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puromycin selection  (InvivoGen)


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    Structured Review

    InvivoGen puromycin selection
    Puromycin Selection, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 4345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/selection/Puromycin/pmc13655375-57-5-9
    Average 99 stars, based on 4345 article reviews
    puromycin selection - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.
    Article Snippet: .. One day post transfection, the drug selection was initiated by replacing the spent media with fresh media containing the appropriate selective antibiotic for the construct: Puromycin (50 μg/mL, InvivoGen, Cat. #ant-pr-5b) or Blasticidin (70 μg/mL, InvivoGen, Cat. # ant-bl-5b). ..

    Article Title: TBK1 eliminates aggregation-prone monomeric TDP-43 through an IFNβ-immunoproteasome pathway in ALS models.
    Article Snippet: The first transfection was performed using linearized pTet-On Advanced vector (#630930, Takara Bio), followed by positive selection with 1 mg/mL G-418 (Nacalai Tesque) for 2 weeks. .. The second transfection was then performed using linearized pTRE-TighthTDP-43 (WT or 6M)-3×FLAG-SV40-PuroR, followed by positive selection with 2 μg/mL puromycin (InvivoGen, San Diego, CA, USA) for an additional two weeks. .. The first transfection was performed using linearized pTet-On Advanced vector (#630930, Takara Bio), followed by positive selection with 1 mg/mL G-418 (Nacalai Tesque) for 2 weeks.

    Article Title: Tumor-intrinsic IFNγ signaling and niche adaptation drive early colonization in ovarian cancer metastasis.
    Article Snippet: .. 48 hours post transfection, cells were selected with either G418 (MetTag-LARRY-GFP lines; Sigma-Aldrich, 4727878001) or puromycin (CROP-seq-V2.mCherry lines; Invivogen, ant-pr-1) for a minimum of 7 days. .. For clonal and time stamp (BC.ID) tracking, all barcoded pools of cells were infected simultaneously using the same cell passage, same lentivirus titer as measured by a Lenti-X GoStix Plus kit (Takara Bio, 631280) and were thawed in a staggered manner for Nature Communications | (2026) 17:10497 15 injection.

    Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.
    Article Snippet: .. 24 h post transfection with the CRISPR constructs, the cells were selected in culture medium containing 2.5 μg/ml puromycin (Invivogen) for 4 days, and then cultivated for 24 h in the absence of puromycin. ..

    Selection:

    Article Title: A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.
    Article Snippet: .. One day post transfection, the drug selection was initiated by replacing the spent media with fresh media containing the appropriate selective antibiotic for the construct: Puromycin (50 μg/mL, InvivoGen, Cat. #ant-pr-5b) or Blasticidin (70 μg/mL, InvivoGen, Cat. # ant-bl-5b). ..

    Article Title: PYGO2 ablation promotes lymphoid organization and STING-dependent antitumor immunity in prostate cancer.
    Article Snippet: Prostate cancer is an immunologically cold tumor with a limited response to immune checkpoint blockade.. Although tertiary lymphoid structures (TLSs) are associated with favorable immunotherapeutic outcomes in various malignancies, the tumor-intrinsic mechanisms governing their formation in prostate cancer remain poorly defined.. We investigated whether the frequently amplified chromatin effector PYGO2 regulates lymphoid organization and antitumor immunity.

    Article Title: TBK1 eliminates aggregation-prone monomeric TDP-43 through an IFNβ-immunoproteasome pathway in ALS models.
    Article Snippet: The first transfection was performed using linearized pTet-On Advanced vector (#630930, Takara Bio), followed by positive selection with 1 mg/mL G-418 (Nacalai Tesque) for 2 weeks. .. The second transfection was then performed using linearized pTRE-TighthTDP-43 (WT or 6M)-3×FLAG-SV40-PuroR, followed by positive selection with 2 μg/mL puromycin (InvivoGen, San Diego, CA, USA) for an additional two weeks. .. The first transfection was performed using linearized pTet-On Advanced vector (#630930, Takara Bio), followed by positive selection with 1 mg/mL G-418 (Nacalai Tesque) for 2 weeks.

    Article Title: Integrated Transcriptomic Analysis Identifies ENO3 as a Candidate Lactate Metabolism‐Associated Biomarker in Colorectal Cancer Metastasis
    Article Snippet: Culture supernatants were collected 48 h later, passed through 0.45‐μm filters (Millipore #SLHV033RB), and applied to MC38 cells. .. Transduced populations were enriched by puromycin selection (5 μg/mL, InvivoGen #ant‐pr‐1) over 7 days. .. Monoclonal lines were isolated by fluorescence‐activated cell sorting (BD FACSAria III), and loss of ENO3 protein was verified by immunoblotting.

    Article Title: Developmentally Guided Differentiation of Ventral Midbrain Dopaminergic Cells Validated in Proteopathy-Based Parkinson's Disease Models.
    Article Snippet: .. Four ays post-electroporation, puromycin selection was initiated by dding 0.5 μg/mL puromycin (InvivoGen, ant-pr-1) to the hPSC edium. ..

    Construct:

    Article Title: A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.
    Article Snippet: .. One day post transfection, the drug selection was initiated by replacing the spent media with fresh media containing the appropriate selective antibiotic for the construct: Puromycin (50 μg/mL, InvivoGen, Cat. #ant-pr-5b) or Blasticidin (70 μg/mL, InvivoGen, Cat. # ant-bl-5b). ..

    Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.
    Article Snippet: .. 24 h post transfection with the CRISPR constructs, the cells were selected in culture medium containing 2.5 μg/ml puromycin (Invivogen) for 4 days, and then cultivated for 24 h in the absence of puromycin. ..

    Single Cell:

    Article Title: PYGO2 ablation promotes lymphoid organization and STING-dependent antitumor immunity in prostate cancer.
    Article Snippet: Prostate cancer is an immunologically cold tumor with a limited response to immune checkpoint blockade.. Although tertiary lymphoid structures (TLSs) are associated with favorable immunotherapeutic outcomes in various malignancies, the tumor-intrinsic mechanisms governing their formation in prostate cancer remain poorly defined.. We investigated whether the frequently amplified chromatin effector PYGO2 regulates lymphoid organization and antitumor immunity.

    Clone Assay:

    Article Title: PYGO2 ablation promotes lymphoid organization and STING-dependent antitumor immunity in prostate cancer.
    Article Snippet: Prostate cancer is an immunologically cold tumor with a limited response to immune checkpoint blockade.. Although tertiary lymphoid structures (TLSs) are associated with favorable immunotherapeutic outcomes in various malignancies, the tumor-intrinsic mechanisms governing their formation in prostate cancer remain poorly defined.. We investigated whether the frequently amplified chromatin effector PYGO2 regulates lymphoid organization and antitumor immunity.

    Isolation:

    Article Title: PYGO2 ablation promotes lymphoid organization and STING-dependent antitumor immunity in prostate cancer.
    Article Snippet: Prostate cancer is an immunologically cold tumor with a limited response to immune checkpoint blockade.. Although tertiary lymphoid structures (TLSs) are associated with favorable immunotherapeutic outcomes in various malignancies, the tumor-intrinsic mechanisms governing their formation in prostate cancer remain poorly defined.. We investigated whether the frequently amplified chromatin effector PYGO2 regulates lymphoid organization and antitumor immunity.

    CRISPR:

    Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.
    Article Snippet: .. 24 h post transfection with the CRISPR constructs, the cells were selected in culture medium containing 2.5 μg/ml puromycin (Invivogen) for 4 days, and then cultivated for 24 h in the absence of puromycin. ..



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    Image Search Results


    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: NucleoMag NGS Clean-up and Size Select Beads , Macherey-Nagel , 744970.50.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software