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sep glua2 q (Addgene inc)

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Addgene inc sep glua2 q
Sep Glua2 Q, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sep-glua2/pCF823_EFS-Cas9-PuroR_lenti+(Plasmid+%23138317)/pm37914930-571-1-2
Average 92 stars, based on 1 article reviews

sep glua2 q - by Bioz Stars, 2026-10

92/100 stars

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Plasmid Preparation:

Article Title: Glioma synapses recruit mechanisms of adaptive plasticity.
Article Snippet: .. For PSD95-PURO, Addgene plasmid EFS-Cas9-Puro (#138317) was digested with AgeI, BamHI, and EcoRV; the 6.7 kb lentiviral backbone was isolated via gel extraction. ..

Article Title: Glioma synapses recruit mechanisms of adaptive plasticity
Article Snippet: .. For SEP–GluA2(Q)–TagBFP, Addgene plasmid EFS-Cas9-Puro (#138317) was digested with AgeI, MluI, and EcoRV; the 6 kb lentiviral backbone was isolated via gel extraction. .. The SEP fragment with Gibson overhangs was amplified from Addgene plasmid pCI-SEP-GluR2(Q) (#24002) with primers: 5′-AACGGGTTTGCCGCCAGAACACAGGACCGGTGCCACCATGCAAAAGATTATGCATATTTC-3′ and 5′-CCCCCTATCTGTATGCTGTTGCTAGCTTTGTATAGTTCATC-3′.

Isolation:

Gel Extraction:

other:

Article Title: Glioma synapses recruit mechanisms of adaptive plasticity.
Article Snippet: A tunable Ti:Sapphire laser (Spectra Physics Mai Tai DeepSee) provided the excitation light (920 nm) for both tdTomato and GCaMP6s.