plasmid sequencing for validation (Plasmidsaurus)

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Plasmidsaurus plasmid sequencing for validation
Plasmid Sequencing For Validation, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+validated+plasmids/Plasmid/pmc10369909-199-11-15
Average 99 stars, based on 1 article reviews

plasmid sequencing for validation - by Bioz Stars, 2026-11

99/100 stars

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Sequencing:

Article Title: Engineered cells that sense and respond to interleukin-23 with neutralizing therapy for autoimmunity
Article Snippet: DNA fragments were purchased from Twist, and PCR primers from IDT. .. All sequences verified through whole-plasmid sequencing through Primordium or Plasmidsaurus. .. The HEK293FT cell line was purchased from ThermoFisher (R70007), and the HEK-Blue IL-23 cell line was purchased from InvivoGen (hkb-il23).

Article Title: Mobile genetic elements drive a plasmid fusion and deletion lifecycle shaping evolution and antimicrobial resistance.
Article Snippet: .. Plasmid architectures were determined by long-read sequencing performed by Plasmidsaurus (San Francisco, CA, USA) using Oxford Nanopore Technologies. ..

Article Title: Insights into the Mechanism of Allele Conversion using a Fluorescent Reporter-Based Compound Heterozygous Allele Conversion Reporter (CHACR) Cell Line Model.
Article Snippet: Illumina NGS was conducted using Genewiz 532 Amplicon-EZ service. .. Long read ONT sequencing was conducted using Plasmidsaurus 533 Premium PCR Sequencing service. ..

Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
Article Snippet: .. The correct sequence of all constructs was confirmed by whole-plasmid sequencing (Plasmidsaurus, South San Francisco, USA) ( ). .. All primers were from IDT (Integrated DNA Technologies, Coralville, USA).

Plasmid Preparation:

BAC Assay:

Article Title: Zika virus produces two isoforms of the NS2A protein that play mutually exclusive roles in viral replication and virion assembly.
Article Snippet: .. Resulting ZIKVPR cDNA BAC were purified using the QIAGEN Large-Construct kit (Qiagen), and sequences were verified by Oxford Nanopore sequencing (Plasmidsaurus). ..

Purification:

Generated:

Article Title: Synthetic reconstitution of planar polarity initiation reveals principles of symmetry breaking across scales.
Article Snippet: PiggyBac- E- cadherinmScarletI cloned from mouse E- Cadherin- GFP (a gift from Alpha Yap, University of Queensland, Addgene #67937) (58). .. All plasmids generated were verified with Plasmidsaurus Nanopore sequencing. ..

Nanopore Sequencing:

other:

Article Title: Fourteen complete genomes of nematode-mutualistic Xenorhabdus bacteria reveal their high polylysogeny.
Article Snippet: Samples were outsourced for sequencing (Plasmid saurus, USA) using Oxford Nanopore tagmentation amplification-free v14 chemistry (SDK-RAD114) libraries and R10 flow cells, with these Dorado base calling parameters: dna_r10.4.1_e8.2_400bps_sup@v4.3.0, with default Q10 quality filtering.

Polymerase Chain Reaction:

Mutagenesis:

Article Title: Small proteins CrgA and Rv0207c modulate the essential mycolic acid transporter MmpL3.
Article Snippet: .. The resulting mutant constructs were verified by whole-plasmid 388 DNA sequencing with the Plasmidsaurus platform. ..

Review