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Johns Hopkins HealthCare sep–glua2 mice
Sep–Glua2 Mice, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sep-glua2/sep+glua2/pm37169928-180-0-8
Average 90 stars, based on 1 article reviews
sep–glua2 mice - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Cross-modality supervised image restoration enables nanoscale tracking of synaptic plasticity in living mice
Article Snippet: SEP-GluA2 mice were made in collaboration with the Johns Hopkins University School of Medicine Transgenics core.

Article Title: p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer's disease.
Article Snippet: Plasmids expressing Myc-GluA1 or Myc-GluA2 were kindly provided by Dr. Alex L. Kolodkin from The Johns Hopkins School of Medicine.

Article Title: Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation
Article Snippet: The myc-GluA2 plasmid was provided by the Richard Huganir lab at Johns Hopkins University ( 10, 12, 13 ).

Article Title: Cross-modality supervised image restoration enables nanoscale tracking of synaptic plasticity in living mice.
Article Snippet: SEP–GluA2 mice were made in collaboration with the Johns Hopkins University School of Medicine Transgenics core, on a C57/BL6 background.

Article Title: EPSILON: a method for pulse-chase labeling to probe synaptic AMPAR exocytosis during memory formation.
Article Snippet: A tool to map changes in synaptic strength during a defined time window could provide powerful insights into the mechanisms of learning and memory.. Here we developed a technique, Extracellular Protein Surface Labeling in Neurons (EPSILON), to map α-amino-3-hydroxy5-methyl-4-isoxazolepropionic acid receptor (AMPAR) exocytosis in vivo by sequential pulse-chase labeling of surface AMPARs with membrane-impermeable dyes.. This approach yields synaptic-resolution maps of AMPAR exocytosis, a proxy for synaptic potentiation, in genetically targeted neurons during memory formation.

Article Title: Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation
Article Snippet: The myc-GluA2 plasmid was provided by the Richard Huganir lab at Johns Hopkins University , , .

Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sodium bicarbonate solution Sigma Cat# S8761 Tetrodotoxine citrate Tocris Cat# 1069 Triton X-100 Sigma-Aldrich Cat# X100-500ML Recombinant DNA pNeuroD-IRES-CRE-GFP Barnat et al, 2017 N/A pCALNL-GFP Addgene Cat# 13770; RRID: Addgene_13770 pCAGGS-NLS-GFP Provided by L. Nguyen, University of Liège, GIGA N/A pCAG-tdTomato Addgene Cat# 83029; RRID: Addgene_83029 GFP-UtrCH Addgene Cat# 26737; RRID: Addgene_26737 pCAG-cofilin-S3A Addgene Cat# 78296; RRID: Addgene_78296 pCAG-cofilin-S3D Addgene Cat# 78297; RRID: Addgene_78297 pCAG-cofilin-S3A-HA This paper N/A p-ORANGE GFP-Actb-KI Provided by H. MacGillavry, Utrecht University N/A pCAG-SEP-GluA1 Provided by R.Huganir, Johns Hopkins University, Baltimore N/A Experimental models: Organisms/strains Mouse: SWISS-CD1 Janvier Lab N/A Mouse: Hdhlox/lox Dragatsis et al., 2000 N/A Mouse: NEXCRE/+ Goebbels et al., 2006 N/A Software and algorithms Clampfit 11.1 Molecular devices https://www.moleculardevices.com/ products/axon-patch-clamp-system/ acquisition-and-analysis-software/ pclamp-software-suite EthoVision XT 14/15 Noldus https://www.noldus.com/ethovision-xt Image J/Fiji NIH https://imagej.nih.gov/ij/download.html Graph pad Prism 8.0.2 Graphpad software https://www.graphpad.com NeuronStudio ISMMS OBS Studio OBS Project https://obsproject.com/es/download PatchMaster 2x90.3 HEKA https://www.heka.com/downloads/ downloads_main.html#down_ patchmaster_next Signal 5.04 Cambridge Electronic Design http://ced.co.uk/downloads/latestsoftware

Transgenic Assay:

Article Title: p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer’s disease
Article Snippet: 5×FAD male mice were from the Jackson Laboratory (Bar Harbor, ME). .. Heterozygous GluA1-mutated transgenic mice in which Ser845 of GluA1 is mutated to Ala (S845A) (gifts from Dr. Hey-Kyoung Lee, the Johns Hopkins School of Medicine, USA) were breed to obtain homozygous ones. ..



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