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surface  (Cytiva Europe)


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    Structured Review

    Cytiva Europe surface
    Surface, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 2088 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sensor+surface/Sensor+Chip+CM5/us12643957-1211-2-8
    Average 98 stars, based on 2088 article reviews
    surface - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    SPR Assay:

    Article Title: Direct targeting of LYN kinase by fluphenazine triggers ferroptosis to suppress lung adenocarcinoma.
    Article Snippet: Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, China College of Pharmaceutical Sciences, Soochow University, Suzhou, China Institute of Respiratory Diseases, Soochow University, Suzhou, China Suzhou Key Laboratory for Respiratory Diseases, Suzhou, China Cancer Institute, Suzhou Medical College, Soochow University, Suzhou, China

    Recombinant:

    Article Title: Direct targeting of LYN kinase by fluphenazine triggers ferroptosis to suppress lung adenocarcinoma.
    Article Snippet: Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, China College of Pharmaceutical Sciences, Soochow University, Suzhou, China Institute of Respiratory Diseases, Soochow University, Suzhou, China Suzhou Key Laboratory for Respiratory Diseases, Suzhou, China Cancer Institute, Suzhou Medical College, Soochow University, Suzhou, China

    Article Title: Paeoniflorigenone triggers TRIM28 phosphorylation-dependent ferroptosis and immune activation to suppress intrahepatic cholangiocarcinoma.
    Article Snippet: SPR binding assays were performed on a Biacore 1 K system (Cytiva) at 25 ◦C. .. Recombinant TRIM28 protein (ligand) was immobilized onto the active channel (Channel 2) of a CM5 sensor chip (Cytiva) via amine coupling, using 50 μg/mL protein in 10 mM sodium acetate buffer (pH 4.0) at a flow rate of 10 μL/min for 420 s. The reference channel (Channel 1) was subjected to identical activation and blocking procedures without protein immobilization for background subtraction. ..

    Article Title: A small molecule inhibits Ebola virus entry through glycoprotein stabilization.
    Article Snippet: Surface plasmon resonance (SPR) was used to evaluate the binding of EBOV GP-targeting compounds to the EBOV GP ectodomain using a Biacore S200 system (Cytiva). .. Recombinant EBOVGP ectodomain was immobilized on a CM5 sensor chip (Cytiva) via chemical crosslinking. ..

    Article Title: Hijacking of host PCNA by circovirus replication-associated protein to recruit POLD1 drives viral DNA replication and is inhibited by R428
    Article Snippet: Binding affinity and kinetics evaluation of small molecule compounds with target proteins were performed using a Biacore 1K system (Cytiva). .. Recombinant PCNA and its mutant form (mPCNA) were immobilized onto a Series S Sensor Chip CM5 (BR-1005–30, Cytiva) via amine-coupling chemistry under standard conditions (20 μg/mL in 10 mM sodium acetate buffer, pH 4.5) at a continuous flow rate of 10 μL/min for 10 minutes at 25°C. .. Analyte R428 was serially diluted in running buffer (1 × PBS-P + , Cytiva) containing 5% (v/v) DMSO.

    Covalent Coupling:

    Article Title: Paeoniflorigenone triggers TRIM28 phosphorylation-dependent ferroptosis and immune activation to suppress intrahepatic cholangiocarcinoma.
    Article Snippet: SPR binding assays were performed on a Biacore 1 K system (Cytiva) at 25 ◦C. .. Recombinant TRIM28 protein (ligand) was immobilized onto the active channel (Channel 2) of a CM5 sensor chip (Cytiva) via amine coupling, using 50 μg/mL protein in 10 mM sodium acetate buffer (pH 4.0) at a flow rate of 10 μL/min for 420 s. The reference channel (Channel 1) was subjected to identical activation and blocking procedures without protein immobilization for background subtraction. ..

    Article Title: Targeting mutant NPM1-expressing AML with T cell receptor-like antibodies
    Article Snippet: Antibody–protein interaction was performed on a Biacore 8K (GE & Cytiva). .. The pNPM1-HLA-A2 complex was immobilized at low density on a sensor chip CM5 (#BR100399, Cytiva) via amine-coupling chemistry. ..

    Article Title: Replication protein A1 from L. donovani interacts with RECQb via the C-terminal OB fold and stimulates its helicase activity on branched substrates.
    Article Snippet: doi:10.1111/febs.70742 RecQ helicases are ubiquitous genome maintenance proteins that can resolve complex DNA structures during replication, recombination, and repair.. Eukaryotic RecQ helicases are known to interact with replication protein A (RPA), leading to the stimulation of their unwinding activity.. In higher eukaryotes, this interaction is mediated through the acidic residues of RecQ helicases and the N-terminal OB fold (DBD-F) of RPA70.

    Article Title: Hijacking of host PCNA by circovirus replication-associated protein to recruit POLD1 drives viral DNA replication and is inhibited by R428
    Article Snippet: Binding affinity and kinetics evaluation of small molecule compounds with target proteins were performed using a Biacore 1K system (Cytiva). .. Recombinant PCNA and its mutant form (mPCNA) were immobilized onto a Series S Sensor Chip CM5 (BR-1005–30, Cytiva) via amine-coupling chemistry under standard conditions (20 μg/mL in 10 mM sodium acetate buffer, pH 4.5) at a continuous flow rate of 10 μL/min for 10 minutes at 25°C. .. Analyte R428 was serially diluted in running buffer (1 × PBS-P + , Cytiva) containing 5% (v/v) DMSO.

    Activation Assay:

    Article Title: Paeoniflorigenone triggers TRIM28 phosphorylation-dependent ferroptosis and immune activation to suppress intrahepatic cholangiocarcinoma.
    Article Snippet: SPR binding assays were performed on a Biacore 1 K system (Cytiva) at 25 ◦C. .. Recombinant TRIM28 protein (ligand) was immobilized onto the active channel (Channel 2) of a CM5 sensor chip (Cytiva) via amine coupling, using 50 μg/mL protein in 10 mM sodium acetate buffer (pH 4.0) at a flow rate of 10 μL/min for 420 s. The reference channel (Channel 1) was subjected to identical activation and blocking procedures without protein immobilization for background subtraction. ..

    Blocking Assay:

    Article Title: Paeoniflorigenone triggers TRIM28 phosphorylation-dependent ferroptosis and immune activation to suppress intrahepatic cholangiocarcinoma.
    Article Snippet: SPR binding assays were performed on a Biacore 1 K system (Cytiva) at 25 ◦C. .. Recombinant TRIM28 protein (ligand) was immobilized onto the active channel (Channel 2) of a CM5 sensor chip (Cytiva) via amine coupling, using 50 μg/mL protein in 10 mM sodium acetate buffer (pH 4.0) at a flow rate of 10 μL/min for 420 s. The reference channel (Channel 1) was subjected to identical activation and blocking procedures without protein immobilization for background subtraction. ..

    Reference Subtraction:

    Article Title: Paeoniflorigenone triggers TRIM28 phosphorylation-dependent ferroptosis and immune activation to suppress intrahepatic cholangiocarcinoma.
    Article Snippet: SPR binding assays were performed on a Biacore 1 K system (Cytiva) at 25 ◦C. .. Recombinant TRIM28 protein (ligand) was immobilized onto the active channel (Channel 2) of a CM5 sensor chip (Cytiva) via amine coupling, using 50 μg/mL protein in 10 mM sodium acetate buffer (pH 4.0) at a flow rate of 10 μL/min for 420 s. The reference channel (Channel 1) was subjected to identical activation and blocking procedures without protein immobilization for background subtraction. ..

    Concentration Assay:

    Article Title: Replication protein A1 from L. donovani interacts with RECQb via the C-terminal OB fold and stimulates its helicase activity on branched substrates.
    Article Snippet: doi:10.1111/febs.70742 RecQ helicases are ubiquitous genome maintenance proteins that can resolve complex DNA structures during replication, recombination, and repair.. Eukaryotic RecQ helicases are known to interact with replication protein A (RPA), leading to the stimulation of their unwinding activity.. In higher eukaryotes, this interaction is mediated through the acidic residues of RecQ helicases and the N-terminal OB fold (DBD-F) of RPA70.

    Mutagenesis:

    Article Title: Hijacking of host PCNA by circovirus replication-associated protein to recruit POLD1 drives viral DNA replication and is inhibited by R428
    Article Snippet: Binding affinity and kinetics evaluation of small molecule compounds with target proteins were performed using a Biacore 1K system (Cytiva). .. Recombinant PCNA and its mutant form (mPCNA) were immobilized onto a Series S Sensor Chip CM5 (BR-1005–30, Cytiva) via amine-coupling chemistry under standard conditions (20 μg/mL in 10 mM sodium acetate buffer, pH 4.5) at a continuous flow rate of 10 μL/min for 10 minutes at 25°C. .. Analyte R428 was serially diluted in running buffer (1 × PBS-P + , Cytiva) containing 5% (v/v) DMSO.



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