plasmid sequence validation (Plasmidsaurus)

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Plasmidsaurus plasmid sequence validation
Plasmid Sequence Validation, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+validated+plasmids/dna+plasmid+sequence+verification+whole/pm40825037-80-9-12
Average 86 stars, based on 1 article reviews

plasmid sequence validation - by Bioz Stars, 2026-10

86/100 stars

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Sequencing:

Article Title: Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing
Article Snippet: All plasmids used in tissue culture were prepared with Qiagen Plasmid Maxi Kit (Qiagen). .. All plasmids were sequence confirmed prior to use (Plasmidsaurus, UC Berkeley DNA Sequencing Facility). .. Lenti-X (Takara Biosciences), HEK293T, U2OS, and HeLa cells were obtained and authenticated by the UC Berkeley Cell Culture facility.

Article Title: P4-ATPases control phosphoinositide membrane asymmetry and neomycin resistance
Article Snippet: The gene was inserted using ligation-independent cloning into a vector modified from pJK369 (plasmid #73594) acquired from Addgene for the expression of Neo1. .. After DNA sequence verification (Plasmidsaurus), the S. cerevisiae strain BY4741 Δpep4::kanMX6 (Horizon Discovery) was transformed with the constructed vector using Quick and Easy Yeast Transformation Mix (Takara Bio). ..

Article Title: PPM1M, an LRRK2-counteracting, phosphoRab12-preferring phosphatase with a potential link to Parkinson's disease.
Article Snippet: DNA constructs were amplified in E. coli DH5α and purified using mini prep columns (Econospin, Fisher Scientific). .. Whole plasmid DNA sequence verification was performed by Primordium/Plasmidsaurus (https://plasmidsaurus.com). pET15b His-MST3 was a kind gift of Amir Khan (Trinity College, Dublin). pCMV5D HA-empty or HA-PPM1H, PPM1H H153D, PPM1H D288A, PPM1M, PPM1M H127D, PPM1M D235A, and PPM1M D440N were obtained from MRC PPU. pET15b His-SUMO-Rab10 Q68L and Rab12 Q101L were previously obtained or cloned. pET15b His-SUMO-PPM1M was cloned using the pET15b backbone and HAPPM1M insert via Gibson assembly. ..

Article Title: Nonequivalence of Zfp423 premature termination codons in mice.
Article Snippet: .. A single base clone was selected based on whole-7 plasmid sequence validation (Plasmidsaurus). ..

Article Title: P4-ATPases control phosphoinositide membrane asymmetry and neomycin resistance.
Article Snippet: The gene was inserted using ligation-independent cloning into a vector modified from pJK369 (plasmid #73594) acquired from Addgene for the expression of Neo1. .. After DNA sequence verification (Plasmidsaurus), the S. cerevisiae strain BY4741 Δpep4::kanMX6 (Horizon Discovery) was transformed with the constructed vector using Quick and Easy Yeast Transformation Mix (Takara Bio). .. The cells were collected and approximately 50,000 cells were seeded on 35-mm glass-bottom MatTek Dishes (P35G-1.5-14-C) and allowed to attach to the dish for 24 h. After 24 h, the cells were washed with 1 ml of 1× PBS and Hoechst stain (33342, 1:1,000) in PBS was added to the cells and incubated for 15 min at 37 °C.

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other:

Article Title: Aromatic acid metabolism in Methylobacterium extorquens reveals interplay between methylotrophic and heterotrophic pathways.
Article Snippet: Fragments for deletion vectors were constructed by inserting 500–800 bp regions of M. extorquens SLI 505 genomic DNA flanking the gene of interest into pCM433KanT via HiFi DNA Assembly MasterMix (New England Biolabs).

Article Title: Peptide Recognition Sequence Guides Catalytic Side Chain Cross-Linking of Plant Peptides by Copper-Dependent Cyclases
Article Snippet: The ArbA223150 was assembled into pET28-MBP plasmid using Gibson Assembly Master Mix (New England Biolabs).

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