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self complementary aav scaav vectors  (Addgene inc)


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    Structured Review

    Addgene inc self complementary aav scaav vectors
    Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in <t>scAAV</t> (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).
    Self Complementary Aav Scaav Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/self+complementary+aav+vector/scAAV+GFP+(Plasmid+%2321893)/pmc11407971-233-1-11
    Average 92 stars, based on 7 article reviews
    self complementary aav scaav vectors - by Bioz Stars, 2026-11
    92/100 stars

    Images

    1) Product Images from "Simple and highly specific targeting of resident microglia with adeno-associated virus"

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

    Journal: iScience

    doi: 10.1016/j.isci.2024.110706

    Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).
    Figure Legend Snippet: Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

    Techniques Used: Virus, Injection, Marker, Expressing, Transduction


    Figure Legend Snippet:

    Techniques Used: Recombinant, Software

    Related Articles

    Bioprocessing:

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus
    Article Snippet: .. The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter. ..

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus
    Article Snippet: .. The AAV vectors, scAAV-hIBA1a-GFP and pAAV-hIBA1a-GFP-miR124T , are available from Addgene (#214146 and #214147). ..

    Clone Assay:

    Article Title: Inhibitors of creatine transport and uses thereof
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BgIII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Article Title: Treatment and diagnosis of colon cancer
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BglII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Article Title: Treatment and diagnosis of colon cancer
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BglII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Genomic Sequencing:

    Article Title: Inhibitors of creatine transport and uses thereof
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BgIII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Article Title: Treatment and diagnosis of colon cancer
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BglII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Article Title: Treatment and diagnosis of colon cancer
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BglII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Plasmid Preparation:

    Article Title: Inhibitors of creatine transport and uses thereof
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BgIII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Article Title: Treatment and diagnosis of colon cancer
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BglII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Article Title: Potential effects of endogenous RNA/DNA hybrids on CRISPR-Cas9-mediated homology-directed repair
    Article Snippet: .. gRNA expression plasmids were generated using scAAV-sgRNA-GFP (Addgene plasmid #177935). ..

    Article Title: Treatment and diagnosis of colon cancer
    Article Snippet: .. Adeno-Associated Viral Therapy miR-483-5p and miR-551a were cloned as a polycistron consisting of both miRNA precursor with flanking genomic sequences in tandem into the BglII and NotI site of scAAV.GFP (Plasmid 21893, Addgene). ..

    Expressing:

    Article Title: Potential effects of endogenous RNA/DNA hybrids on CRISPR-Cas9-mediated homology-directed repair
    Article Snippet: .. gRNA expression plasmids were generated using scAAV-sgRNA-GFP (Addgene plasmid #177935). ..

    Generated:

    Article Title: Potential effects of endogenous RNA/DNA hybrids on CRISPR-Cas9-mediated homology-directed repair
    Article Snippet: .. gRNA expression plasmids were generated using scAAV-sgRNA-GFP (Addgene plasmid #177935). ..



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    Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in <t>scAAV</t> (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).
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    Image Search Results


    Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

    Journal: iScience

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

    doi: 10.1016/j.isci.2024.110706

    Figure Lengend Snippet: Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

    Article Snippet: The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter.

    Techniques: Virus, Injection, Marker, Expressing, Transduction

    Journal: iScience

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

    doi: 10.1016/j.isci.2024.110706

    Figure Lengend Snippet:

    Article Snippet: The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter.

    Techniques: Recombinant, Software