insulin transferrin selenium (Thermo Fisher)

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Thermo Fisher insulin transferrin selenium
Insulin Transferrin Selenium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selenium/Transferrin/pmc13099361-378-21-23
Average 99 stars, based on 1 article reviews

insulin transferrin selenium - by Bioz Stars, 2026-11

99/100 stars

Images

Membrane:

Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method
Article Snippet: Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, mouse tissue (Miltenyi Biotec, Cat#130-096-946) Myelin Removal Beads II, human, mouse, rat (Miltenyi Biotec, Cat#130-096-433) Dulbecco’s PBS (1X), without Ca and Mg, without Phenol Red (Capricorn Scientific, Cat#PBS-1A) Roche cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail (Roche, Cat#11836170001) Bovine Serum Albumin heat shock fraction, pH 7, ≥ 98 % (Sigma-Aldrich, Cat#A7906) BioTracker 633 Red Mitochondria Dye (Sigma-Aldrich, Cat#SCT137) SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific, Cat#34096) Pierce BCA Protein Assay Kit (Thermo Scientific, Cat#23227) .. anti-TOM20 (Translocase of outer mitochondrial membrane 20 homolog), rabbit recombinant monoclonal antibody (Proteintech, Cat#80501-1-RR), dilution 1:5000 anti-PMCA (Plasma membrane-type Ca 2+ -ATPase), mouse monoclonal antibody (Santa Cruz Biotechnology, Cat#SC-271917), dilution 1:1000 anti-TfR (Human transferrin receptor), mouse polyclonal antibody (Thermo Fisher, Cat13–6800), dilution 1:2000 anti-Thy1 (Thymus cell antigen 1, theta), rat polyclonal antibody (BD Biosciences, Cat#553000), dilution 1:1000 anti-SIGMAR1 (Sigma non-opioid intracellular receptor 1), rabbit polyclonal antibody (Proteintech, Cat#15168-1-AP), dilution 1:4000 anti-PDHA2 (Pyruvate dehydrogenase (lipoamide) alpha 2), rabbit polyclonal antibody (Proteintech, Cat#17134-1-AP), dilution 1:3000 anti-PEX3 (Peroxisomal biogenesis factor 3), rabbit polyclonal antibody (Proteintech, Cat#30,424-1-AP), dilution 1:750 anti-CALR (Calreticulin), rabbit polyclonal antibody (Proteintech, Cat#10,292-1-AP), dilution 1:1250 .. Peroxidase AffiniPure Goat Anti-Rabbit IgG ( H + L ) (Jackson Immunoresearch, Cat#111–035–003), dilution 1:50 000 Peroxidase AffiniPure Donkey Anti-Mouse IgG ( H + L ) (Jackson Immunoresearch, Cat#715–035–150), dilution 1:50 000 Peroxidase AffiniPure Donkey Anti-Rat IgG ( H + L ) (Jackson Immunoresearch, Cat#712–035–153), dilution 1:50 000 Alexa Fluor 488 AffiniPure Goat Anti-Rabbit IgG ( H + L ) (Jackson Immunoresearch, Cat#111–545–003), dilution 1:400

Recombinant:

Article Title: Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells
Article Snippet: .. As described previously, hTSC lines were established from isolated villous cytotrophoblasts of human single placentae. hTSC lines were maintained on human fibronectin (Sigma) pre-coated surface in advanced DMEM/F12 (Invitrogen) containing 10 mM HEPES (Gibco), 1×B-27 Supplement (Gibco), 1×Insulin-Transferrin-Selenium-Ethanolamine (ITS-X, Gibco), and 2 mM Glutamine (Gibco), 100 ng/mL recombinant human epidermal growth factor (rhEGF, R&D Systems), 3 μM CHIR99021 (Tocris), and 5 μM Rock Inhibitor (Y-27632, Santa Cruz) at 37°C with 5% CO 2 . ..

Article Title: Methods for preparing mesenchymal stem cells
Article Snippet: .. TABLE 1 Reagent information Name Manufacturer Item number Matrigel Corning 354277 IMDM Thermofisher 12440053 MEM non-essential Thermo 11140-050 amino acids solution (100X)) DMEM/F-12 with HEPES Thermo 11330-032 GlutaMAX-I(100X) Gibco 35050061 Insulin-transferrin-selenium-X Gibco 51500056 L-Ascorbic acid sigma A92902 BMP4 Peprotech 120-05ET Animal-free recombinant peprotech AF-100-18B human FGF-basic (154 a.a.) EGF sigma SRP3027 CHIR99021 StemCell Technologies 72054 SB431542 abcam ab120163 0.25% Trypsin-EDTA(1X) Thermo 25200072 KnockOut TM serum replacement ThermoFisher A3181502 A-83-01 Selleck S7692 Valproic acid Selleck S3944 Y-27632 2HCL Selleck S1049 Helios HPCPLCRL05 serum HELIOS HPCPLCRL05 replacement CellMax premium Sai Aomei Cell SA211.02 fetal bovine serum Technology (Beijing) Co. ..

Clinical Proteomics:

Modification:

Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung
Article Snippet: For 40 ml: 30 ml DMEM/F12, phenol red–free (Corning, 16‐405‐CV) 10 ml DMEM, high glucose (Corning, 10‐013‐CV) Store up to 1 month at 4°C .. For 40 ml: 35.2 ml Dulbecco's modified Eagle medium F12 (DMEM‐F12), phenol red–free (Corning, 16‐405‐CV) 4 ml of 0.2‐μm filtered fetal bovine serum (FBS) (10% final) (HyClone FBS, Thermo Fisher Scientific, SH3091003) 400 μl of 100× insulin‐transferrin‐selenium (ITS) (Gibco, 41400‐045) 400 μl of 100× antibiotic‐antimycotic (Gibco, 15240‐062) Store up to 1 month at 4°C .. For 500 ml: 3 ml acid citrate‐dextrose solution (ACD) (Sigma‐Aldrich, C3821) 497 ml of 1× DPBS (Corning, 21‐031‐CV) 2.5 g bovine serum albumin (BSA) (BD, 354331) Store up to 3 months at 4°C Before use, check for bacterial contamination or any other growth by swirling the bottle and inspecting from the bottom for debris.

Isolation:

Knock-Out:

Cell Culture:

Article Title: In silico discovery of nanobody binders to a G-protein coupled receptor using AlphaFold-Multimer.
Article Snippet: .. Rosa cells were cultured at 37 °C and 5% CO2 in IMDM supplemented with 1% Penicillin-Streptomycin (Pen-Strep), 1% sodium pyruvate (GIBCO), 1% MEM (minimal essential medium) (GIBCO), 2% MEM non-essential amino acids (GIBCO), 1% L-glutamine (GIBCO), 1% insulin transferrin-sodium selenite (GIBCO), 0.3% bovine serum albumin, with 80 ng/mL fresh mouse stem cell factor (SCF) AR TI CL E IN P RE SS ARTICLE IN PRESS (R&D Systems). .. Rosa cells were cultured at 37 °C and 5% CO2 in IMDM supplemented with 1% Penicillin-Streptomycin (Pen-Strep), 1% sodium pyruvate (GIBCO), 1% MEM (minimal essential medium) (GIBCO), 2% MEM non-essential amino acids (GIBCO), 1% L-glutamine (GIBCO), 1% insulin transferrin-sodium selenite (GIBCO), 0.3% bovine serum albumin, with 80 ng/mL fresh mouse stem cell factor (SCF) AR TI CL E IN P RE SS ARTICLE IN PRESS (R&D Systems).

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