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BioPioneer Inc sds-page loading buffer
Sds Page Loading Buffer, supplied by BioPioneer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds-page+loading+buffer/sds+page+loading+buffer/pmc05889092-716-66-69
Average 90 stars, based on 1 article reviews
sds-page loading buffer - by Bioz Stars, 2026-11
90/100 stars

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Immunoprecipitation:

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: Immunoprecipitation assay and Aβ ELISA In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Enzyme-linked Immunosorbent Assay:

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: Immunoprecipitation assay and Aβ ELISA In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Binding Assay:

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: Immunoprecipitation assay and Aβ ELISA In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Incubation:

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: Immunoprecipitation assay and Aβ ELISA In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

SDS Page:

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: Immunoprecipitation assay and Aβ ELISA In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Western Blot:

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.

Article Title: TREM2 is a receptor for β-amyloid which mediates microglial function
Article Snippet: Immunoprecipitation assay and Aβ ELISA In cell-free binding assays, 0.5 μg human IgG1 Fc, TREM2-Fc or TREM1-Fc (R&D Systems) was incubated with 0.25 μg unlabeled or biotin-labeled Aβ monomer or oligomers in TBS buffer (25 mM Tris-HCl pH 7.6, 150 mM sodium chloride) containing 0.05% Tween-20 (TBST) and Dynabeads protein G (Thermo Fisher Scientific) at 4°C for 2 h. Beads were then washed with TBST fo ur times and boiled in SDS-PAGE loading buffer (BioPioneer) containing 62.6 mM Tris-HCl (pH 6.8), 2% SDS, 0.01% bromophenol blue, 10% glycerol and 0.5M dithiothreitol and subjected to immunoblot analysis.



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