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anti ubiquitin antibodies  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti ubiquitin antibodies
    a Western blot of PIF4a-HA fusion protein in PIF4a-HA overexpressing plants in response to red light at 21 °C and 15 °C using an anti-HA antibody to detect PIF4a-HA and an anti-RPN6 antibody as loading controls. 4-week-old plants were kept in the dark at 21 °C for 24 h and then transferred into two cabinets with 10 μmol m −2 s −1 red light at either 21 °C or 15 °C. Leave samples were taken at 0, 10, 30, and 60 min of red light treatment at two temperatures. The bottom panel shows the quantification of PIF4a-HA fusion protein by western blot. Data are represented as mean ± SEM, n = 3. The asterisks represent significance levels (* p < 0.05) based on Fisher’s LSD test (two-sided) without multiple testing correction following ANOVA. b TUBEs (tandem <t>ubiquitin-binding</t> entities) assays of ubiquitinated proteins in the samples from ( a ) after 30 and 60 min of red light treatment. Total ubiquitinated proteins from samples were immunoprecipitated with agarose-TUBE2, then analyzed by western blotting with anti-HA antibodies for detection of PIF4-HA and anti-ubiquitin antibodies as loading controls. Control agarose that was not TUBEs-conjugated was used as a negative control. Anti-RPN6 antibodies were used as loading controls for input samples. c Co-immunoprecipitation assays of PHYB2 and PIF4a interaction in 35S::PIF4a-HA-1/35S::PHYB2-GFP trees at 21 °C and 15 °C. Co-immunoprecipitated PIF4a-HA was detected by western blotting using anti-HA antibodies, after immunoprecipitation of PHYB2-GFP by GFP-trap beads. Precipitate probed with anti-GFP antibodies and input samples are shown as controls. d TUBEs assays of ubiquitinated proteins in 35S::PIF4a-HA-1 and 35S::PIF4a-HA-1/35S::PHYB2-GFP trees at two temperatures. Leaf samples were taken at ZT8 of 6-week-old LD grown trees. One short-time exposed image and one long-time exposed image are shown from the same blot. See also Supplementary Fig. . Source data are provided as a file.
    Anti Ubiquitin Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+loading+buffer+(2x+laemlli+sample+buffer/Ubiquitin+Antibody/pmc12398530-340-34-37
    Average 96 stars, based on 5397 article reviews
    anti ubiquitin antibodies - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Phytochrome B and phytochrome-interacting-factor4 modulate tree seasonal growth in cold environments"

    Article Title: Phytochrome B and phytochrome-interacting-factor4 modulate tree seasonal growth in cold environments

    Journal: Nature Communications

    doi: 10.1038/s41467-025-63391-5

    a Western blot of PIF4a-HA fusion protein in PIF4a-HA overexpressing plants in response to red light at 21 °C and 15 °C using an anti-HA antibody to detect PIF4a-HA and an anti-RPN6 antibody as loading controls. 4-week-old plants were kept in the dark at 21 °C for 24 h and then transferred into two cabinets with 10 μmol m −2 s −1 red light at either 21 °C or 15 °C. Leave samples were taken at 0, 10, 30, and 60 min of red light treatment at two temperatures. The bottom panel shows the quantification of PIF4a-HA fusion protein by western blot. Data are represented as mean ± SEM, n = 3. The asterisks represent significance levels (* p < 0.05) based on Fisher’s LSD test (two-sided) without multiple testing correction following ANOVA. b TUBEs (tandem ubiquitin-binding entities) assays of ubiquitinated proteins in the samples from ( a ) after 30 and 60 min of red light treatment. Total ubiquitinated proteins from samples were immunoprecipitated with agarose-TUBE2, then analyzed by western blotting with anti-HA antibodies for detection of PIF4-HA and anti-ubiquitin antibodies as loading controls. Control agarose that was not TUBEs-conjugated was used as a negative control. Anti-RPN6 antibodies were used as loading controls for input samples. c Co-immunoprecipitation assays of PHYB2 and PIF4a interaction in 35S::PIF4a-HA-1/35S::PHYB2-GFP trees at 21 °C and 15 °C. Co-immunoprecipitated PIF4a-HA was detected by western blotting using anti-HA antibodies, after immunoprecipitation of PHYB2-GFP by GFP-trap beads. Precipitate probed with anti-GFP antibodies and input samples are shown as controls. d TUBEs assays of ubiquitinated proteins in 35S::PIF4a-HA-1 and 35S::PIF4a-HA-1/35S::PHYB2-GFP trees at two temperatures. Leaf samples were taken at ZT8 of 6-week-old LD grown trees. One short-time exposed image and one long-time exposed image are shown from the same blot. See also Supplementary Fig. . Source data are provided as a file.
    Figure Legend Snippet: a Western blot of PIF4a-HA fusion protein in PIF4a-HA overexpressing plants in response to red light at 21 °C and 15 °C using an anti-HA antibody to detect PIF4a-HA and an anti-RPN6 antibody as loading controls. 4-week-old plants were kept in the dark at 21 °C for 24 h and then transferred into two cabinets with 10 μmol m −2 s −1 red light at either 21 °C or 15 °C. Leave samples were taken at 0, 10, 30, and 60 min of red light treatment at two temperatures. The bottom panel shows the quantification of PIF4a-HA fusion protein by western blot. Data are represented as mean ± SEM, n = 3. The asterisks represent significance levels (* p < 0.05) based on Fisher’s LSD test (two-sided) without multiple testing correction following ANOVA. b TUBEs (tandem ubiquitin-binding entities) assays of ubiquitinated proteins in the samples from ( a ) after 30 and 60 min of red light treatment. Total ubiquitinated proteins from samples were immunoprecipitated with agarose-TUBE2, then analyzed by western blotting with anti-HA antibodies for detection of PIF4-HA and anti-ubiquitin antibodies as loading controls. Control agarose that was not TUBEs-conjugated was used as a negative control. Anti-RPN6 antibodies were used as loading controls for input samples. c Co-immunoprecipitation assays of PHYB2 and PIF4a interaction in 35S::PIF4a-HA-1/35S::PHYB2-GFP trees at 21 °C and 15 °C. Co-immunoprecipitated PIF4a-HA was detected by western blotting using anti-HA antibodies, after immunoprecipitation of PHYB2-GFP by GFP-trap beads. Precipitate probed with anti-GFP antibodies and input samples are shown as controls. d TUBEs assays of ubiquitinated proteins in 35S::PIF4a-HA-1 and 35S::PIF4a-HA-1/35S::PHYB2-GFP trees at two temperatures. Leaf samples were taken at ZT8 of 6-week-old LD grown trees. One short-time exposed image and one long-time exposed image are shown from the same blot. See also Supplementary Fig. . Source data are provided as a file.

    Techniques Used: Western Blot, Ubiquitin Proteomics, Binding Assay, Immunoprecipitation, Control, Negative Control

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    Cell-Signaling:

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    Article Snippet: Glioblastoma (GBM) is a highly aggressive primary brain tumor with grim overall median survival, and its therapeutic efficacy is severely limited due to drug resistance.. Therefore, developing effective and safe chemotherapy regimens is urgently required.. Juglone, a natural bioactive constituent derived from traditional Chinese herbs, has emerged as a promising therapeutic agent against various human malignancies.

    Article Title: CSN-associated c-Cbl control alternative NF-κB/RelB activity in Helicobacter pylori infection.
    Article Snippet: Membranes were washed again thrice with TBS-T prior to detection with a chemiluminescent HRP substrate (Merck Millipore, WBKLS0500) using the ChemoCam Imager (Intas). .. The following primary antibodies were used: c-Cbl (05–440, upstate), GAPDH (MAB374, Millipore), CSN2 (ab10426) and CSN3 (ab79698) were ordered from Abcam, CSN5 (#6895), IκBα (#4812), p-IκBα (#2859), p100/p52 (#4882), p-p100 (#4810), RelB (#10544), β-TRCP (#4394) and K48-ubiquitin specific (#8081) were ordered from Cell Signaling Technology, while C23 (sc-8031), Cullin-1 (sc-17775), fibrillarin (Sc166001), RelA (sc-8008), and Pan-ubiquitin (sc-8017) were obtained from Santa Cruz Biotechnology. .. Total RNA extraction was performed with the NucleoSpin RNA Plus Kit (Marchery-Nagel, 740984.250) and RNA was reverse-transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1622).

    Article Title: A chimeric murine coronavirus with altered deISGylase and proteolytic activities exhibits attenuated viral pathogenesis.
    Article Snippet: Blots visualizing ISG15 were devel oped using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific Cat #34095). .. The following antibodies were used in this study: mouse anti-HA.11 Month XXXX Volume 0 Issue 0 10.1128/jvi.00857-2616 h ttp s: //j ou rn al s. as m .o rg /jo ur na l/j vi o n 02 O ct ob er 2 02 6 by 2 80 4: 1b 3: a9 02 :c 0e a: e1 5a :9 3a 7: a8 42 :9 b2 7. (1:2,000, BioLegend 901513), mouse anti-FLAG M2 (1:2,000, Sigma-Aldrich F1804), mouse anti-V5 (1:5,000, Invitrogen R960-25), mouse anti-ISG15 (1:1,000, Santa Cruz Biotechnol ogy), mouse anti-β-actin (1:5,000, GenScript A00702-40), anti-MDA5 antibody (1:1,000, Cell Signaling Technology D74E4), anti-ubiquitin antibody (1:500, Santa Cruz P4D1), anti-K48-linkage-specific polyubiquitin antibody (1:1,000, Cell Signaling Technology D9D5), and mouse anti-MHV nucleocapsid (1:5,000, J.3.3, a gift from John Fleming at the University of Wisconsin-Madison) .. The following secondary antibodies were used: HRP-conjugated goat anti-mouse (1:5,000, SouthernBiotech 1010-05) and HRP-conjuga ted donkey anti-rabbit (1:5,000, Jackson ImmunoResearch 711-035-152).

    Article Title: RAD54L counteracts topoisomerase I cleavage complexes by stabilizing E3 ligase TRIM21 to maintain genome stability
    Article Snippet: The blots were detected using an ECL reagent (Millipore) and captured with the ChemiDoc MP Imaging System (Bio-Rad). .. The primary antibodies used for DUST assay were mouse anti-TOP1 (1:200 dilution, Santa Cruz sc-271285), mouse anti-Ubiquitin (1:100 dilution, Santa Cruz sc-8017), mouse anti-MYC (1:1000 dilution, Cell Signaling Technology 2276), mouse anti-dsDNA (1:500 dilution, Santa Cruz sc-58749). .. Total RNA was extracted from HEK293 cells using the RNA-Quick Purification Kit (ES Science) and reversetranscribed into cDNA using the PrimeScript RT Reagent Kit (Takara).

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

    Ubiquitin Proteomics:

    Article Title: Juglone induces GSDME-mediated pyroptosis by targeting HSP90α ATPase domain to promote STAT3 degradation in glioblastoma multiforme.
    Article Snippet: Glioblastoma (GBM) is a highly aggressive primary brain tumor with grim overall median survival, and its therapeutic efficacy is severely limited due to drug resistance.. Therefore, developing effective and safe chemotherapy regimens is urgently required.. Juglone, a natural bioactive constituent derived from traditional Chinese herbs, has emerged as a promising therapeutic agent against various human malignancies.

    Western Blot:

    Article Title: Distinct ways by which Srs2 helicase and Rrp1 translocase shape Rad51-DNA interactions in fission yeast.
    Article Snippet: Samples were boiled before being subjected to SDS–polyacrylamide gel electrophoresis (PAGE) on Mini-PROTEAN TGX Precast Gel 4%–15% (Bio-Rad, 4561086). .. Western blots were performed with following antibodies at relevant dilutions: 1:1000 anti-GFP (Roche, 11814460001), 1:3000 anti-HA (Sigma–Aldrich, H6908), 1:5000 anti-Rad51 (Abcam, ab63799), 1:2000 anti-FLAG (Sigma–Aldrich, F7425), 1:500 anti-ubiquitin (P4D1, Santa Cruz Biotechnology, sc-8017), and anti-PCNA (PC10, Santa Cruz Biotechnology, sc-56). ..

    Incubation:

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

    Purification:

    Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.



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