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starr seq plasmid sequence  (Addgene inc)


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    Structured Review

    Addgene inc starr seq plasmid sequence
    Starr Seq Plasmid Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence+validated+plasmids/STARR-seq+luciferase+validation+vector_ORI_neg%2Econt+(Plasmid+%2399315)/pmc06904520-374-1-11
    Average 93 stars, based on 4 article reviews
    starr seq plasmid sequence - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Cloning:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Purification:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Control:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Article Title: Identification of ALK in Thinness.
    Article Snippet: .. Enhancer (luciferase reporter) assay 5x105 SH-SY5Y cells (Sigma #94030304) were electroporated via Maxcyte STX (Cat No. GOC1) with the ‘‘luciferase reporter vector’’ (Addgene ID #99297) utilizing the ORI as a core promoter (Muerdter et al., 2018) comprising either the putative identified enhancer region spanning the ALK indel rs568057364 or a negative control (Addgene ID #99315) together with a Renilla control vector (Promega # E2261) in a ratio of 10 to 1. ..

    Sequencing:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Article Title: Widespread Long-range Cis-Regulatory Elements in the Maize Genome
    Article Snippet: .. The STARR-seq plasmid sequence and additional information can be found at Addgene, deposit number 117379 ( https://www.addgene.org/117379/ ), where it is available for purchase. .. Competing Interests Robert J Schmitz and Xiaoyu Zhang are co-founders of REquest Genomics, LLC, a company that provides epigenomics services.

    Article Title: Widespread Long-range Cis-Regulatory Elements in the Maize Genome
    Article Snippet: .. Our STARR-seq plasmid sequence and additional information can be found at Addgene, deposit number 117379 ( https://www.addgene.org/117379/ ). .. The genomic DNA input for the STARR-seq assay was a ~150 kb bacterial artifical chromosome CH201-136H12 ( https://www.maizegdb.org/data_center/bac?id=613738 ) and an ATAC-seq library derived from maize second leaves.

    Amplification:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Luciferase:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Article Title: Identification of ALK in Thinness.
    Article Snippet: .. Enhancer (luciferase reporter) assay 5x105 SH-SY5Y cells (Sigma #94030304) were electroporated via Maxcyte STX (Cat No. GOC1) with the ‘‘luciferase reporter vector’’ (Addgene ID #99297) utilizing the ORI as a core promoter (Muerdter et al., 2018) comprising either the putative identified enhancer region spanning the ALK indel rs568057364 or a negative control (Addgene ID #99315) together with a Renilla control vector (Promega # E2261) in a ratio of 10 to 1. ..

    Article Title: Glucocorticoids unmask silent non-coding genetic risk variants for common diseases
    Article Snippet: .. To validate selected STARR-seq signals with clinical significance, we conducted luciferase reporter gene assays using the STARR-seq luciferase validation vector (Addgene #9927). .. The plasmid was digested with restriction enzymes BamHI-HF® (Cat#: R3136S, NEB) and SspI-HF® (Cat#: R3132S, NEB).

    Biomarker Discovery:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Article Title: Glucocorticoids unmask silent non-coding genetic risk variants for common diseases
    Article Snippet: .. To validate selected STARR-seq signals with clinical significance, we conducted luciferase reporter gene assays using the STARR-seq luciferase validation vector (Addgene #9927). .. The plasmid was digested with restriction enzymes BamHI-HF® (Cat#: R3136S, NEB) and SspI-HF® (Cat#: R3132S, NEB).

    Plasmid Preparation:

    Article Title: Long-range interaction within the chromatin domain determines regulatory patterns in porcine skeletal muscle.
    Article Snippet: .. LUC-ENH and LUC-CON plasmids were constructed by cloning the purified enhancer and control sequence amplified from pig genomic DNA to the MfeI (NEB) and SalI (NEB) restriction enzyme sites of STARR-seq luciferase validation vector (Addgene). .. LUC-ENH, LUC-CON and plasmid without inserted fragments (named LUCMOCK) were co-transfected with pRL-TK plasmid (BNCC) into mouse C2C12 cells (ATCC) and pig IPEC-J2 cells (BNCC) and PK-15 cells (ATCC), respectively.

    Article Title: Widespread Long-range Cis-Regulatory Elements in the Maize Genome
    Article Snippet: .. The STARR-seq plasmid sequence and additional information can be found at Addgene, deposit number 117379 ( https://www.addgene.org/117379/ ), where it is available for purchase. .. Competing Interests Robert J Schmitz and Xiaoyu Zhang are co-founders of REquest Genomics, LLC, a company that provides epigenomics services.

    Article Title: Widespread Long-range Cis-Regulatory Elements in the Maize Genome
    Article Snippet: .. Our STARR-seq plasmid sequence and additional information can be found at Addgene, deposit number 117379 ( https://www.addgene.org/117379/ ). .. The genomic DNA input for the STARR-seq assay was a ~150 kb bacterial artifical chromosome CH201-136H12 ( https://www.maizegdb.org/data_center/bac?id=613738 ) and an ATAC-seq library derived from maize second leaves.

    Reporter Assay:

    Article Title: Identification of ALK in Thinness.
    Article Snippet: .. Enhancer (luciferase reporter) assay 5x105 SH-SY5Y cells (Sigma #94030304) were electroporated via Maxcyte STX (Cat No. GOC1) with the ‘‘luciferase reporter vector’’ (Addgene ID #99297) utilizing the ORI as a core promoter (Muerdter et al., 2018) comprising either the putative identified enhancer region spanning the ALK indel rs568057364 or a negative control (Addgene ID #99315) together with a Renilla control vector (Promega # E2261) in a ratio of 10 to 1. ..

    Negative Control:

    Article Title: Identification of ALK in Thinness.
    Article Snippet: .. Enhancer (luciferase reporter) assay 5x105 SH-SY5Y cells (Sigma #94030304) were electroporated via Maxcyte STX (Cat No. GOC1) with the ‘‘luciferase reporter vector’’ (Addgene ID #99297) utilizing the ORI as a core promoter (Muerdter et al., 2018) comprising either the putative identified enhancer region spanning the ALK indel rs568057364 or a negative control (Addgene ID #99315) together with a Renilla control vector (Promega # E2261) in a ratio of 10 to 1. ..

    other:

    Article Title: Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma
    Article Snippet: We modified the NFR195B clone via site-directed mutagenesis (Q5 SDM kit E0554S; NEB) to delete the indicated OCT2-binding motif (“TTTGCAT”) and to generate the patientderived mutant allele from DLBCL 14-35632 (hg38 chr8:128172453_ T>C/chr8:128172455_G>GA).



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