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scaav8 cmv egfp  (PackGene Biotech lnc)


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    Structured Review

    PackGene Biotech lnc scaav8 cmv egfp
    Scaav8 Cmv Egfp, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/self+complementary+aav+vector/scAAV8+Vector+Production/pmc10978582-45-16-19
    Average 96 stars, based on 2 article reviews
    scaav8 cmv egfp - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses
    Article Snippet: .. Zygotes were incubated in drops of PZM3 containing the following rAAV vectors for 5–6h, scAAV1-CMV-EGFP, scAAV6-CMV-EGFP, scAAV8-CMV-EGFP and scAAV1-CMV-EGFP (Pack gene, China) at a viral load of 5 × 10 9 GC to analyze the effect of multiple rAAV serotypes for transfection of porcine embryos; scAAV6-CMV-EGFP at 5 × 10 7 GC, 5 × 10 9 GC, 5 × 10 10 GC, and 5 × 10 11 GC to screen the optimal virus transfection dose; rAAV6-CRISPR/Cas9 target gene vectors carrying an EGFP-tagged were used to test the efficiency of rAAV-CRISPR/Cas9-mediated in vitro embryonic gene editing (scAAV6-U6-sgGHR-CMV-EGFP and ssAAV6-U1a-spCas9, purchased from Pack gene, at 5 × 10 10 GC); Drops were inserted into 35 mm plates, which were submerged in mineral oil (Sigma, M8410) at 37 °C in a tissue culture incubator with 5% CO 2 and 5% O 2 . ..

    Transfection:

    Article Title: One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses
    Article Snippet: .. Zygotes were incubated in drops of PZM3 containing the following rAAV vectors for 5–6h, scAAV1-CMV-EGFP, scAAV6-CMV-EGFP, scAAV8-CMV-EGFP and scAAV1-CMV-EGFP (Pack gene, China) at a viral load of 5 × 10 9 GC to analyze the effect of multiple rAAV serotypes for transfection of porcine embryos; scAAV6-CMV-EGFP at 5 × 10 7 GC, 5 × 10 9 GC, 5 × 10 10 GC, and 5 × 10 11 GC to screen the optimal virus transfection dose; rAAV6-CRISPR/Cas9 target gene vectors carrying an EGFP-tagged were used to test the efficiency of rAAV-CRISPR/Cas9-mediated in vitro embryonic gene editing (scAAV6-U6-sgGHR-CMV-EGFP and ssAAV6-U1a-spCas9, purchased from Pack gene, at 5 × 10 10 GC); Drops were inserted into 35 mm plates, which were submerged in mineral oil (Sigma, M8410) at 37 °C in a tissue culture incubator with 5% CO 2 and 5% O 2 . ..

    Virus:

    Article Title: One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses
    Article Snippet: .. Zygotes were incubated in drops of PZM3 containing the following rAAV vectors for 5–6h, scAAV1-CMV-EGFP, scAAV6-CMV-EGFP, scAAV8-CMV-EGFP and scAAV1-CMV-EGFP (Pack gene, China) at a viral load of 5 × 10 9 GC to analyze the effect of multiple rAAV serotypes for transfection of porcine embryos; scAAV6-CMV-EGFP at 5 × 10 7 GC, 5 × 10 9 GC, 5 × 10 10 GC, and 5 × 10 11 GC to screen the optimal virus transfection dose; rAAV6-CRISPR/Cas9 target gene vectors carrying an EGFP-tagged were used to test the efficiency of rAAV-CRISPR/Cas9-mediated in vitro embryonic gene editing (scAAV6-U6-sgGHR-CMV-EGFP and ssAAV6-U1a-spCas9, purchased from Pack gene, at 5 × 10 10 GC); Drops were inserted into 35 mm plates, which were submerged in mineral oil (Sigma, M8410) at 37 °C in a tissue culture incubator with 5% CO 2 and 5% O 2 . ..

    In Vitro:

    Article Title: One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses
    Article Snippet: .. Zygotes were incubated in drops of PZM3 containing the following rAAV vectors for 5–6h, scAAV1-CMV-EGFP, scAAV6-CMV-EGFP, scAAV8-CMV-EGFP and scAAV1-CMV-EGFP (Pack gene, China) at a viral load of 5 × 10 9 GC to analyze the effect of multiple rAAV serotypes for transfection of porcine embryos; scAAV6-CMV-EGFP at 5 × 10 7 GC, 5 × 10 9 GC, 5 × 10 10 GC, and 5 × 10 11 GC to screen the optimal virus transfection dose; rAAV6-CRISPR/Cas9 target gene vectors carrying an EGFP-tagged were used to test the efficiency of rAAV-CRISPR/Cas9-mediated in vitro embryonic gene editing (scAAV6-U6-sgGHR-CMV-EGFP and ssAAV6-U1a-spCas9, purchased from Pack gene, at 5 × 10 10 GC); Drops were inserted into 35 mm plates, which were submerged in mineral oil (Sigma, M8410) at 37 °C in a tissue culture incubator with 5% CO 2 and 5% O 2 . ..

    Plasmid Preparation:

    Article Title: Electric pulse exposure reduces AAV8 dosage required to transduce HepG2 cells
    Article Snippet: Viable cell counts were determined by manual cell counts using Trypan Blue (Lonza, Walkersville, MD, USA) exclusion. .. The AAV8 vector used in these experiments was scAAV8.CMV.EGFP.WPREs (Packgene Biotech, Worcester, MA, USA) which contains enhanced green fluorescent protein (EGFP) as the reporter and provides a convenient way to measure transduction efficiency via fluorescence microscopy. ..

    Transduction:

    Article Title: Electric pulse exposure reduces AAV8 dosage required to transduce HepG2 cells
    Article Snippet: Viable cell counts were determined by manual cell counts using Trypan Blue (Lonza, Walkersville, MD, USA) exclusion. .. The AAV8 vector used in these experiments was scAAV8.CMV.EGFP.WPREs (Packgene Biotech, Worcester, MA, USA) which contains enhanced green fluorescent protein (EGFP) as the reporter and provides a convenient way to measure transduction efficiency via fluorescence microscopy. ..

    Fluorescence:

    Article Title: Electric pulse exposure reduces AAV8 dosage required to transduce HepG2 cells
    Article Snippet: Viable cell counts were determined by manual cell counts using Trypan Blue (Lonza, Walkersville, MD, USA) exclusion. .. The AAV8 vector used in these experiments was scAAV8.CMV.EGFP.WPREs (Packgene Biotech, Worcester, MA, USA) which contains enhanced green fluorescent protein (EGFP) as the reporter and provides a convenient way to measure transduction efficiency via fluorescence microscopy. ..

    Microscopy:

    Article Title: Electric pulse exposure reduces AAV8 dosage required to transduce HepG2 cells
    Article Snippet: Viable cell counts were determined by manual cell counts using Trypan Blue (Lonza, Walkersville, MD, USA) exclusion. .. The AAV8 vector used in these experiments was scAAV8.CMV.EGFP.WPREs (Packgene Biotech, Worcester, MA, USA) which contains enhanced green fluorescent protein (EGFP) as the reporter and provides a convenient way to measure transduction efficiency via fluorescence microscopy. ..



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    Image Search Results


    Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

    Journal: iScience

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

    doi: 10.1016/j.isci.2024.110706

    Figure Lengend Snippet: Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

    Article Snippet: The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter.

    Techniques: Virus, Injection, Marker, Expressing, Transduction

    Journal: iScience

    Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

    doi: 10.1016/j.isci.2024.110706

    Figure Lengend Snippet:

    Article Snippet: The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter.

    Techniques: Recombinant, Software