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megalign  (Lasergen Inc)

 
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    Lasergen Inc megalign
    ( A ) Neighbor-joining phylogenic tree of amino acid sequences encoding Kir channel subunits in insects and humans. Geneious Prime 2023.2.2 ( www.geneious.com ) was used to construct the tree. The Genbank® accession numbers of the Kir sequences are shown in parentheses. Underlines denote Apis mellifera Kir channel subunits. The abbreviations of the species are as follows: Aag: Aedes aegypti , Agam: Anopheles gambiae , Agly: Aphis glycines , Amel: Apis mellifera , Apisu: Acyrthosiphon pisum , Clec: Cimex lectularius , Dmel: Drosophila melanogaster , Hsap: Homo sapiens , Nlug: Nilaparvata lugens . ( B ) Evaluation of expression of AmKir channel isotypes in honeybee tissues and life stage. Tissue-specific expression of the AmKir channel isotype was determined using RT-PCR. All honeybee tissue samples underwent identical preparation steps, from dissection to gel electrophoresis. The sizes of the ladder marker next to the fist blot is the same for all the other blots, and are indicated in base pairs (bp). Abbreviations: LE: legs, LA: larvae, HE: heads, BR: brains, GAG: ganglia, GU: guts, MU: muscles, ANT: antennae ( C ) Sequence alignments of the three current-generating AmKir channel isoforms were performed using <t>MegAlign</t> from Lasergen. The blue squares indicate the hydropathy estimation of the transmembrane regions. The red square represents the estimated region of the selectivity filter. The black squares indicate arginine-glutamate salt bridge interactions forming the PIP 2 -binding site in the cytoplasmic domain.
    Megalign, supplied by Lasergen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence+analysis+software+megalign+program/pmc10991380-115-32-34?v=Lasergen+Inc
    Average 90 stars, based on 1 article reviews
    megalign - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Cloning, functional expression, and pharmacological characterization of inwardly rectifying potassium channels (Kir) from Apis mellifera"

    Article Title: Cloning, functional expression, and pharmacological characterization of inwardly rectifying potassium channels (Kir) from Apis mellifera

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-58234-0

    ( A ) Neighbor-joining phylogenic tree of amino acid sequences encoding Kir channel subunits in insects and humans. Geneious Prime 2023.2.2 ( www.geneious.com ) was used to construct the tree. The Genbank® accession numbers of the Kir sequences are shown in parentheses. Underlines denote Apis mellifera Kir channel subunits. The abbreviations of the species are as follows: Aag: Aedes aegypti , Agam: Anopheles gambiae , Agly: Aphis glycines , Amel: Apis mellifera , Apisu: Acyrthosiphon pisum , Clec: Cimex lectularius , Dmel: Drosophila melanogaster , Hsap: Homo sapiens , Nlug: Nilaparvata lugens . ( B ) Evaluation of expression of AmKir channel isotypes in honeybee tissues and life stage. Tissue-specific expression of the AmKir channel isotype was determined using RT-PCR. All honeybee tissue samples underwent identical preparation steps, from dissection to gel electrophoresis. The sizes of the ladder marker next to the fist blot is the same for all the other blots, and are indicated in base pairs (bp). Abbreviations: LE: legs, LA: larvae, HE: heads, BR: brains, GAG: ganglia, GU: guts, MU: muscles, ANT: antennae ( C ) Sequence alignments of the three current-generating AmKir channel isoforms were performed using MegAlign from Lasergen. The blue squares indicate the hydropathy estimation of the transmembrane regions. The red square represents the estimated region of the selectivity filter. The black squares indicate arginine-glutamate salt bridge interactions forming the PIP 2 -binding site in the cytoplasmic domain.
    Figure Legend Snippet: ( A ) Neighbor-joining phylogenic tree of amino acid sequences encoding Kir channel subunits in insects and humans. Geneious Prime 2023.2.2 ( www.geneious.com ) was used to construct the tree. The Genbank® accession numbers of the Kir sequences are shown in parentheses. Underlines denote Apis mellifera Kir channel subunits. The abbreviations of the species are as follows: Aag: Aedes aegypti , Agam: Anopheles gambiae , Agly: Aphis glycines , Amel: Apis mellifera , Apisu: Acyrthosiphon pisum , Clec: Cimex lectularius , Dmel: Drosophila melanogaster , Hsap: Homo sapiens , Nlug: Nilaparvata lugens . ( B ) Evaluation of expression of AmKir channel isotypes in honeybee tissues and life stage. Tissue-specific expression of the AmKir channel isotype was determined using RT-PCR. All honeybee tissue samples underwent identical preparation steps, from dissection to gel electrophoresis. The sizes of the ladder marker next to the fist blot is the same for all the other blots, and are indicated in base pairs (bp). Abbreviations: LE: legs, LA: larvae, HE: heads, BR: brains, GAG: ganglia, GU: guts, MU: muscles, ANT: antennae ( C ) Sequence alignments of the three current-generating AmKir channel isoforms were performed using MegAlign from Lasergen. The blue squares indicate the hydropathy estimation of the transmembrane regions. The red square represents the estimated region of the selectivity filter. The black squares indicate arginine-glutamate salt bridge interactions forming the PIP 2 -binding site in the cytoplasmic domain.

    Techniques Used: Construct, Expressing, Reverse Transcription Polymerase Chain Reaction, Dissection, Nucleic Acid Electrophoresis, Marker, Muscles, Sequencing, Binding Assay



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