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Addgene inc sep-glua2
Sep Glua2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sep-glua2/sep+glua1/pmc11194458-133-2-8
Average 90 stars, based on 1 article reviews
sep-glua2 - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

other:

Article Title: Fluorescence lifetime imaging of AMPA receptor endocytosis in living neurons: effects of Aβ and PP1
Article Snippet: SEP-GluA1 and SEP-GluA2 were gifts from Roberto Malinow (Addgene plasmids # 24000 and # 24002; RRID:Addgene_24000 , RRID:Addgene_24002 , respectively).

Article Title: The tetraspanin TSPAN5 regulates AMPARs exocytosis by interacting with the AP-4 complex
Article Snippet: Rab4-GFP, Rab7-GFP and Rab11-GFP are kind gifts from Prof G. Schiavo. pCl-SEP-GluA2 was obtained from Addgene #24001 ( ).

Article Title: AMPA Receptor Number and Dynamics on a Synapse-to-Synapse Basis During Maintenance of Chemical LTP
Article Snippet: Chaoyi Jin1,2, Sung-Soo Jang4, Pinghua Ge2,3, Hee Jung Chung4, Paul R. Selvin1,2,3,* 1Center for Biophysics and Quantitative Biology; 2Center for the Physics of Living Cells, 3Physics Dept.. 4Department of Molecular and Integrative Physiology, 5Neuroscience Program, University of Illinois at Urbana–Champaign, Urbana, IL, United States, 61801.. Corresponding author: Paul R. Selvin Email: selvin@illinois.edu Author Contributions: Chaoyi Jin: conceptualization, taking fluorescence data and analyze, investigation, methodology, writing manuscript Sung-Soo Jang: taking biochemistry data and analyze, Electrophysiological recording and analysis, methodology, investigation Pinghua Ge: methodology, investigation Hee Jung Chung: conceptualization, investigation, writing, funding, resources Paul Selvin: conceptualization, investigation, writing, funding, resources, project administration Competing Interest Statement: No competing interests declared.

Article Title: Rabphilin-3A Drives Structural Modifications of Dendritic Spines Induced by Long-Term Potentiation
Article Snippet: The hSyn-RFP-WPRE, hSyn-RFPRph3A-WPRE, and CMV-SEP-GluA1 plasmids were purchased from Addgene.

Article Title: Synaptic activity-dependent changes in the hippocampal palmitoylome.
Article Snippet: Transfection of primary hippocampal cultures and HEK293T cells Primary hippocampal cultures were transfected at 9 to 10 days in vitro (DIV) using Lipofectamine 2000 (Invitrogen) according to the manufacturer ’s recommendations.

Article Title: Synaptic Activity-Dependent Changes in the Hippocampal Palmitoylome
Article Snippet: The SEP-GluA1 construct was generated by Dr Roberto Malinow (University of California, San Diego) and obtained from Addgene (Watertown, MA: Plasmid #24000).

Clone Assay:

Article Title: Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation
Article Snippet: .. The fragments were assembled together with Gibson Assembly ( ) and cloned into pLenti hSynI vector (HT075 from ( )). pTL028 (Addgene 192520): Cre-on AAV expression vector of HaloTag fused to the N terminus of GluA1, driven by human synapsin 1 gene (hSynI) promoter. .. The open reading frame of pTL024 was flipped and cloned into a pAAV_hSyn-DIO vector (Addgene 107704).

Bioprocessing:

Article Title: Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation
Article Snippet: .. The fragments were assembled together with Gibson Assembly ( ) and cloned into pLenti hSynI vector (HT075 from ( )). pTL028 (Addgene 192520): Cre-on AAV expression vector of HaloTag fused to the N terminus of GluA1, driven by human synapsin 1 gene (hSynI) promoter. .. The open reading frame of pTL024 was flipped and cloned into a pAAV_hSyn-DIO vector (Addgene 107704).

Expressing:

Article Title: Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation
Article Snippet: .. The fragments were assembled together with Gibson Assembly ( ) and cloned into pLenti hSynI vector (HT075 from ( )). pTL028 (Addgene 192520): Cre-on AAV expression vector of HaloTag fused to the N terminus of GluA1, driven by human synapsin 1 gene (hSynI) promoter. .. The open reading frame of pTL024 was flipped and cloned into a pAAV_hSyn-DIO vector (Addgene 107704).

Plasmid Preparation:

Article Title: Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation
Article Snippet: .. The fragments were assembled together with Gibson Assembly ( ) and cloned into pLenti hSynI vector (HT075 from ( )). pTL028 (Addgene 192520): Cre-on AAV expression vector of HaloTag fused to the N terminus of GluA1, driven by human synapsin 1 gene (hSynI) promoter. .. The open reading frame of pTL024 was flipped and cloned into a pAAV_hSyn-DIO vector (Addgene 107704).



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