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sequencing data long read  (Pacific Biosciences)

 
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    Pacific Biosciences sequencing data long read
    Sequencing Data Long Read, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+data+long+read/long+read+sequencing/pm41099697-115-9-18
    Average 86 stars, based on 1 article reviews
    sequencing data long read - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy.
    Article Snippet: .. Moreover, if the researcher seeks only to obtain intact nuclei for other downstream applications, such as isolation of high-molecular-weight DNA for long-read sequencing (e.g., Pacific Biosciences or Oxford Nanopore), the sorting step can be replaced by two or three centrifugation steps to wash nuclei (500 RCF x 5 min at 4 ◦ C) until the supernatant is clear, followed by a traditional cetyltrimethylammonium bromide (CTAB) extraction method. ..

    Sequencing:

    Article Title: Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy.
    Article Snippet: .. Moreover, if the researcher seeks only to obtain intact nuclei for other downstream applications, such as isolation of high-molecular-weight DNA for long-read sequencing (e.g., Pacific Biosciences or Oxford Nanopore), the sorting step can be replaced by two or three centrifugation steps to wash nuclei (500 RCF x 5 min at 4 ◦ C) until the supernatant is clear, followed by a traditional cetyltrimethylammonium bromide (CTAB) extraction method. ..

    Article Title: Allos: an integrated Python toolkit for isoform-level single-cell and spatial in-situ transcriptomics
    Article Snippet: .. Long-read (LR) sequencing technologies, such as Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have the potential to resolve full-length transcripts and novel isoforms in single-cell and in situ capture assays. ..

    Article Title: A gradient green-beard gene in fission yeast
    Article Snippet: .. Genomic DNA was sequenced using the Pacific Biosciences (PacBio) long-read sequencing technology provided by Frasergen Biotechnology (Wuhan, China). ..

    Article Title: Benchmarking of sequencing technologies defines optimal strategies for genetic variants detection in a human genome.
    Article Snippet: .. Short-read sequencing remains widely used due to its high base accuracy, throughput, and cost efficiency; however, its limited ability to resolve repetitive and structurally complex regions has accelerated adoption of long-read sequencing platforms, including those from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT). ..

    Article Title: ERGA-BGE reference genome of the Azores Bullfinch - Pyrrhula murina Godman, 1866: an IUCN Vulnerable Species endemic to a single island in the Azores Archipelago (Portugal)
    Article Snippet: .. ERGA’s sequencing strategy includes Oxford Nanopore Technology (ONT) and/or Pacific Biosciences (PacBio) for long-read sequencing, along with Hi-C sequencing for chromosomal architecture, Illumina Paired-End (PE) for k-mer profiling and polishing (i.e. recommended for ONT-only assemblies), to facilitate genome assembly, as well as Illumina RNA sequencing for transcriptomic profiling and annotation. ..

    Article Title: Circles That Mutate
    Article Snippet: .. The advent of long-read sequencing platforms, principally Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), has transformed the detection of structural mutations in circular DNA. ..

    Centrifugation:

    Article Title: Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy.
    Article Snippet: .. Moreover, if the researcher seeks only to obtain intact nuclei for other downstream applications, such as isolation of high-molecular-weight DNA for long-read sequencing (e.g., Pacific Biosciences or Oxford Nanopore), the sorting step can be replaced by two or three centrifugation steps to wash nuclei (500 RCF x 5 min at 4 ◦ C) until the supernatant is clear, followed by a traditional cetyltrimethylammonium bromide (CTAB) extraction method. ..

    Extraction:

    Article Title: Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy.
    Article Snippet: .. Moreover, if the researcher seeks only to obtain intact nuclei for other downstream applications, such as isolation of high-molecular-weight DNA for long-read sequencing (e.g., Pacific Biosciences or Oxford Nanopore), the sorting step can be replaced by two or three centrifugation steps to wash nuclei (500 RCF x 5 min at 4 ◦ C) until the supernatant is clear, followed by a traditional cetyltrimethylammonium bromide (CTAB) extraction method. ..

    Single Cell:

    Article Title: Allos: an integrated Python toolkit for isoform-level single-cell and spatial in-situ transcriptomics
    Article Snippet: .. Long-read (LR) sequencing technologies, such as Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have the potential to resolve full-length transcripts and novel isoforms in single-cell and in situ capture assays. ..

    In Situ:

    Article Title: Allos: an integrated Python toolkit for isoform-level single-cell and spatial in-situ transcriptomics
    Article Snippet: .. Long-read (LR) sequencing technologies, such as Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have the potential to resolve full-length transcripts and novel isoforms in single-cell and in situ capture assays. ..

    other:

    Article Title: Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy
    Article Snippet: Moreover, if the researcher seeks only to obtain intact nuclei for other downstream applications, such as isolation of high-molecular-weight DNA for long-read sequencing (e.g., Pacific Biosciences or Oxford Nanopore), the sorting step can be replaced by two or three centrifugation steps to wash nuclei (500 RCF x 5 min at 4°C) until the supernatant is clear, followed by a traditional cetyltrimethylammonium bromide (CTAB) extraction method., We have successfully used this nuclei extraction method in a recalcitrant plant species from the Atacama Desert (Región de Atacama, Chile), obtaining high-molecular-weight DNA ( : reproducibility of nuclei isolation in other species).

    Hi-C:

    Article Title: ERGA-BGE reference genome of the Azores Bullfinch - Pyrrhula murina Godman, 1866: an IUCN Vulnerable Species endemic to a single island in the Azores Archipelago (Portugal)
    Article Snippet: .. ERGA’s sequencing strategy includes Oxford Nanopore Technology (ONT) and/or Pacific Biosciences (PacBio) for long-read sequencing, along with Hi-C sequencing for chromosomal architecture, Illumina Paired-End (PE) for k-mer profiling and polishing (i.e. recommended for ONT-only assemblies), to facilitate genome assembly, as well as Illumina RNA sequencing for transcriptomic profiling and annotation. ..

    RNA Sequencing:

    Article Title: ERGA-BGE reference genome of the Azores Bullfinch - Pyrrhula murina Godman, 1866: an IUCN Vulnerable Species endemic to a single island in the Azores Archipelago (Portugal)
    Article Snippet: .. ERGA’s sequencing strategy includes Oxford Nanopore Technology (ONT) and/or Pacific Biosciences (PacBio) for long-read sequencing, along with Hi-C sequencing for chromosomal architecture, Illumina Paired-End (PE) for k-mer profiling and polishing (i.e. recommended for ONT-only assemblies), to facilitate genome assembly, as well as Illumina RNA sequencing for transcriptomic profiling and annotation. ..

    Transformation Assay:

    Article Title: Circles That Mutate
    Article Snippet: .. The advent of long-read sequencing platforms, principally Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), has transformed the detection of structural mutations in circular DNA. ..



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    Image Search Results


    A. Example IGV views of a germline and postzygotic mutation in HiFi read data. B. The number of autosomal de novo germline and postzygotic SNVs, insertions and deletions <50 bp, and tandem repeat mutations observed in each sample. Sibling pairs are grouped by family and highlighted in blue, with the proband above the sibling. C. Upset plot of origin assignment shows concordance between HiFi haplotypes, ONT haplotypes, and allele balance. D. Allele balance distribution for autosomal germline and postzygotic SNVs across PacBio HiFi, Illumina, and ONT read data. E. Distribution of the size of autosomal insertions, deletions, and tandem repeat mutations.

    Journal: bioRxiv

    Article Title: Long-read sequencing of trios reveals increased germline and postzygotic mutation rates in repetitive DNA

    doi: 10.1101/2025.07.18.665621

    Figure Lengend Snippet: A. Example IGV views of a germline and postzygotic mutation in HiFi read data. B. The number of autosomal de novo germline and postzygotic SNVs, insertions and deletions <50 bp, and tandem repeat mutations observed in each sample. Sibling pairs are grouped by family and highlighted in blue, with the proband above the sibling. C. Upset plot of origin assignment shows concordance between HiFi haplotypes, ONT haplotypes, and allele balance. D. Allele balance distribution for autosomal germline and postzygotic SNVs across PacBio HiFi, Illumina, and ONT read data. E. Distribution of the size of autosomal insertions, deletions, and tandem repeat mutations.

    Article Snippet: We leveraged long-read Pacific Biosciences high-fidelity (HiFi) sequencing data derived from blood for variant discovery, and both long-read Oxford Nanopore Technologies (ONT) and short-read Illumina data for validation purposes.

    Techniques: Mutagenesis

    Encoding of example deletions by different sequencing methods. Deletion of chr12 121,792,102–121,792,155 could be detected correctly by all sequencing methods ( A ). Deletion of chr5, 114,989,663–114,999,300 could be detected correctly only by long-read sequencing methods ( B ). Values in brackets represent scores predicted by ConsensuSV-ONT, but only for long-read sequencing.

    Journal: Scientific Reports

    Article Title: ConsensuSV-ONT – A modern method for accurate structural variant calling

    doi: 10.1038/s41598-025-01486-1

    Figure Lengend Snippet: Encoding of example deletions by different sequencing methods. Deletion of chr12 121,792,102–121,792,155 could be detected correctly by all sequencing methods ( A ). Deletion of chr5, 114,989,663–114,999,300 could be detected correctly only by long-read sequencing methods ( B ). Values in brackets represent scores predicted by ConsensuSV-ONT, but only for long-read sequencing.

    Article Snippet: The solution is complete in its form and is ready to use not only by computer scientists but accessible and easy to use for everyone working with Oxford Nanopore long-read sequencing data.

    Techniques: Sequencing

    Encoding of merged mutation by different sequencing methods.

    Journal: Scientific Reports

    Article Title: ConsensuSV-ONT – A modern method for accurate structural variant calling

    doi: 10.1038/s41598-025-01486-1

    Figure Lengend Snippet: Encoding of merged mutation by different sequencing methods.

    Article Snippet: The solution is complete in its form and is ready to use not only by computer scientists but accessible and easy to use for everyone working with Oxford Nanopore long-read sequencing data.

    Techniques: Mutagenesis, Sequencing