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Oxford Nanopore r10.4 longread sequencing
R10.4 Longread Sequencing, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing+data+long+read/oxford+nanopore+r10+4+long+read+sequencing/pm38592968-294-2-0
Average 90 stars, based on 1 article reviews
r10.4 longread sequencing - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Sequencing:

Article Title: Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?
Article Snippet: In a study by , 59 human nasal swab samples were analyzed using both Illumina MiSeq and Oxford Nanopore Technologies 16S rRNA gene sequencing platforms.

Article Title: Tracking Antimicrobial Resistant Organisms Timely: a workflow validation study for successive core-genome SNP-based nosocomial transmission analysis
Article Snippet: However, batch sequencing is time-consuming as it requires the collection of a large number of samples for a single analysis, making it challenging to provide cgSNP analysis results promptly and timely enough to influence IPC intervention., The latest MinION/Flongle technology [R10.4.1; Oxford Nanopore Technologies (ONT), Oxford, UK] offers a cost-effective and scalable solution for daily, single-strain WGS and recent improvements have addressed its previously lower basecalling accuracy compared with Illumina.

Article Title: Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae bloodstream infections from Aberdeen, Scotland, and their comparison to isolates from England.
Article Snippet: We used the MicrobesNG Hybrid service that offers both long- and short- read sequencing and combines data from the latest R10.4.1 chemistry from Oxford Nanopore Technologies with 2×250 bp Illumina reads to obtain the highest- quality assemblies for our bacterial genomes.

Article Title: Investigating RNA dynamics from single molecule transcriptomes.
Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions.

Article Title: Co-infection with Proteus mirabilis and extraintestinal pathogenic Escherichia coli aggravated liver injury in chickens by disrupting the jejunal barrier
Article Snippet: The contents of the jejunum ( n = 3) were sent to Wuhan Benagen Technology Company Limited (Wuhan, China) for 16S rRNA amplicon Oxford Nanopore Technologies (ONT) sequencing.

Article Title: Investigating the Microbial Dynamics of Hermetia illucens Powder Throughout Rearing and Processing: An Integrated Approach Using Cultural and Metabarcoding Methods.
Article Snippet: Advances in sequencing technologies refine these methods, with Oxford Nanopore R10.4.1 and PacBio Sequel II improving species-level resolution in environmental microbiomes [94].

Article Title: Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient.
Article Snippet: Although Nanopore has a relatively higher error rate compared to other short-read sequencing platforms111, the newest Oxford Nanopore R10.4.1 technology we used has been proven reliable in enabling accurate species-level assignments in microbial community profiling112.

Article Title: Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient
Article Snippet: Although Nanopore has a relatively higher error rate compared to other short-read sequencing platforms , the newest Oxford Nanopore R10.4.1 technology we used has been proven reliable in enabling accurate species-level assignments in microbial community profiling .

Amplification:

Article Title: Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?
Article Snippet: In a study by , 59 human nasal swab samples were analyzed using both Illumina MiSeq and Oxford Nanopore Technologies 16S rRNA gene sequencing platforms.

Article Title: Tracking Antimicrobial Resistant Organisms Timely: a workflow validation study for successive core-genome SNP-based nosocomial transmission analysis
Article Snippet: However, batch sequencing is time-consuming as it requires the collection of a large number of samples for a single analysis, making it challenging to provide cgSNP analysis results promptly and timely enough to influence IPC intervention., The latest MinION/Flongle technology [R10.4.1; Oxford Nanopore Technologies (ONT), Oxford, UK] offers a cost-effective and scalable solution for daily, single-strain WGS and recent improvements have addressed its previously lower basecalling accuracy compared with Illumina.

Article Title: Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae bloodstream infections from Aberdeen, Scotland, and their comparison to isolates from England.
Article Snippet: We used the MicrobesNG Hybrid service that offers both long- and short- read sequencing and combines data from the latest R10.4.1 chemistry from Oxford Nanopore Technologies with 2×250 bp Illumina reads to obtain the highest- quality assemblies for our bacterial genomes.

Article Title: Investigating RNA dynamics from single molecule transcriptomes.
Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions.

Article Title: Co-infection with Proteus mirabilis and extraintestinal pathogenic Escherichia coli aggravated liver injury in chickens by disrupting the jejunal barrier
Article Snippet: The contents of the jejunum ( n = 3) were sent to Wuhan Benagen Technology Company Limited (Wuhan, China) for 16S rRNA amplicon Oxford Nanopore Technologies (ONT) sequencing.

Article Title: Investigating the Microbial Dynamics of Hermetia illucens Powder Throughout Rearing and Processing: An Integrated Approach Using Cultural and Metabarcoding Methods.
Article Snippet: Advances in sequencing technologies refine these methods, with Oxford Nanopore R10.4.1 and PacBio Sequel II improving species-level resolution in environmental microbiomes [94].

Article Title: Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient.
Article Snippet: Although Nanopore has a relatively higher error rate compared to other short-read sequencing platforms111, the newest Oxford Nanopore R10.4.1 technology we used has been proven reliable in enabling accurate species-level assignments in microbial community profiling112.

Article Title: Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient
Article Snippet: Although Nanopore has a relatively higher error rate compared to other short-read sequencing platforms , the newest Oxford Nanopore R10.4.1 technology we used has been proven reliable in enabling accurate species-level assignments in microbial community profiling .

Metaproteomics:

Article Title: Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?
Article Snippet: In a study by , 59 human nasal swab samples were analyzed using both Illumina MiSeq and Oxford Nanopore Technologies 16S rRNA gene sequencing platforms.

Article Title: Tracking Antimicrobial Resistant Organisms Timely: a workflow validation study for successive core-genome SNP-based nosocomial transmission analysis
Article Snippet: However, batch sequencing is time-consuming as it requires the collection of a large number of samples for a single analysis, making it challenging to provide cgSNP analysis results promptly and timely enough to influence IPC intervention., The latest MinION/Flongle technology [R10.4.1; Oxford Nanopore Technologies (ONT), Oxford, UK] offers a cost-effective and scalable solution for daily, single-strain WGS and recent improvements have addressed its previously lower basecalling accuracy compared with Illumina.

Article Title: Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae bloodstream infections from Aberdeen, Scotland, and their comparison to isolates from England.
Article Snippet: We used the MicrobesNG Hybrid service that offers both long- and short- read sequencing and combines data from the latest R10.4.1 chemistry from Oxford Nanopore Technologies with 2×250 bp Illumina reads to obtain the highest- quality assemblies for our bacterial genomes.

Article Title: Investigating RNA dynamics from single molecule transcriptomes.
Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions.

Article Title: Co-infection with Proteus mirabilis and extraintestinal pathogenic Escherichia coli aggravated liver injury in chickens by disrupting the jejunal barrier
Article Snippet: The contents of the jejunum ( n = 3) were sent to Wuhan Benagen Technology Company Limited (Wuhan, China) for 16S rRNA amplicon Oxford Nanopore Technologies (ONT) sequencing.

Article Title: Investigating the Microbial Dynamics of Hermetia illucens Powder Throughout Rearing and Processing: An Integrated Approach Using Cultural and Metabarcoding Methods.
Article Snippet: Advances in sequencing technologies refine these methods, with Oxford Nanopore R10.4.1 and PacBio Sequel II improving species-level resolution in environmental microbiomes [94].

Article Title: Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient.
Article Snippet: Although Nanopore has a relatively higher error rate compared to other short-read sequencing platforms111, the newest Oxford Nanopore R10.4.1 technology we used has been proven reliable in enabling accurate species-level assignments in microbial community profiling112.

Article Title: Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient
Article Snippet: Although Nanopore has a relatively higher error rate compared to other short-read sequencing platforms , the newest Oxford Nanopore R10.4.1 technology we used has been proven reliable in enabling accurate species-level assignments in microbial community profiling .



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A. Example IGV views of a germline and postzygotic mutation in <t>HiFi</t> read data. B. The number of autosomal de novo germline and postzygotic SNVs, insertions and deletions <50 bp, and tandem repeat mutations observed in each sample. Sibling pairs are grouped by family and highlighted in blue, with the proband above the sibling. C. Upset plot of origin assignment shows concordance between HiFi haplotypes, ONT haplotypes, and allele balance. D. Allele balance distribution for autosomal germline and postzygotic SNVs across PacBio HiFi, Illumina, and ONT read data. E. Distribution of the size of autosomal insertions, deletions, and tandem repeat mutations.
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A. Example IGV views of a germline and postzygotic mutation in <t>HiFi</t> read data. B. The number of autosomal de novo germline and postzygotic SNVs, insertions and deletions <50 bp, and tandem repeat mutations observed in each sample. Sibling pairs are grouped by family and highlighted in blue, with the proband above the sibling. C. Upset plot of origin assignment shows concordance between HiFi haplotypes, ONT haplotypes, and allele balance. D. Allele balance distribution for autosomal germline and postzygotic SNVs across PacBio HiFi, Illumina, and ONT read data. E. Distribution of the size of autosomal insertions, deletions, and tandem repeat mutations.
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Encoding of example deletions by different <t>sequencing</t> methods. Deletion of chr12 121,792,102–121,792,155 could be detected correctly by all sequencing methods ( A ). Deletion of chr5, 114,989,663–114,999,300 could be detected correctly only by long-read sequencing methods ( B ). Values in brackets represent scores predicted by ConsensuSV-ONT, but only for long-read sequencing.
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Image Search Results


A. Example IGV views of a germline and postzygotic mutation in HiFi read data. B. The number of autosomal de novo germline and postzygotic SNVs, insertions and deletions <50 bp, and tandem repeat mutations observed in each sample. Sibling pairs are grouped by family and highlighted in blue, with the proband above the sibling. C. Upset plot of origin assignment shows concordance between HiFi haplotypes, ONT haplotypes, and allele balance. D. Allele balance distribution for autosomal germline and postzygotic SNVs across PacBio HiFi, Illumina, and ONT read data. E. Distribution of the size of autosomal insertions, deletions, and tandem repeat mutations.

Journal: bioRxiv

Article Title: Long-read sequencing of trios reveals increased germline and postzygotic mutation rates in repetitive DNA

doi: 10.1101/2025.07.18.665621

Figure Lengend Snippet: A. Example IGV views of a germline and postzygotic mutation in HiFi read data. B. The number of autosomal de novo germline and postzygotic SNVs, insertions and deletions <50 bp, and tandem repeat mutations observed in each sample. Sibling pairs are grouped by family and highlighted in blue, with the proband above the sibling. C. Upset plot of origin assignment shows concordance between HiFi haplotypes, ONT haplotypes, and allele balance. D. Allele balance distribution for autosomal germline and postzygotic SNVs across PacBio HiFi, Illumina, and ONT read data. E. Distribution of the size of autosomal insertions, deletions, and tandem repeat mutations.

Article Snippet: We leveraged long-read Pacific Biosciences high-fidelity (HiFi) sequencing data derived from blood for variant discovery, and both long-read Oxford Nanopore Technologies (ONT) and short-read Illumina data for validation purposes.

Techniques: Mutagenesis

Encoding of example deletions by different sequencing methods. Deletion of chr12 121,792,102–121,792,155 could be detected correctly by all sequencing methods ( A ). Deletion of chr5, 114,989,663–114,999,300 could be detected correctly only by long-read sequencing methods ( B ). Values in brackets represent scores predicted by ConsensuSV-ONT, but only for long-read sequencing.

Journal: Scientific Reports

Article Title: ConsensuSV-ONT – A modern method for accurate structural variant calling

doi: 10.1038/s41598-025-01486-1

Figure Lengend Snippet: Encoding of example deletions by different sequencing methods. Deletion of chr12 121,792,102–121,792,155 could be detected correctly by all sequencing methods ( A ). Deletion of chr5, 114,989,663–114,999,300 could be detected correctly only by long-read sequencing methods ( B ). Values in brackets represent scores predicted by ConsensuSV-ONT, but only for long-read sequencing.

Article Snippet: The solution is complete in its form and is ready to use not only by computer scientists but accessible and easy to use for everyone working with Oxford Nanopore long-read sequencing data.

Techniques: Sequencing

Encoding of merged mutation by different sequencing methods.

Journal: Scientific Reports

Article Title: ConsensuSV-ONT – A modern method for accurate structural variant calling

doi: 10.1038/s41598-025-01486-1

Figure Lengend Snippet: Encoding of merged mutation by different sequencing methods.

Article Snippet: The solution is complete in its form and is ready to use not only by computer scientists but accessible and easy to use for everyone working with Oxford Nanopore long-read sequencing data.

Techniques: Mutagenesis, Sequencing