situ or carcinoma with minimal invasion. (I) HE and immunohistochemical images of oral leukoplakia with high-grade dysplasia showing p16 expression. Scale bars, 50 μm. (J) Clonal expansion of CDKN2A-deficient cells in CDK6-overexpressing RPE1 cells. The fraction of CDKN2A indels was quantified over time in RPE1 cells expressing the CDK6 (R31C) mutant in a doxycycline-dependent manner. DOX, doxycycline. Statistical analyses were performed using the Mann–Whitney U test in (C) and (E), and Welch’s t-test in (J). **p < 0.01, ***p < 0.001. n.s., not significant.

Journal: bioRxiv

Article Title: Dysregulation of the p16–CDK6 axis characterizes HPV-unrelated p16-positive oropharyngeal carcinoma

doi: 10.64898/2026.08.17.743010

Figure Lengend Snippet: (A) STRING network visualization of genes in the “Pathways in cancer” pathway that were commonly upregulated in HPV-unrelated OPC. (B) Schematic diagram of the p16–RB pathway. (C) CDK6 expression in RNA sequencing data stratified by HPV and CDKN2A status in the TCGA dataset. (D) Representative immunohistochemistry images of CDK6 staining. Scale bar, 200 μm. (E) IHC scores for CDK6 staining. (F) Western blot showing p16 expression in RPE1 cells with doxycycline-inducible CDK6 expression. (G) Western blot showing CDK6 expression following p16 transduction in CDKN2A-deficient FaDu cells. (H) Proportion of CDK6 expression levels in leukoplakia. CDK6 immunohistochemical staining was evaluated using a scoring system, and the distribution of scores is shown by histopathological grade. ND/LGD, non-dysplastic/low-grade dysplasia; HGD, high-grade dysplasia; SCC, squamous cell carcinoma in situ or carcinoma with minimal invasion. (I) HE and immunohistochemical images of oral leukoplakia with high-grade dysplasia showing p16 expression. Scale bars, 50 μm. (J) Clonal expansion of CDKN2A-deficient cells in CDK6-overexpressing RPE1 cells. The fraction of CDKN2A indels was quantified over time in RPE1 cells expressing the CDK6 (R31C) mutant in a doxycycline-dependent manner. DOX, doxycycline. Statistical analyses were performed using the Mann–Whitney U test in (C) and (E), and Welch’s t-test in (J). **p < 0.01, ***p < 0.001. n.s., not significant.

Article Snippet: Sequencing libraries were generated with the Twist EF 2.0 Library Preparation Kit (Twist Biosciences, South San Francisco, CA, USA).

Techniques: Expressing, RNA Sequencing, Immunohistochemistry, Staining, Western Blot, Transduction, Immunohistochemical staining, In Situ, Mutagenesis, MANN-WHITNEY

(A) Schematic illustration of the CDKN2A alteration inferred from oncopanel sequencing in Case IV shown in . (B) IGV view of RNA sequencing read alignments flanking the CDKN2A deletion in the same case.

Journal: bioRxiv

Article Title: Dysregulation of the p16–CDK6 axis characterizes HPV-unrelated p16-positive oropharyngeal carcinoma

doi: 10.64898/2026.08.17.743010

Figure Lengend Snippet: (A) Schematic illustration of the CDKN2A alteration inferred from oncopanel sequencing in Case IV shown in . (B) IGV view of RNA sequencing read alignments flanking the CDKN2A deletion in the same case.

Article Snippet: Sequencing libraries were generated with the Twist EF 2.0 Library Preparation Kit (Twist Biosciences, South San Francisco, CA, USA).

Techniques: Sequencing, RNA Sequencing