multiple sequence alignment and identity/similarity matrix  (MacVector inc)

Journal: Scientific Reports

Article Title: Genomic insights into a novel species, Dyella thailandensis sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil associated with leaf compost

doi: 10.1038/s41598-025-33717-w

Figure Lengend Snippet: Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences illustrating position of strain KULCS107 T relative to all closely related species of the genera Dyella and Frateuria , with Xanthomonas campestris ATCC 33913 T as the outgroup. Bootstrap values (based on 1,000 replications) greater than 50% are shown at the nodes. The analysis was based on an alignment of 1,545 nucleotide positions. GenBank accession numbers are in parentheses. Bar, 0.02 substitutions per nucleotide site.

Article Snippet: In the resulting trees, the strain formed a distinct phylogenetic lineage with its closest relative, D. ginsengisoli Gsoil 3046 T . Since the 16S rRNA gene sequence identity between these 2 strains exceeds the 98.65% threshold commonly used for species delineation , further genome-based analyses were performed to clarify the taxonomic status of strain KULCS107 T . Fig. 1 Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences illustrating position of strain KULCS107 T relative to all closely related species of the genera Dyella and Frateuria , with Xanthomonas campestris ATCC 33913 T as the outgroup.

Techniques: