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sequencing library construction  (Illumina Inc)


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    Structured Review

    Illumina Inc sequencing library construction
    Sequencing Library Construction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 13044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence+libraries+construction/TruSeq+DNA+Library+Prep/pm41275828-98-1-5
    Average 99 stars, based on 13044 article reviews
    sequencing library construction - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Multiplexing:

    Article Title: Phylogenomic species delimitation reveals distinct pace and mode of lineage evolution in Amanita jacksonii.
    Article Snippet: Amanita jacksonii s.l. represents a challenging case of species delimitation in mushroom-forming fungi, where traditional morphological characters and standard molecular markers have proven insufficient for clear taxonomic resolution.. Using exon-targeted sequencing of over 100 genetic loci from 63 specimens across North America, we provide robust phylogenomic evidence supporting seven distinct species within the closely related group.. Our analyses recovered 30,342 high-quality variants and 172 loci averaging 2.5 Kb in length, enabling comprehensive genetic structure and species delimitation analyses.

    Construct:

    Article Title: Multi-omics analysis identifies key microbial taxa and host genes controlling abdominal fat deposition in chickens
    Article Snippet: .. Genomic libraries were constructed using the TruSeq Nano DNA Library Prep Kit (Illumina, San Diego, CA, USA), and high-throughput paired-end sequencing was performed on the Illumina NovaSeq X Plus platform. ..

    Article Title: Dietary iron overload exacerbates intestinal damage induced by Eimeria tenella infection in broilers via impaired barrier integrity and gut microbiota dysbiosis
    Article Snippet: The quantity and quality of the DNA were assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. .. Metagenomic shotgun sequencing libraries (insert size 400 bp) were constructed using the Illumina TruSeq Nano DNA LT Library Preparation Kit and sequenced on the Illumina HiSeq X-ten platform (Illumina, USA) employing a PE150 strategy at Personal Biotechnology Co., Ltd. (Shanghai, China). ..

    Article Title: The Role of Gut Microbiota and Their Derived Metabolites in Chemotherapy-Induced Nausea and Vomiting in Ovarian Cancer.
    Article Snippet: The quantity and quality of the extracted DNA were assessed using a NanoDrop ND- 1000 spectrophotometer (Thermo Fisher Scientific, USA) and agarose gel electrophoresis, respectively. .. Extracted DNA was used to construct metagenomic shotgun sequencing libraries with an insert size of approximately 400 bp using the Illumina TruSeq Nano DNA LT Library Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq X- ten platform (Illumina, USA) using 150- bp paired- end (PE150) sequencing at Suzhou PANOMIX Biomedical Tech Co. Ltd.

    Article Title: The Role of Gut Microbiota and Their Derived Metabolites in Chemotherapy‐Induced Nausea and Vomiting in Ovarian Cancer
    Article Snippet: The quantity and quality of the extracted DNA were assessed using a NanoDrop ND‐1000 spectrophotometer (Thermo Fisher Scientific, USA) and agarose gel electrophoresis, respectively. .. Extracted DNA was used to construct metagenomic shotgun sequencing libraries with an insert size of approximately 400 bp using the Illumina TruSeq Nano DNA LT Library Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq X‐ten platform (Illumina, USA) using 150‐bp paired‐end (PE150) sequencing at Suzhou PANOMIX Biomedical Tech Co. Ltd.

    Article Title: Chromosome-level de novo genome assembly and whole-genome resequencing provide insights into genome evolution and sex determination of sleepy cod Oxyeleotris lineolata (Steindachner 1867).
    Article Snippet: .. Libraries were constructed according to the standard TruSeq DNA library preparation procedure provided by Illumina Inc., and paired-end sequencing with 150-bp read lengths was conducted on the Illumina HiSeqTM 2000 platform. ..

    Sequencing:

    Article Title: Multi-omics analysis identifies key microbial taxa and host genes controlling abdominal fat deposition in chickens
    Article Snippet: .. Genomic libraries were constructed using the TruSeq Nano DNA Library Prep Kit (Illumina, San Diego, CA, USA), and high-throughput paired-end sequencing was performed on the Illumina NovaSeq X Plus platform. ..

    Article Title: Chromosome-level de novo genome assembly and whole-genome resequencing provide insights into genome evolution and sex determination of sleepy cod Oxyeleotris lineolata (Steindachner 1867).
    Article Snippet: .. Libraries were constructed according to the standard TruSeq DNA library preparation procedure provided by Illumina Inc., and paired-end sequencing with 150-bp read lengths was conducted on the Illumina HiSeqTM 2000 platform. ..

    Shotgun Sequencing:

    Article Title: Dietary iron overload exacerbates intestinal damage induced by Eimeria tenella infection in broilers via impaired barrier integrity and gut microbiota dysbiosis
    Article Snippet: The quantity and quality of the DNA were assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. .. Metagenomic shotgun sequencing libraries (insert size 400 bp) were constructed using the Illumina TruSeq Nano DNA LT Library Preparation Kit and sequenced on the Illumina HiSeq X-ten platform (Illumina, USA) employing a PE150 strategy at Personal Biotechnology Co., Ltd. (Shanghai, China). ..

    Article Title: The Role of Gut Microbiota and Their Derived Metabolites in Chemotherapy-Induced Nausea and Vomiting in Ovarian Cancer.
    Article Snippet: The quantity and quality of the extracted DNA were assessed using a NanoDrop ND- 1000 spectrophotometer (Thermo Fisher Scientific, USA) and agarose gel electrophoresis, respectively. .. Extracted DNA was used to construct metagenomic shotgun sequencing libraries with an insert size of approximately 400 bp using the Illumina TruSeq Nano DNA LT Library Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq X- ten platform (Illumina, USA) using 150- bp paired- end (PE150) sequencing at Suzhou PANOMIX Biomedical Tech Co. Ltd.

    Article Title: The Role of Gut Microbiota and Their Derived Metabolites in Chemotherapy‐Induced Nausea and Vomiting in Ovarian Cancer
    Article Snippet: The quantity and quality of the extracted DNA were assessed using a NanoDrop ND‐1000 spectrophotometer (Thermo Fisher Scientific, USA) and agarose gel electrophoresis, respectively. .. Extracted DNA was used to construct metagenomic shotgun sequencing libraries with an insert size of approximately 400 bp using the Illumina TruSeq Nano DNA LT Library Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq X‐ten platform (Illumina, USA) using 150‐bp paired‐end (PE150) sequencing at Suzhou PANOMIX Biomedical Tech Co. Ltd.

    DNA Library Preparation:

    Article Title: Chromosome-level de novo genome assembly and whole-genome resequencing provide insights into genome evolution and sex determination of sleepy cod Oxyeleotris lineolata (Steindachner 1867).
    Article Snippet: .. Libraries were constructed according to the standard TruSeq DNA library preparation procedure provided by Illumina Inc., and paired-end sequencing with 150-bp read lengths was conducted on the Illumina HiSeqTM 2000 platform. ..

    Polymerase Chain Reaction:

    Article Title: Exosome-mediated decay of unstable long extended precursors of human telomerase RNA is dependent on 5′-cap trimethylation
    Article Snippet: PCR amplification was carried out using universal RT primer (sequence: 5′-CTACGTAACGATTGATGGTGCCTACAG-3′) and hTR_L3 (sequence: 5′-CTCTGTCAGCCGCGGGTCTCT C-3′) with Phusion high-fidelity DNA polymerase (NEB M0530). .. PCR products were subjected to QIAquick PCR purification columns (Qiagen) for library preparation using the TruSeq Nano DNA LT library preparation kit (Illumina). ..

    Purification:

    Article Title: Exosome-mediated decay of unstable long extended precursors of human telomerase RNA is dependent on 5′-cap trimethylation
    Article Snippet: PCR amplification was carried out using universal RT primer (sequence: 5′-CTACGTAACGATTGATGGTGCCTACAG-3′) and hTR_L3 (sequence: 5′-CTCTGTCAGCCGCGGGTCTCT C-3′) with Phusion high-fidelity DNA polymerase (NEB M0530). .. PCR products were subjected to QIAquick PCR purification columns (Qiagen) for library preparation using the TruSeq Nano DNA LT library preparation kit (Illumina). ..



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